Optical control of an ion channel gate.

Abstract:

:The powerful optogenetic pharmacology method allows the optical control of neuronal activity by photoswitchable ligands tethered to channels and receptors. However, this approach is technically demanding, as it requires the design of pharmacologically active ligands. The development of versatile technologies therefore represents a challenging issue. Here, we present optogating, a method in which the gating machinery of an ATP-activated P2X channel was reprogrammed to respond to light. We found that channels covalently modified by azobenzene-containing reagents at the transmembrane segments could be reversibly turned on and off by light, without the need of ATP, thus revealing an agonist-independent, light-induced gating mechanism. We demonstrate photocontrol of neuronal activity by a light-gated, ATP-insensitive P2X receptor, providing an original tool devoid of endogenous sensitivity to delineate P2X signaling in normal and pathological states. These findings open new avenues to specifically activate other ion channels independently of their natural stimulus.

authors

Lemoine D,Habermacher C,Martz A,Méry PF,Bouquier N,Diverchy F,Taly A,Rassendren F,Specht A,Grutter T

doi

10.1073/pnas.1318715110

subject

Has Abstract

pub_date

2013-12-17 00:00:00

pages

20813-8

issue

51

eissn

0027-8424

issn

1091-6490

pii

1318715110

journal_volume

110

pub_type

杂志文章
  • Synergistic anti-human immunodeficiency virus type 1 effect of hydroxamate compounds with 2',3'-dideoxyinosine in infected resting human lymphocytes.

    abstract::The cellular models generally used in the in vitro evaluation of anti-human immunodeficiency virus compounds are dividing cells. A model constituted by resting lymphocytes may more accurately reflect a drug's future efficacy in humans, since viral DNA synthesis is known to take place in quiescent cells, creating a res...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.23.11017

    authors: Malley SD,Grange JM,Hamedi-Sangsari F,Vila JR

    更新日期:1994-11-08 00:00:00

  • Self-enforcing strategies to deter free-riding in the climate change mitigation game and other repeated public good games.

    abstract::As the Copenhagen Accord indicates, most of the international community agrees that global mean temperature should not be allowed to rise more than two degrees Celsius above preindustrial levels to avoid unacceptable damages from climate change. The scientific evidence distilled in the Fourth Assessment Report of the ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1106265108

    authors: Heitzig J,Lessmann K,Zou Y

    更新日期:2011-09-20 00:00:00

  • Inhibition of the Mg(II).ATP-dependent phosphoprotein phosphatase by the regulatory subunit of cAMP-dependent protein kinase.

    abstract::We report potent inhibition of the Mg(II).ATP-dependent protein phosphatase, Fc.M, by the regulatory subunit dimer of type II cAMP-dependent protein kinase, RII2. The protein kinase catalytic subunit has no effect on phosphatase activity and is unable to substitute for kinase FA in the kinase FA- and Mg(II).ATP-mediat...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.22.7565

    authors: Jurgensen SR,Chock PB,Taylor S,Vandenheede JR,Merlevede W

    更新日期:1985-11-01 00:00:00

  • Supplemental ascorbate in the supportive treatment of cancer: Prolongation of survival times in terminal human cancer.

    abstract::Ascorbic acid metabolism is associated with a number of mechanisms known to be involved in host resistance to malignant disease. Cancer patients are significantly depleted of ascorbic acid, and in our opinion this demonstrable biochemical characteristic indicates a substantially increased requirement and utilization o...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.73.10.3685

    authors: Cameron E,Pauling L

    更新日期:1976-10-01 00:00:00

  • Stable expression of rat cytochrome P-450IIB1 cDNA in Chinese hamster cells (V79) and metabolic activation of aflatoxin B1.

    abstract::V79 Chinese hamster fibroblasts are widely used for mutagenicity testing but have the serious limitation that they do not express cytochromes P-450, which are needed for the activation of many promutagens to mutagenic metabolites. A full-length cDNA clone encoding the monooxygenase cytochrome P-450IIB1 under control o...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.16.5769

    authors: Doehmer J,Dogra S,Friedberg T,Monier S,Adesnik M,Glatt H,Oesch F

    更新日期:1988-08-01 00:00:00

  • Ultrasensitivity of the Bacillus subtilis sporulation decision.

    abstract::Starving Bacillus subtilis cells execute a gene expression program resulting in the formation of stress-resistant spores. Sporulation master regulator, Spo0A, is activated by a phosphorelay and controls the expression of a multitude of genes, including the forespore-specific sigma factor σ(F) and the mother cell-speci...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1213974109

    authors: Narula J,Devi SN,Fujita M,Igoshin OA

    更新日期:2012-12-11 00:00:00

  • An 11-kDa form of human immunodeficiency virus protease expressed in Escherichia coli is sufficient for enzymatic activity.

    abstract::In order to define the protease domain of human immunodeficiency virus 1, various regions of the pol open reading frame were cloned and expressed in Escherichia coli. Antiserum directed against the conserved retroviral protease active site was used to identify pol precursor and processed species containing the presume...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.8.2449

    authors: Graves MC,Lim JJ,Heimer EP,Kramer RA

    更新日期:1988-04-01 00:00:00

  • Involvement of {beta}-catenin and unusual behavior of CBP and p300 in glucocorticosteroid signaling in Schwann cells.

    abstract::In the nervous system, glucocorticosteroid hormones play a major role during development and adult life. Myelin-forming cells are among the targets of glucocorticosteroids, which have been shown to promote myelination both in the central and peripheral nervous system. Glucocorticosteroid-stimulated gene transcription ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0506930102

    authors: Fonte C,Grenier J,Trousson A,Chauchereau A,Lahuna O,Baulieu EE,Schumacher M,Massaad C

    更新日期:2005-10-04 00:00:00

  • G1 cell cycle arrest and apoptosis induction by nuclear Smad4/Dpc4: phenotypes reversed by a tumorigenic mutation.

    abstract::The tumor suppressor Smad4/Dpc4 is a transcription activator that binds specific DNA sequences and whose nuclear localization is induced after exposure to type beta transforming growth factor-like cytokines. We explored an inducible system in which Smad4 protein is activated by translocation to the nucleus when cell l...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.4.1427

    authors: Dai JL,Bansal RK,Kern SE

    更新日期:1999-02-16 00:00:00

  • Global discovery of primate-specific genes in the human genome.

    abstract::The genomic basis of primate phenotypic uniqueness remains obscure, despite increasing genome and transcriptome sequence data availability. Although factors such as segmental duplications and positive selection have received much attention as potential drivers of primate phenotypes, single-copy primate-specific genes ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0904569106

    authors: Tay SK,Blythe J,Lipovich L

    更新日期:2009-07-21 00:00:00

  • Lack of p21 expression links cell cycle control and appendage regeneration in mice.

    abstract::Animals capable of regenerating multiple tissue types, organs, and appendages after injury are common yet sporadic and include some sponge, hydra, planarian, and salamander (i.e., newt and axolotl) species, but notably such regenerative capacity is rare in mammals. The adult MRL mouse strain is a rare exception to the...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1000830107

    authors: Bedelbaeva K,Snyder A,Gourevitch D,Clark L,Zhang XM,Leferovich J,Cheverud JM,Lieberman P,Heber-Katz E

    更新日期:2010-03-30 00:00:00

  • Gut-mediated hypercalcemia in rabbits bearing VX2 carcinoma: new mechanism for tumor-induced hypercalcemia.

    abstract::The VX2 carcinoma-bearing rabbit is an animal model for tumor-induced hypercalcemia, thought to be due to increased bone destruction effected by prostaglandin E2. The present experiments suggest that the pathophysiology of the hypercalcemia differs from that previously proposed. Tumor was transplanted intramuscularly ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.79.2.640

    authors: Doppelt SH,Slovik DM,Neer RM,Nolan J,Zusman RM,Potts JT Jr

    更新日期:1982-01-01 00:00:00

  • Does biased gene conversion influence polymorphism in the circumsporozoite protein-encoding gene of Plasmodium vivax?

    abstract::Variation between North Korean and Latin American isolates in the circumsporozoite (CS) protein encoding gene of the human malaria parasite Plasmodium vivax was studied. Polymorphic positions are confined to the central tandemly repeated sequences. Nucleotide substitutions in the tandem repeats produce variants; these...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.21.8102

    authors: Arnot DE,Barnwell JW,Stewart MJ

    更新日期:1988-11-01 00:00:00

  • Sigma subunit of Escherichia coli RNA polymerase affects the function of lambda N gene.

    abstract::A new class of Escherichia coli mutants, referred to as grn, has been isolated by localized mutagenesis. These mutations affect the sigma subunit of DNA-dependent RNA polymerase (ribonucleoside 5'-triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) by abolishing the expression of the lambda N gene, and they are close...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.9.4593

    authors: Nakamura Y,Kurihara T,Saito H,Uchida H

    更新日期:1979-09-01 00:00:00

  • Epstein-Barr virus co-opts TFIIH component XPB to specifically activate essential viral lytic promoters.

    abstract::Epstein-Barr virus (EBV) is associated with epithelial and lymphoid malignancies, establishes latent infection in memory B cells, and intermittently produces infectious virions through lytic replication. Released virions play a key role in latent reservoir maintenance and transmission. Lytic EBV transcription differs ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2000625117

    authors: Verma D,Church TM,Swaminathan S

    更新日期:2020-06-09 00:00:00

  • Minimal mechanistic model of siRNA-dependent target RNA slicing by recombinant human Argonaute 2 protein.

    abstract::Argonaute (Ago) proteins are the key component of the RNA-induced silencing complex and mediate RNA interference (RNAi) in association with small RNAs. Although overall the mechanism of RNAi is well understood, many molecular details of this complex process are not. Here we report about in-depth steady-state and, in p...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1217838110

    authors: Deerberg A,Willkomm S,Restle T

    更新日期:2013-10-29 00:00:00

  • Atomic model of the human cardiac muscle myosin filament.

    abstract::Of all the myosin filaments in muscle, the most important in terms of human health, and so far the least studied, are those in the human heart. Here we report a 3D single-particle analysis of electron micrograph images of negatively stained myosin filaments isolated from human cardiac muscle in the normal (undiseased)...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1212708110

    authors: Al-Khayat HA,Kensler RW,Squire JM,Marston SB,Morris EP

    更新日期:2013-01-02 00:00:00

  • Genome-wide identification of tissue-specific enhancers in the Ciona tadpole.

    abstract::Less than 100 cis-regulatory DNAs have been characterized in the context of transgenic metazoan embryos. Here we investigate the feasibility of conducting a genome-wide search for tissue-specific enhancers in the ascidian Ciona intestinalis. A total of 138 random genomic DNA fragments with an average size of 1.7 kb we...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.052024999

    authors: Harafuji N,Keys DN,Levine M

    更新日期:2002-05-14 00:00:00

  • The Drosophila 110-kDa transcription factor TFIID subunit directly interacts with the N-terminal region of the 230-kDa subunit.

    abstract::Transcription initiation factor TFIID is a multimeric protein complex that plays a central role in transcriptional regulation by facilitating promoter responses to various activators. cDNAs encoding the 110-kDa subunit of Drosophila TFIID (p110) were isolated with a degenerate oligodeoxynucleotide probe based on an am...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.13.5896

    authors: Kokubo T,Gong DW,Roeder RG,Horikoshi M,Nakatani Y

    更新日期:1993-07-01 00:00:00

  • P-hacking in clinical trials and how incentives shape the distribution of results across phases.

    abstract::Clinical research should conform to high standards of ethical and scientific integrity, given that human lives are at stake. However, economic incentives can generate conflicts of interest for investigators, who may be inclined to withhold unfavorable results or even tamper with data in order to achieve desired outcom...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1919906117

    authors: Adda J,Decker C,Ottaviani M

    更新日期:2020-06-16 00:00:00

  • The influence of sulfur and iron on dissolved arsenic concentrations in the shallow subsurface under changing redox conditions.

    abstract::The chemical speciation of arsenic in sediments and porewaters of aquifers is the critical factor that determines whether dissolved arsenic accumulates to potentially toxic levels. Sequestration of arsenic in solid phases, which may occur by adsorption or precipitation processes, controls dissolved concentrations. We ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0402775101

    authors: O'Day PA,Vlassopoulos D,Root R,Rivera N

    更新日期:2004-09-21 00:00:00

  • beta-Glucuronidase from Escherichia coli as a gene-fusion marker.

    abstract::We have developed a gene-fusion system based on the Escherichia coli beta-glucuronidase gene (uidA). The uidA gene has been cloned from E. coli K-12 and its entire nucleotide sequence has been determined. beta-Glucuronidase has been purified to homogeneity and characterized. The enzyme has a subunit molecular weight o...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.22.8447

    authors: Jefferson RA,Burgess SM,Hirsh D

    更新日期:1986-11-01 00:00:00

  • Genetic mapping of the inversion loop in bacteriophage Mu DNA.

    abstract::An inversion loop seen in heteroduplex mapping of the DNA of mature Mu phage induced from a lysogen is observed also in defective lambda phage carrying one end of Mu. 14% of the DNA of Mu, including this region, designated the G loop, is shown to be to the right of all known genes in the prophage map. The inhomogeneou...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.70.7.2153

    authors: Daniell E,Boram W,Abelson J

    更新日期:1973-07-01 00:00:00

  • Physical and functional interactions between Drosophila TRAF2 and Pelle kinase contribute to Dorsal activation.

    abstract::Signaling through the Toll receptor is required for dorsal/ventral polarity in Drosophila embryos, and also plays an evolutionarily conserved role in the immune response. Upon ligand binding, Toll appears to multimerize and activate the associated kinase, Pelle. However, the immediate downstream targets of Pelle have ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.141235698

    authors: Shen B,Liu H,Skolnik EY,Manley JL

    更新日期:2001-07-17 00:00:00

  • Pupil response as a predictor of blindsight in hemianopia.

    abstract::Significantly above-chance detection of stimuli presented within the field defect of patients with postgeniculate lesions is termed "blindsight." It has been proposed that those with blindsight are more likely to benefit from visual rehabilitation by repeated stimulation, leading to increased visual sensitivity within...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1318395110

    authors: Sahraie A,Trevethan CT,MacLeod MJ,Urquhart J,Weiskrantz L

    更新日期:2013-11-05 00:00:00

  • Inheritance of Trans Chromosomal Methylation patterns from Arabidopsis F1 hybrids.

    abstract::Hybridization in plants leads to transinteractions between the parental genomes and epigenomes that can result in changes to both 24 nt siRNA and cytosine methylation ((m)C) levels in the hybrid. In Arabidopsis the principle processes altering the hybrid methylome are Trans Chromosomal Methylation (TCM) and Trans Chro...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1323656111

    authors: Greaves IK,Groszmann M,Wang A,Peacock WJ,Dennis ES

    更新日期:2014-02-04 00:00:00

  • In vivo function of mutated spliced leader RNAs in Caenorhabditis elegans.

    abstract::The role of spliced leader RNA (SL RNA) in trans-splicing in Caenorhabditis elegans has been studied through a combination of in vitro mutagenesis and in vivo complementation of rrs-1 mutant nematodes, which lack endogenous SL1 RNA. Three classes of mutant SL1 RNAs have been found-those that rescue the lethal phenotyp...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.8.4235

    authors: Xie H,Hirsh D

    更新日期:1998-04-14 00:00:00

  • N-terminal domain of human Hsp90 triggers binding to the cochaperone p23.

    abstract::The molecular chaperone Hsp90 is a protein folding machine that is conserved from bacteria to man. Human, cytosolic Hsp90 is dedicated to folding of chiefly signal transduction components. The chaperoning mechanism of Hsp90 is controlled by ATP and various cochaperones, but is poorly understood and controversial. Here...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1011867108

    authors: Karagöz GE,Duarte AM,Ippel H,Uetrecht C,Sinnige T,van Rosmalen M,Hausmann J,Heck AJ,Boelens R,Rüdiger SG

    更新日期:2011-01-11 00:00:00

  • Oligomerization of opioid receptors with beta 2-adrenergic receptors: a role in trafficking and mitogen-activated protein kinase activation.

    abstract::G-protein-coupled receptors (GPCRs) have recently joined the list of cell surface receptors that dimerize. Dimerization has been shown to alter the ligand-binding, signaling, and trafficking properties of these receptors. Recent studies have shown that GPCRs heterodimerize with closely related members, resulting in th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.011384898

    authors: Jordan BA,Trapaidze N,Gomes I,Nivarthi R,Devi LA

    更新日期:2001-01-02 00:00:00

  • Spantide II, an effective tachykinin antagonist having high potency and negligible neurotoxicity.

    abstract::Spantide (D-Arg1-Pro2-Lys3-Pro4-Gln5-Gln6-D-Trp7-Phe8-D-Trp9-++ +Leu10-Leu11-NH2) was introduced as a tachykinin antagonist in 1984 and has served as a starting point in the design of new antagonists that have proven to be more effective and have exhibited no neurological side effects. The most remarkable and unpredic...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.12.4833

    authors: Folkers K,Feng DM,Asano N,Håkanson R,Weisenfeld-Hallin Z,Leander S

    更新日期:1990-06-01 00:00:00