Abstract:
AIMS:Pathology specimens often contain important margins that must be identified from gross examination of specimens through to microscopic examination. Commonly, unique colours of tissue-marking dye (TMD) are applied to each margin, which facilitates both macroscopic and microscopic identification. Various techniques have been described, but the colour endurance and fidelity of TMDs following special tissue processing have not been addressed. The aim of this study was to evaluate the performance of various TMDs through decalcification and immunohistochemistry (IHC) protocols. METHODS AND RESULTS:Samples of TMDs from two manufacturers and acrylic artists' inks were obtained in seven colours and applied to excess non-diagnostic surgical pathology tissue. Tissues were subjected to a decalcification protocol or directly processed in a routine fashion. The presence and colour of TMD or ink were assessed on routine H&E sections and following IHC. Of the colours that reliably survived routine processing, loss of colour and colour change following decalcification and IHC protocols were seen with one manufacturer's product. CONCLUSIONS:TMD may lose or change its colour during special tissue processing. This previously unreported artefact may lead to potentially serious errors in margin assessment and reporting. Laboratories should evaluate TMDs and inks through routine processing, decalcification, and IHC protocols, to ensure colour endurance and fidelity.
journal_name
Histopathologyjournal_title
Histopathologyauthors
Williams AS,Dakin Haché Kdoi
10.1111/his.12328subject
Has Abstractpub_date
2014-05-01 00:00:00pages
896-900issue
6eissn
0309-0167issn
1365-2559journal_volume
64pub_type
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