Histochemical demonstration of sodium-dependent glutamate uptake in brain tissues by glutamate dehydrogenase reaction.

Abstract:

:Using a special tetrazolium salt technique, a striking correlation was observed between Na+ concentration of the incubation medium and the formation of formazan catalyzed by glutamate dehydrogenase (GDH) in glutamatergic neuropil areas of the hippocampal formation, cerebellum, and other brain regions. Na+ concentrations of 130-150 mmol/l caused maximal formazan production. The GDH catalyzed sodium-dependent increase in formazan production is suggested to be a consequence of the sodium dependence of glutamate uptake in glutamatergic brain structures supplying the enzyme with substrate.

journal_name

Exp Brain Res

authors

Wolf G,Schünzel G,Rothe F

doi

10.1007/BF00236047

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

659-62

issue

3

eissn

0014-4819

issn

1432-1106

journal_volume

62

pub_type

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