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A novel approach for separating bacteriophages from other bacteriophages using affinity chromatography and phage display.

Abstract：

:Practical applications of bacteriophages in medicine and biotechnology induce a great need for technologies of phage purification. None of the popular methods offer solutions for separation of a phage from another similar phage. We used affinity chromatography combined with competitive phage display (i) to purify T4 bacteriophage from bacterial debris and (ii) to separate T4 from other contaminating bacteriophages. In 'competitive phage display' bacterial cells produced both wild types of the proteins (expression from the phage genome) and the protein fusions with affinity tags (expression from the expression vectors). Fusion proteins were competitively incorporated into the phage capsid. It allowed effective separation of T4 from a contaminating phage on standard affinity resins.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Ceglarek I,Piotrowicz A,Lecion D,Miernikiewicz P,Owczarek B,Hodyra K,Harhala M,Górski A,Dąbrowska K

doi

10.1038/srep03220

subject

Has Abstract

pub_date

2013-11-14 00:00:00

pages

3220

issn

2045-2322

pii

srep03220

journal_volume

3

pub_type

杂志文章

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