Bacterial production in a mesohumic lake estimated from [(14)C]leucine incorporation rate.

Abstract:

:Incorporation of [(14)C]leucine into proteins of bacteria was studied in a temperate mesohumic lake. The maximum incorporation of [(14)C] leucine was reached at a concentration of 30 nM determined in dilution cultures. Growth experiments were used to estimate factors for converting leucine incorporation to bacterial cell numbers or biomass. The initially high conversion factors calculated by the derivative method decreased to lower values after the bacteria started to grow. Average conversion factors were 7.09 × 10(16) cells mol(-1) and 7.71 × 10(15) μm(3) mol(-1), if the high initial values were excluded. Using the cumulative method, the average conversion factor was 5.38 × 10(15) μm(-3) mol(-1) I . The empirically measured factor converting bacterial biomass to carbon was 0.36 pg C μm(-3) or 33.1 fg C cell(-1). Bacterial production was highest during the growing season, ranging between 1.8 and 13.2 μg C liter(-1) day(-1), and lowest in winter, at 0.2-2.9 μg C liter(-1) day(-1). Bacterial production showed clear response to changes in the phytoplankton production, which indicates that photosynthetically produced dissolved compounds were used by bacteria. In the epilimnion bacterial production was, on average, 19-33% of primary production. Assuming 50% growth efficiency for bacteria, the allochthonous organic carbon could have also been an additional energy and carbon source for bacteria, especially in autumn and winter. In winter, a strong relationship was found between temperature and bacterial production. The measuring of [(14)C]leucine incorporation proved to be a simple and useful method for estimating bacterial production in humic water. However, an appropriate amount of [(14)C]leucine has to be used to ensure the maximum uptake of label and to minimize isotope dilution.

journal_name

Microb Ecol

journal_title

Microbial ecology

authors

Tulonen T

doi

10.1007/BF00176953

subject

Has Abstract

pub_date

1993-11-01 00:00:00

pages

201-17

issue

3

eissn

0095-3628

issn

1432-184X

journal_volume

26

pub_type

杂志文章
  • Nutrient and temperature limitation of bacterioplankton growth in temperate lakes.

    abstract::Limitation of bacterioplankton production by nutrients and temperature was investigated in eight temperate lakes in summer. Six of the lakes were resampled in autumn. The lakes differ in nutrient content, water color, and concentration of dissolved organic carbon. Nutrients (phosphorus, nitrogen, and organic carbon) w...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-004-0259-4

    authors: Vrede K

    更新日期:2005-02-01 00:00:00

  • Temporal and spatial patterns of Bartonella infection in black-tailed prairie dogs (Cynomys ludovicianus).

    abstract::We describe the temporal dynamics and spatial distribution of Bartonella in black-tailed prairie dogs (Cynomys ludovicianus) based on a longitudinal study conducted in 20 black-tailed prairie dog (BTPD) colonies in Boulder County, CO from 2003 to 2005. Bartonella infection was widely distributed in all colonies with a...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-007-9355-6

    authors: Bai Y,Kosoy MY,Ray C,Brinkerhoff RJ,Collinge SK

    更新日期:2008-08-01 00:00:00

  • Response of benthic protozoa and thraustochytrid protists to fish farm impact in seagrass (Posidonia oceanica) and soft-bottom sediments.

    abstract::We investigated the impact of fish farm biodeposition on benthic bacteria, thraustochytrid protists, and heterotrophic protozoa (nanoflagellates and ciliates) in an oligotrophic area of the Mediterranean Sea. The fish farm impact was investigated both on a seagrass (Posidonia oceanica) bed and on soft bottom sediments...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-004-0117-4

    authors: Bongiorni L,Mirto S,Pusceddu A,Danovaro R

    更新日期:2005-08-01 00:00:00

  • Abundance, diversity, and dynamics of viruses on microorganisms in activated sludge processes.

    abstract::We examined the abundance of viruses on microorganisms in activated sludge and the dynamics of their community structure. Direct counting with epifluorescence microscopy and pulsed-field gel electrophoresis (PFGE) were applied to 20 samples from 14 full-scale wastewater treatment plants (wwtps) treating municipal, ind...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-006-9150-9

    authors: Otawa K,Lee SH,Yamazoe A,Onuki M,Satoh H,Mino T

    更新日期:2007-01-01 00:00:00

  • Drying-rewetting cycles affect fungal and bacterial growth differently in an arable soil.

    abstract::Drying and rewetting is a frequent physiological stress for soil microbial communities; a stress that is predicted to grow more influential with future climate change. We investigated the effect of repeated drying-rewetting cycles on bacterial (leucine incorporation) and fungal (acetate in ergosterol incorporation) gr...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-010-9723-5

    authors: Bapiri A,Bååth E,Rousk J

    更新日期:2010-08-01 00:00:00

  • Effects of Agaricus lilaceps fairy rings on soil aggregation and microbial community structure in relation to growth stimulation of western wheatgrass (Pascopyrum smithii) in Eastern Montana rangeland.

    abstract::Stimulation of plant productivity caused by Agaricus fairy rings has been reported, but little is known about the effects of these fungi on soil aggregation and the microbial community structure, particularly the communities that can bind soil particles. We studied three concentric zones of Agaricus lilaceps fairy rin...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-013-0194-3

    authors: Caesar-Tonthat TC,Espeland E,Caesar AJ,Sainju UM,Lartey RT,Gaskin JF

    更新日期:2013-07-01 00:00:00

  • Species diversity, distribution, and genetic structure of endophytic and epiphytic Trichoderma associated with banana roots.

    abstract::Selective isolation, molecular identification and AFLP were used to investigate the distribution of the various species of endophytic and epiphytic Trichoderma associated with banana roots and to compare and contrast their genetic structure. Three specific groups of Trichoderma were observed in the roots of banana. Gr...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-010-9770-y

    authors: Xia X,Lie TK,Qian X,Zheng Z,Huang Y,Shen Y

    更新日期:2011-04-01 00:00:00

  • Food web structure in the recently flooded Sep Reservoir as inferred from phytoplankton population dynamics and living microbial biomass.

    abstract::Phytoplankton dynamics, bacterial standing stocks and living microbial biomass (derived from ATP measurements, 0.7-200 mm size class) were examined in 1996 in the newly flooded (1995) Sep Reservoir ('Massif Central,' France), for evidence of the importance of the microbial food web relative to the traditional food cha...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-001-1015-7

    authors: Tadonléké RD,Jugnia LB,Sime-Ngando T,Devaux J,Romagoux JC

    更新日期:2002-01-01 00:00:00

  • The biogeography of ammonia-oxidizing bacterial communities in soil.

    abstract::Although ammonia-oxidizing bacteria (AOB) are likely to play a key role in the soil nitrogen cycle, we have only a limited understanding of how the diversity and composition of soil AOB communities change across ecosystem types. We examined 23 soils collected from across North America and used sequence-based analyses ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-009-9517-9

    authors: Fierer N,Carney KM,Horner-Devine MC,Megonigal JP

    更新日期:2009-08-01 00:00:00

  • Analysis of spatial and temporal variation in the community structure of yeasts associated with decayingOpuntia cactus.

    abstract::The microbial structure within, between, and over time in decaying cladodes of the common prickly pearOpuntia stricta was studied at each of two separate localities. In general, the effective number of yeast species and yeast species diversity increased as the rot aged to the observed maximum time of 4 weeks. Yeast he...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02012946

    authors: Barker JS,Starmer WT,Vacek DC

    更新日期:1987-11-01 00:00:00

  • Effect of culture media onLactobacillus hydrophobicity and electrophoretic mobility.

    abstract::The hydrophobicity of six strains representing three species ofLactobacillus was measured using dextran-polyethylene glycol contact angle measurements. These ranged from 123.6° forLactobacillus casei douche to 26.2° forL. casei RC-17 under identical growth conditions. The results indicated that the nutritional environ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02025590

    authors: Eisen A,Reid G

    更新日期:1989-01-01 00:00:00

  • Effects of Agricultural Chemicals on DNA Sequence Diversity of Soil Microbial Community: A Study with RAPD Marker.

    abstract::The DNA sequence diversities for microbial communities in four soils affected by agricultural chemicals (mainly triadimefon and ammonium bicarbonate and their intermediates) were evaluated by Random Amplified Polymorphic DNA (RAPD) analysis. Fourteen random primers were used to amplify RAPDs from four soil microbial c...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s002489900180

    authors: Yang Y,Yao J,Hu S,Qi Y

    更新日期:2000-01-01 00:00:00

  • Ecology of stem-nodulating Rhizobium and Azorhizobium in four vegetation zones of Senegal.

    abstract::The occurrence and distribution of Azorhizobium and Rhizobium strains that induce stem nodulation of Sesbania rostrata were determined in four vegetation zones in Senegal. Based on tests with 16 Rhizobium and 10 Azorhizobium strains nodulating S. rostrata, a method was devised to distinguish among the strains. In all ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF00217424

    authors: Robertson BK,Dreyfus B,Alexander M

    更新日期:1995-01-01 00:00:00

  • Microbial community succession in an unvegetated, recently deglaciated soil.

    abstract::Primary succession is a fundamental process in macroecosystems; however, if and how soil development influences microbial community structure is poorly understood. Thus, we investigated changes in the bacterial community along a chronosequence of three unvegetated, early successional soils ( approximately 20-year age ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-006-9144-7

    authors: Nemergut DR,Anderson SP,Cleveland CC,Martin AP,Miller AE,Seimon A,Schmidt SK

    更新日期:2007-01-01 00:00:00

  • Biotic disturbance, recolonization, and early succession of bacterial assemblages in intertidal sediments.

    abstract::The role of disturbance in structuring natural microbial communities has been largely unexplored. Disturbance associated with invertebrate ingestion can reduce bacterial biomass and alter metabolic activities and compositions of bacterial assemblages in marine sediments. The primary objectives of the research presente...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-003-1031-x

    authors: Plante CJ,Wilde SB

    更新日期:2004-08-01 00:00:00

  • Dysbiosis signature of fecal microbiota in colorectal cancer patients.

    abstract::The human gut microbiota is a complex system that is essential to the health of the host. Increasing evidence suggests that the gut microbiota may play an important role in the pathogenesis of colorectal cancer (CRC). In this study, we used pyrosequencing of the 16S rRNA gene V3 region to characterize the fecal microb...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-013-0245-9

    authors: Wu N,Yang X,Zhang R,Li J,Xiao X,Hu Y,Chen Y,Yang F,Lu N,Wang Z,Luan C,Liu Y,Wang B,Xiang C,Wang Y,Zhao F,Gao GF,Wang S,Li L,Zhang H,Zhu B

    更新日期:2013-08-01 00:00:00

  • Decomposition of Cr(V)-diols to Cr(III) complexes by Arthrobacter oxydans.

    abstract::We demonstrated previously that Cr(VI) is readily reduced to oxoCr(V)-diols at the surface of Arthrobacter oxydans-a Gram-positive aerobic bacteria isolated from Columbia basalt rocks originated from a highly contaminated site in the USA. Here, we report an electron spin resonance (ESR) study of Cr(III) hydroxide form...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-008-9476-6

    authors: Tsibakhashvili NY,Kalabegishvili TL,Rcheulishvili AN,Murusidze IG,Rcheulishvili OA,Kerkenjia SM,Holman HY

    更新日期:2009-02-01 00:00:00

  • Response of ammonia oxidizing bacteria and archaea to acute zinc stress and different moisture regimes in soil.

    abstract::Ammonia oxidation has been intensively studied for its sensitivity to environmental shifts and stresses. However, acute stress effects on the occurrence and composition of ammonia oxidizing bacteria (AOB) and archaea (AOA) based on expression of related molecular markers in complex soil environments have been to an ex...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-012-0081-3

    authors: Vasileiadis S,Coppolecchia D,Puglisi E,Balloi A,Mapelli F,Hamon RE,Daffonchio D,Trevisan M

    更新日期:2012-11-01 00:00:00

  • Response of induced perturbation on replicating β-proteobacterial ammonia-oxidizing populations in soil.

    abstract::The short-term response of induced perturbation by 4-ethylphenol on β-proteobacterial ammonia oxidizers (β-AOB) was investigated in two soils with initial differences in community structure. The hypotheses were that short-term effects of a disturbance of the AOB community is best monitored by specifically looking at t...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-011-9970-0

    authors: Nyberg KA,Enwall K,Schnürer A,Sundh I,Hallin S

    更新日期:2012-04-01 00:00:00

  • An Insight into the Secondary Metabolism of Muscodor yucatanensis: Small-Molecule Epigenetic Modifiers Induce Expression of Secondary Metabolism-Related Genes and Production of New Metabolites in the Endophyte.

    abstract::Muscodor spp. are proficient producers of bioactive volatile organic compounds (VOCs) with many potential applications. However, all members of this genus produce varying amounts and types of VOCs which suggests the involvement of epigenetics as a possible explanation. The members of this genus are poorly explored for...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-016-0901-y

    authors: Qadri M,Nalli Y,Jain SK,Chaubey A,Ali A,Strobel GA,Vishwakarma RA,Riyaz-Ul-Hassan S

    更新日期:2017-05-01 00:00:00

  • Distribution of exopolymeric substances in the littoral sediments of an oligotrophic lake.

    abstract::Bacteria and algae release exopolymeric substances (EPS) that perform a wide range of important functions in aquatic and terrestrial systems. In this study we measured EPS in sediments at nine littoral sites around a shallow oligotrophic basin, and tested whether the concentration and composition of EPS was related to...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-002-1064-6

    authors: Hirst CN,Cyr H,Jordan IA

    更新日期:2003-07-01 00:00:00

  • Soil Inoculation with Bacillus spp. Modifies Root Endophytic Bacterial Diversity, Evenness, and Community Composition in a Context-Specific Manner.

    abstract::The use of microbial inoculants containing plant growth-promoting rhizobacteria as a promoter of plant fitness and health is becoming increasingly popular in agriculture. However, whether and how these bacteria affect indigenous bacterial communities in field conditions is sparsely explored. We studied the effects of ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-018-1160-x

    authors: Gadhave KR,Devlin PF,Ebertz A,Ross A,Gange AC

    更新日期:2018-10-01 00:00:00

  • Control of Hydraulic Load on Bacterioplankton Diversity in Cascade Hydropower Reservoirs, Southwest China.

    abstract::Hydroelectric reservoirs are highly regulated ecosystems, where the understanding on bacterioplankton has been very limited so far. In view of significant changes in river hydrological conditions by dam construction, hydraulic load (i.e., the ratio of mean water depth to water retention time) was assumed to control ba...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-020-01523-8

    authors: Yang M,Shi J,Wang B,Xiao J,Li W,Liu CQ

    更新日期:2020-10-01 00:00:00

  • Importance of Bacterial Maintenance Respiration in a Subarctic Estuary: a Proof of Concept from the Field.

    abstract::Bacterial respiration contributes to atmospheric carbon dioxide accumulation and development of hypoxia and is a critical, often overlooked, component of ecosystem function. This study investigates the concept that maintenance respiration is a significant proportion of bacterial respiration at natural nutrient levels ...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-018-1244-7

    authors: Vikström K,Wikner J

    更新日期:2019-04-01 00:00:00

  • Fungi from Admiralty Bay (King George Island, Antarctica) Soils and Marine Sediments.

    abstract::Extreme environments such as the Antarctic can lead to the discovery of new microbial taxa, as well as to new microbial-derived natural products. Considering that little is known yet about the diversity and the genetic resources present in these habitats, the main objective of this study was to evaluate the fungal com...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-018-1217-x

    authors: Wentzel LCP,Inforsato FJ,Montoya QV,Rossin BG,Nascimento NR,Rodrigues A,Sette LD

    更新日期:2019-01-01 00:00:00

  • Fungal growth and biomass development is boosted by plants in snow-covered soil.

    abstract::Soil microbial communities follow distinct seasonal cycles which result in drastic changes in processes involving soil nutrient availability. The biomass of fungi has been reported to be highest during winter, but is fungal growth really occurring in frozen soil? And what is the effect of plant cover on biomass format...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/s00248-011-0001-y

    authors: Kuhnert R,Oberkofler I,Peintner U

    更新日期:2012-07-01 00:00:00

  • The significance of viruses to mortality in aquatic microbial communities.

    abstract::A variety of approaches including enumeration of visibly infected microbes, removal of viral particles, decay of viral infectivity, and measurements of viral production rates have been used to infer the impact of viruses on microbial mortality. The results are surprisingly consistent and suggest that, on average, abou...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF00166813

    authors: Suttle CA

    更新日期:1994-09-01 00:00:00

  • Protein Profile Variation in Cultivated and Native Freshwater Microorganisms Exposed to Chemical Environmental Pollutants

    abstract::Assimilation of 35S-precursors into microbial proteins was used to investigate toxicity and adaptational responses that occur in nutrient enriched and natural freshwater samples experimentally contaminated with benzene, toluene, trichloroethylene (TCE), or xylene. Experiments were conducted to analyze (1) the potentia...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF00171573

    authors: Ogunseitan OA

    更新日期:1996-05-01 00:00:00

  • Analysis of the community structure of yeasts associated with the decaying stems of cactus. I.Stenocereus gummosus.

    abstract::Yeast communities of decayingStenocereus gummosus were analyzed for spatial, temporal, and physiological characteristics. Analysis of random samples within plants, between plants, and between localities shows that the species proportions of the yeast community are relatively constant within plants and between localiti...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02011463

    authors: Starmer WT

    更新日期:1982-06-01 00:00:00

  • Effects of a clay mineral on microbial predation and parasitism ofEscherichia coli.

    abstract::Montmorillonitic clay influences the biological control ofEscherichia coli in aquatic systems, the magnitude of the effects being dependent on the state of the clay and the type of host-antagonist interaction. The interaction ofBdellovibrio andE. coli was partially inhibited by the presence of montmorillonite. Because...

    journal_title:Microbial ecology

    pub_type: 杂志文章

    doi:10.1007/BF02013272

    authors: Roper MM,Marshall KC

    更新日期:1977-12-01 00:00:00