Abstract:
:Fibronectin and alpha 1(I) and alpha 2(I) collagen proteins and RNAs are highly expressed during the growth phase of the non-transformed L6 alpha 1 rat myoblasts. When L6 alpha 1 cells from myotubes following transfer to low serum medium, the levels of fibronectin RNA decrease 8-fold, those of both alpha 2(I) transcripts decrease only 2-fold, while those of both alpha 1(I) transcripts remain stable. The L6 alpha 1 cell-derived non-differentiable low-malignant M4 cell and high-malignant RMS4 cell display only one size of alpha 1(I) and alpha 2(I) transcripts. Compared with L6 alpha 1 myoblasts, the levels of fibronectin and alpha 1(I) RNAs are reduced by factors of 4-5 and 9-10 respectively in both M4 and RMS4 and those of alpha 2(I) RNAs by factors of 10-11 and 20-22 in M4 and RMS4, respectively. Transcription rates are similarly decreased for fibronectin RNA, but are decreased less for collagen RNAs.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Leibovitch SA,Hillion J,Leibovitch MP,Guillier M,Schmitz A,Harel Jdoi
10.1016/0014-4827(86)90497-0subject
Has Abstractpub_date
1986-10-01 00:00:00pages
526-34issue
2eissn
0014-4827issn
1090-2422pii
0014-4827(86)90497-0journal_volume
166pub_type
杂志文章abstract::We present the first evidence that secretory organelle docking at the cell membrane can be reversed in vivo. In nondischarge (nd) mutants of Paramecium tetraurelia all trichocysts can be detached from the cell surface within 2-3 h by different means, including cytochalasin B (but not D), high cell density, or Ca2+ ion...
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journal_title:Experimental cell research
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