Phage T7 Gp2 inhibition of Escherichia coli RNA polymerase involves misappropriation of σ70 domain 1.1.

abstract：:An antibody (anti-p21ser) was raised against a ras p21-related synthetic peptide and was able to recognize specifically the substitution of serine for glycine at amino acid 12 of p21. This substitution causes oncogenic activation of p21. Anti-p21ser was found to immunoprecipitate v-Ki-ras p21 and to strongly inhibit i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark R,Wong G,Arnheim N,Nitecki D,McCormick F

更新日期：1985-08-01 00:00:00

abstract：:During mitosis, the mammalian Golgi vesiculates and, upon partitioning, reassembles in each daughter cell; however, it is not clear whether the disassembly process per se is important for partitioning or is merely an outcome of mitotic entry. Here, we show that Golgi vesiculation is required for progression to metapha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guizzunti G,Seemann J

更新日期：2016-10-25 00:00:00

abstract：:A surprising example of interspecies competition is the production by certain bacteria of hydrogen peroxide at concentrations that are lethal for others. A case in point is the displacement of Staphylococcus aureus by Streptococcus pneumoniae in the nasopharynx, which is of considerable clinical significance. How it i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Selva L,Viana D,Regev-Yochay G,Trzcinski K,Corpa JM,Lasa I,Novick RP,Penadés JR

更新日期：2009-01-27 00:00:00

abstract：:Although it is known that the human genome contains hundreds of microRNA (miRNA) genes and that each miRNA can regulate a large number of mRNA targets, the overall effect of miRNAs on mRNA tissue profiles has not been systematically elucidated. Here, we show that predicted human mRNA targets of several highly tissue-s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sood P,Krek A,Zavolan M,Macino G,Rajewsky N

更新日期：2006-02-21 00:00:00

abstract：:To investigate protein folding dynamics in terms of compactness, we developed a continuous-flow mixing device to make small-angle x-ray scattering measurements with the time resolution of 160 micros and characterized the radius of gyration (R(g)) of two folding intermediates of cytochrome c (cyt c). The early intermed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama S,Takahashi S,Kimura T,Ishimori K,Morishima I,Nishikawa Y,Fujisawa T

更新日期：2002-02-05 00:00:00

abstract：:Oncogene-induced senescence (OIS) is an initial barrier to tumor development. Reactive oxygen species (ROS) is critical for oncogenic Ras OIS, but the downstream effectors to mediate ROS signaling are still relatively elusive. Senescent cells develop a senescence-associated secretory phenotype (SASP). However, the mec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang P,Han L,Shen H,Wang P,Lv C,Zhao G,Niu J,Xue L,Wang QJ,Tong T,Chen J

更新日期：2014-05-27 00:00:00

abstract：:We studied the coupled binding and folding of alpha-synuclein, an intrinsically disordered protein linked with Parkinson's disease. Using single-molecule fluorescence resonance energy transfer and correlation methods, we directly probed protein membrane association, structural distributions, and dynamics. Results reve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferreon AC,Gambin Y,Lemke EA,Deniz AA

更新日期：2009-04-07 00:00:00

abstract：:Transcription factors (TFs) are primary regulators of gene expression in cells, where they bind specific genomic target sites to control transcription. Quantitative measurements of TF-DNA binding energies can improve the accuracy of predictions of TF occupancy and downstream gene expression in vivo and shed light on h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le DD,Shimko TC,Aditham AK,Keys AM,Longwell SA,Orenstein Y,Fordyce PM

更新日期：2018-04-17 00:00:00

abstract：:Global warming is expected to lead to a large increase in atmospheric water vapor content and to changes in the hydrological cycle, which include an intensification of precipitation extremes. The intensity of precipitation extremes is widely held to increase proportionately to the increase in atmospheric water vapor c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Gorman PA,Schneider T

更新日期：2009-09-01 00:00:00

abstract：:Argonaute (Ago) proteins are the key component of the RNA-induced silencing complex and mediate RNA interference (RNAi) in association with small RNAs. Although overall the mechanism of RNAi is well understood, many molecular details of this complex process are not. Here we report about in-depth steady-state and, in p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deerberg A,Willkomm S,Restle T

更新日期：2013-10-29 00:00:00

abstract：:Senescence is controlled by time-evolving networks that describe the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks for NAM/ATAF/CUC (NAC) transcription factors in Arabidopsis during leaf aging. The most evident characteristic of these time-dependent networks w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim HJ,Park JH,Kim J,Kim JJ,Hong S,Kim J,Kim JH,Woo HR,Hyeon C,Lim PO,Nam HG,Hwang D

更新日期：2018-05-22 00:00:00

abstract：:The x-ray structure of rubredoxin has been refined by energy minimization. The computed structure is constrained to have standard bond lengths, bond angles, and planar trans peptide groups. Also, since most of the steric overlaps have been relieved, it has a very low energy. As judged by the root mean square (RMS) dev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rasse D,Warme PK,Scheraga HA

更新日期：1974-09-01 00:00:00

abstract：:Persisting (P) cells (murine cells that resemble mast cells and grow continuously in vitro for prolonged periods in the presence of a specific growth factor) did not express detectable levels of Ia antigens (murine class II major histocompatibility antigens) when their growth was supported by partially purified P cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wong GH,Clark-Lewis I,McKimm-Breschkin JL,Schrader JW

更新日期：1982-11-01 00:00:00

abstract：:The full primary structure of the very potent opioid peptide dynorphin, from porcine pituitary, has been determined. It is (H)Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln(OH). The synthetic peptide with this sequence behaves identically to natural dynorphin in a number of ways, and it has the sa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldstein A,Fischli W,Lowney LI,Hunkapiller M,Hood L

更新日期：1981-11-01 00:00:00

abstract：:Activator-dependent recruitment of TFIID initiates formation of the transcriptional preinitiation complex. TFIID binds core promoter DNA elements and directs the assembly of other general transcription factors, leading to binding of RNA polymerase II and activation of RNA synthesis. How TATA box-binding protein (TBP) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wright KJ,Marr MT 2nd,Tjian R

更新日期：2006-08-15 00:00:00

abstract：:Antigenic variation of African trypanosomes results from the periodic activation of a single new variant cell surface glycoprotein (VSG) gene out of a repertoire of about a 1000 VSG genes. In spite of the apparently random genetic basis of the process of antigenic variation, the relapsing parasitemias are characterize...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agur Z,Abiri D,Van der Ploeg LH

更新日期：1989-12-01 00:00:00

abstract：:Poly(ADP ribose) polymerase inhibitors (PARPi) have efficacy in triple negative breast (TNBC) and ovarian cancers (OCs) harboring BRCA mutations, generating homologous recombination deficiencies (HRDs). DNA methyltransferase inhibitors (DNMTi) increase PARP trapping and reprogram the DNA damage response to generate HR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McLaughlin LJ,Stojanovic L,Kogan AA,Rutherford JL,Choi EY,Yen RC,Xia L,Zou Y,Lapidus RG,Baylin SB,Topper MJ,Rassool FV

更新日期：2020-07-28 00:00:00

abstract：:Although major research efforts have focused on how specific components of foodstuffs affect health, relatively little is known about a more fundamental aspect of diet, the frequency and circadian timing of meals, and potential benefits of intermittent periods with no or very low energy intakes. The most common eating...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mattson MP,Allison DB,Fontana L,Harvie M,Longo VD,Malaisse WJ,Mosley M,Notterpek L,Ravussin E,Scheer FA,Seyfried TN,Varady KA,Panda S

更新日期：2014-11-25 00:00:00

abstract：:Cerebellar granule cells are the most abundant neurons in the brain, and granule cell precursors (GCPs) are a common target of transformation in the pediatric brain tumor medulloblastoma. Proliferation of GCPs is regulated by the secreted signaling molecule Sonic hedgehog (Shh), but the mechanisms by which Shh control...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oliver TG,Grasfeder LL,Carroll AL,Kaiser C,Gillingham CL,Lin SM,Wickramasinghe R,Scott MP,Wechsler-Reya RJ

更新日期：2003-06-10 00:00:00

abstract：:Despite the recognized role of tumor necrosis factor (TNF) in inflammation and neuronal degeneration, anti-TNF therapeutics failed to treat neurodegenerative diseases. Animal disease models had revealed the antithetic effects of the two TNF receptors (TNFR) in the central nervous system, whereby TNFR1 has been associa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong Y,Fischer R,Naudé PJ,Maier O,Nyakas C,Duffey M,Van der Zee EA,Dekens D,Douwenga W,Herrmann A,Guenzi E,Kontermann RE,Pfizenmaier K,Eisel UL

更新日期：2016-10-25 00:00:00

abstract：:Circular RNA replicons have been reported in plants and, in one case, in animal cells. We describe such an element in yeast. In certain yeast strains, a 20S RNA species appears on transfer of cells to acetate medium. This phenotype shows cytoplasmic (non-Mendelian) inheritance and the 20S RNA is associated with 23-kDa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsumoto Y,Fishel R,Wickner RB

更新日期：1990-10-01 00:00:00

abstract：:We exploit the unusual genome organization of the ciliate cell to analyze the control of specific gene amplification during a nuclear differentiation process. Ciliates contain two types of nuclei within one cell, the macronucleus and the micronucleus; and after sexual reproduction a new macronucleus is formed from a m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heyse G,Jönsson F,Chang WJ,Lipps HJ

更新日期：2010-12-21 00:00:00

abstract：:DNA polymerases replicate DNA by adding nucleotides to a growing primer strand while avoiding frameshift and point mutations. Here we present a series of up to six successive replication events that were obtained by extension of a primed template directly in a crystal of the thermostable Bacillus DNA polymerase I. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnson SJ,Taylor JS,Beese LS

更新日期：2003-04-01 00:00:00

abstract：:Supported metal catalysts are extensively used in industrial and environmental applications. To improve their performance, it is crucial to identify the most active sites. This identification is, however, made challenging by the presence of a large number of potential surface structures that complicate such an assignm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang AC,Choksi T,Streibel V,Aljama H,Wrasman CJ,Roling LT,Goodman ED,Thomas D,Bare SR,Sánchez-Carrera RS,Schäfer A,Li Y,Abild-Pedersen F,Cargnello M

更新日期：2020-06-30 00:00:00

abstract：:The structure of cellulose microfibrils in wood is not known in detail, despite the abundance of cellulose in woody biomass and its importance for biology, energy, and engineering. The structure of the microfibrils of spruce wood cellulose was investigated using a range of spectroscopic methods coupled to small-angle ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernandes AN,Thomas LH,Altaner CM,Callow P,Forsyth VT,Apperley DC,Kennedy CJ,Jarvis MC

更新日期：2011-11-22 00:00:00

abstract：:Synaptic loss is the cardinal feature linking neuropathology to cognitive decline in Alzheimer's disease (AD). However, the mechanism of synaptic damage remains incompletely understood. Here, using FRET-based glutamate sensor imaging, we show that amyloid-β peptide (Aβ) engages α7 nicotinic acetylcholine receptors to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Talantova M,Sanz-Blasco S,Zhang X,Xia P,Akhtar MW,Okamoto S,Dziewczapolski G,Nakamura T,Cao G,Pratt AE,Kang YJ,Tu S,Molokanova E,McKercher SR,Hires SA,Sason H,Stouffer DG,Buczynski MW,Solomon JP,Michael S,Powers E

更新日期：2013-07-02 00:00:00

abstract：:We previously reported that KIF3A and KIF3B form a heterodimer that functions as a microtubule-based fast anterograde translocator of membranous organelles. We have also shown that this KIF3A/3B forms a complex with other associated polypeptides, named kinesin superfamily-associated protein 3 (KAP3). In the present st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamazaki H,Nakata T,Okada Y,Hirokawa N

更新日期：1996-08-06 00:00:00

abstract：:Based largely on studies in xenograft models, lipocalin-2 (Lcn2) has been implicated in the progression of multiple types of human tumors, including breast cancer. Here we examine the role of Lcn2 in mammary tumorigenesis and lung metastasis using an in vivo molecular genetics approach. We crossed a well-characterized...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berger T,Cheung CC,Elia AJ,Mak TW

更新日期：2010-02-16 00:00:00

abstract：:Selective autophagy of damaged mitochondria requires autophagy receptors optineurin (OPTN), NDP52 (CALCOCO2), TAX1BP1, and p62 (SQSTM1) linking ubiquitinated cargo to autophagic membranes. By using quantitative proteomics, we show that Tank-binding kinase 1 (TBK1) phosphorylates all four receptors on several autophagy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richter B,Sliter DA,Herhaus L,Stolz A,Wang C,Beli P,Zaffagnini G,Wild P,Martens S,Wagner SA,Youle RJ,Dikic I

更新日期：2016-04-12 00:00:00

abstract：:One of the strategies used by organisms to adapt to life under conditions of short energy supply is to use the by-product pyrophosphate to support cation gradients in membranes. Transport reactions are catalyzed by membrane-integral pyrophosphatases (PPases), which are classified into two homologous subfamilies: H(+)-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luoto HH,Baykov AA,Lahti R,Malinen AM

更新日期：2013-01-22 00:00:00