Abstract：
:A study was undertaken of the various factors affecting the recovery of heterotrophic bacteria from marine sediments. The dilution medium and culture medium were found to be of great importance in the recovery of heterotrophic colony forming units (CFU). Statistical analysis of the total viable counts obtained under the test conditions showed that artificial seawater (ASW) without further supplementation was equal to or superior to ASW plus 0.1% peptone or ASW plus 0.1% peptone and 0.1% glycerol. The addition of a surfactive agent, on the other hand, resulted in 95% inhibition of the recoverable CFU. The elapsed time (up to 12 hr) between recovery of a sedimentary core and completion of plating procedures was found to have little effect provided the sedimentary sample was removed from the core, placed in ASW, and stored in a refrigerator until actual plating occurred. It was further noted that lower organic nutrient concentrations, approximately one-tenth of those generally in use, resulted in significantly higher total viable counts. Finally, replicate contiguous sampling at three depths in a core resulted in no significant changes in the number of CFU from the surface samples, indicating a greater surface homogeneity than that previously suspected. The same pattern was not true, however, for samples obtained a lower positions in the core, thus indicating pockets of microbial concentration.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Litchfield CD,Rake JB,Zindulis J,Watanabe RT,Stein DJdoi
10.1007/BF02512390subject
Has Abstractpub_date
1974-12-01 00:00:00pages
219-33issue
1eissn
0095-3628issn
1432-184Xjournal_volume
1pub_type
杂志文章abstract：:The role of disturbance in structuring natural microbial communities has been largely unexplored. Disturbance associated with invertebrate ingestion can reduce bacterial biomass and alter metabolic activities and compositions of bacterial assemblages in marine sediments. The primary objectives of the research presente...
journal_title：Microbial ecology
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更新日期：1975-12-01 00:00:00
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journal_title：Microbial ecology
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journal_title：Microbial ecology
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journal_title：Microbial ecology
pub_type： 已发布勘误
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更新日期：2020-11-20 00:00:00
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更新日期：2015-01-01 00:00:00
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