Formaldehyde induces micronuclei in mouse erythropoietic cells and suppresses the expansion of human erythroid progenitor cells.


:Although formaldehyde (FA) has been classified as a human leukemogen, the mechanisms of leukemogenesis remain elusive. Previously, using colony-forming assays in semi-solid media, we showed that FA exposure in vivo and in vitro was toxic to human hematopoietic stem/progenitor cells. In the present study, we have applied new liquid in vitro erythroid expansion systems to further investigate the toxic effects of FA (0-150 μM) on cultured mouse and human hematopoietic stem/progenitor cells. We determined micronucleus (MN) levels in polychromatic erythrocytes (PCEs) differentiated from mouse bone marrow. We measured cell growth, cell cycle distribution, and chromosomal instability, in erythroid progenitor cells (EPCs) expanded from human peripheral blood mononuclear cells. FA significantly induced MN in mouse PCEs and suppressed human EPC expansion in a dose-dependent manner, compared with untreated controls. In the expanded human EPCs, FA slightly increased the proportion of cells in G2/M at 100 μM and aneuploidy frequency in chromosomes 7 and 8 at 50 μM. Our findings provide further evidence of the toxicity of FA to hematopoietic stem/progenitor cells and support the biological plausibility of FA-induced leukemogenesis.


Toxicol Lett


Toxicology letters


Ji Z,Li X,Fromowitz M,Mutter-Rottmayer E,Tung J,Smith MT,Zhang L




Has Abstract


2014-01-13 00:00:00














  • Experimental exposure to 1,1,1-trifluoroethane (HFC-143a): uptake, disposition and acute effects in male volunteers.

    abstract::The aim of this study was to determine the toxicokinetics and some effects of 1,1,1-trifluoroethane (HFC-143a) in humans. Nine male volunteers were experimentally exposed to 500ppm HFC-143a for 2h during light physical exercise (50W) in an exposure chamber. Blood, urine and exhaled air were sampled before, during and ...

    journal_title:Toxicology letters

    pub_type: 临床试验,杂志文章


    authors: Gunnare S,Ernstgård L,Sjögren B,Johanson G

    更新日期:2007-08-01 00:00:00

  • Comparison of the toxicity of methylcyclopentadienyl manganese tricarbonyl with that of its two major metabolites.

    abstract::Pneumotoxicity and lethality resulting from administration of methylcyclopentadienyl manganese tricarbonyl (MMT) and its 2 major metabolites in rats were compared. Following i.p. injection, MMT was found to have an LD50 value of 12.1 mg/kg. Neither of the metabolites appeared to have significant acute toxicity even wh...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Cox DN,Traiger GJ,Jacober SP,Hanzlik RP

    更新日期:1987-11-01 00:00:00

  • Coumarin derivatives, but not coumarin itself, cause skin irritation via topical delivery.

    abstract::Coumarin and its derivatives are widely employed as a fragrance in cosmetics and skin care products. The skin absorption level and possible disruption to the skin by topical application of coumarins were evaluated in this study. Percutaneous absorption of osthole, daphnoretin, coumarin, byakangelicin, and 7-hydroxycou...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Pan TL,Wang PW,Aljuffali IA,Leu YL,Hung YY,Fang JY

    更新日期:2014-04-21 00:00:00

  • Cardiomyopathy in Soman and Sarin intoxicated rats.

    abstract::Rats surviving various single dose of the organophosphorus anticholinesterase nerve agents Soman and Sarin were examined by light microscopy at intervals up to 35 days post-exposure. Brain lesions, identical to those that have been reported elsewhere were present, as well as a previously unreported finding associated ...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Singer AW,Jaax NK,Graham JS,McLeod CG Jr

    更新日期:1987-05-01 00:00:00

  • The tobacco carcinogen NNK disturbs mitotic chromosome alignment by interrupting p53 targeting to the centrosome.

    abstract::The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is the most potent risk factor among tobacco-related carcinogens in lung cancer progression and outcomes. Although genetic mutations and chromosome instability have been detected in NNK-induced lung tumors, the oncogenic mechanisms o...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Park JE,Jang YL,Jang CY

    更新日期:2017-11-05 00:00:00

  • Anti-apoptotic effect of hepatocyte growth factor from actinomycin D in hepatocyte-derived HL7702 cells is associated with activation of PI3K/Akt signaling.

    abstract::Actinomycin D (ActD) is a well-known cytotoxic chemotherapeutic reagent and the prevention of ActD-induced apoptotic cell death has been an attractive issue for biomedical investigators. Since phosphatidylinositol-3 kinase (PI3K)/Akt pathway is essential for cell survival, the present study has examined whether the pr...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Li W,Cai S,Cai L,Li X

    更新日期:2006-08-20 00:00:00

  • An in vitro investigation on the cytotoxic and nuclear receptor transcriptional activity of the mycotoxins fumonisin B1 and beauvericin.

    abstract::Fumonisin B1 (FB1) and beauvericin (BEA) are secondary metabolites of filamentous fungi, which under appropriate temperature and humidity conditions may develop on various foods and feeds. To date few studies have been performed to evaluate the toxicological and endocrine disrupting effects of FB1 and BEA. The present...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Fernández-Blanco C,Frizzell C,Shannon M,Ruiz MJ,Connolly L

    更新日期:2016-08-22 00:00:00

  • A dual effect of some 5-HT3 receptor antagonists on cisplatin-induced emesis in the pigeon.

    abstract::In the present study, the emetic effect of the anticancer drug cisplatin, and protective effects of 5-HT3 receptor antagonists against cisplatin emesis were investigated in the pigeon. The experimental setting involved the i.v. administration of drugs and subsequent observation of the percentage of vomiting animals an...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Navarra P,Martire M,del Carmine R,Pozzoli G,Preziosi P

    更新日期:1992-12-01 00:00:00

  • Genotoxicity--threshold or not? Introduction of cases of industrial chemicals.

    abstract::Many industrially and environmentally important industrial carcinogens display effects that lead them to be viewed and regulated as 'genotoxic compounds'. Some of these chemicals cause experimental tumours only at high or toxic doses. The current view is that non-threshold principles should be applied for risk assessm...

    journal_title:Toxicology letters

    pub_type: 杂志文章,评审


    authors: Bolt HM

    更新日期:2003-04-11 00:00:00

  • Manganese-induced oxidative DNA damage in neuronal SH-SY5Y cells: attenuation of thymine base lesions by glutathione and N-acetylcysteine.

    abstract::Manganese (Mn) is an essential trace element required for normal function and development. However, exposure to this metal at elevated levels may cause manganism, a progressive neurodegenerative disorder with neurological symptoms similar to idiopathic Parkinson's disease (IPD). Elevated body burdens of Mn from exposu...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Stephenson AP,Schneider JA,Nelson BC,Atha DH,Jain A,Soliman KF,Aschner M,Mazzio E,Renee Reams R

    更新日期:2013-04-26 00:00:00

  • Absence of tumor promoting activity of Euphorbia milii latex on the mouse back skin.

    abstract::Euphorbia milii (Euphorbiaceae) is a decorative plant used in gardens and living fences. In China, it has also been employed in herbal remedies for hepatitis and abdominal edema. Since E. milii latex--lyophilized or in natura--proved to be a potent plant molluscicide, its toxicity to non-target organisms has been comp...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Delgado IF,De-Carvalho RR,De-Oliveira AC,Kuriyama SN,Oliveira-Filho EC,Souza CA,Paumgartten FJ

    更新日期:2003-11-30 00:00:00

  • Therapeutic response to single intravenous bolus administration of formate dehydrogenase in methanol-intoxicated rats.

    abstract::Methanol remains to be a major public and environmental health hazard. Formic acid is the toxic metabolite responsible for the metabolic acidosis observed in methanol poisoning in humans, in non-human primates and in folate-depleted rodents. Cytochrome oxidase inhibition by formate leads to lactic acid accumulation, w...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Muthuvel A,Rajamani R,Sheeladevi R

    更新日期:2006-02-20 00:00:00

  • Synergistic effects of peroxynitrite on arecoline-induced cytotoxicity in human buccal mucosal fibroblasts.

    abstract::Epidemiological studies have demonstrated a clear association between betel nut chewing and an increased risk for oral mucosal lesions. Arecoline, the most abundant betel alkaloid, is considered the most important etiologic factor in betel nuts. In addition, most betel nut chewers are also smokers. In order to elucida...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Chang YC,Tai KW,Chou MY,Tseng TH

    更新日期:2000-12-20 00:00:00

  • Mutagenic effects of fluphenazine hydrochloride in Drosophila melanogaster.

    abstract::The mutagenic potential of fluphenazine hydrochloride was tested on male germ cells of Drosophila melanogaster. The criterion used was sex-linked recessive lethal mutations. Oregon-K males of D. melanogaster, reared on a medium containing 0.01, 0.02 and 0.03 mg/ml of the drug, were screened for sex-linked recessive le...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Rao KP,Rao MS

    更新日期:1980-12-01 00:00:00

  • Genotoxicity of the isoflavones genistein, daidzein and equol in V79 cells.

    abstract::Hormonally active chemicals in the human diet, such as man-made estrogenic chemicals or plant-derived compounds (phytoestrogens), have become a matter of public concern. A significant part of human exposure to phytoestrogens is attributable to soy isoflavones. Besides their estrogenic properties, soy isoflavones also ...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Di Virgilio AL,Iwami K,Wätjen W,Kahl R,Degen GH

    更新日期:2004-06-15 00:00:00

  • Acute nephrotoxicity induced by N-(3,5-dichlorophenyl)-3-hydroxysuccinamic acid in Fischer 344 rats.

    abstract::N-(3,5-Dichlorophenyl)succinimide (NDPS) induces nephrotoxicity via one or more metabolites which arise from oxidation of the succinimide ring. The purpose of this study was to examine the nephrotoxic potential of N-(3,5-dichlorophenyl)-3-hydroxysuccinamic acid (3-NDHSA), a potential metabolite of NDPS and a positiona...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Rankin GO,Shih H,Teets VJ,Nicoll DW,Brown PI

    更新日期:1989-09-01 00:00:00

  • Endoplasmic reticulum stress, an important factor in the development of Parkinson's disease.

    abstract::Similar to other types of neuronal degeneration, Parkinson's disease (PD) is characterized by the aggregation of a pathological protein, α-synuclein. The endoplasmic reticulum (ER) is the principal site of protein synthesis, quality control and degradation. Genetic mutants, environmental insults and other factors dist...

    journal_title:Toxicology letters

    pub_type: 杂志文章,评审


    authors: Mou Z,Yuan YH,Zhang Z,Song LK,Chen NH

    更新日期:2020-05-15 00:00:00

  • Quantitative perspectives on behavioral toxicology.

    abstract::Behavioral endpoints offer special challenges to toxicology. One arises from the sometimes conflicting ways in which adverse effects may be expressed. For example, both increases and decreases in response rates of schedule-controlled performance may be produced by a specific agent, depending on the specific schedule. ...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Weiss B

    更新日期:1988-10-01 00:00:00

  • A new physiological biomarker for nitrate exposure in humans.

    abstract::After toxicological studies with nitrate/nitrite in rats it was observed with nuclear magnetic resonance that N-methylnicotinamide (NMN), a metabolite of tryptophan was increased. The use of NMN as a biomarker for nitrate/nitrite exposure was investigated further in additional experiments with rats and in a human stud...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Jansen EH,van den Berg RH,Boink AB,Hegger C,Meulenbelt J

    更新日期:1995-05-01 00:00:00

  • The therapeutic effects of Longikaurin A, a natural ent-kauranoid, in esophageal squamous cell carcinoma depend on ROS accumulation and JNK/p38 MAPK activation.

    abstract::Effective treatments for esophageal squamous cell carcinoma (ESCC), one of the most common cancers in China, are lacking. Longikaurin A (LK-A), an ent-kauranoid diterpenoid isolated from Isodon ternifolius, has been shown to have potent cytotoxic effects on ESCC cells both in vivo and in vitro, mainly by inducing apop...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Che Y,Wang J,Yuan Z,Li Y,Lu Z,Zhang Z,Zhang J,Wan J,Sun H,Chen Z,Pu J,He J

    更新日期:2017-10-05 00:00:00

  • Thirteen-week subchronic toxicity study of thiamphenicol in F344 rats.

    abstract::A 13-week subchronic toxicity study of thiamphenicol (TAP) was performed in F344 rats. The minimum lethal dose was estimated to be greater than 10 g/kg body weight, when a single dose of 4-10 g/kg of TAP was given orally. In the subchronic toxicity study, groups of 12 F344 rats of each sex were given solutions contain...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Ando J,Ishihara R,Imai S,Takano S,Kitamura T,Takahashi M,Yoshida M,Maekawa A

    更新日期:1997-04-28 00:00:00

  • Elimination pattern and biodistribution of [14C]chlorferron in rats.

    abstract::Administration of [14C]chlorferron in a single oral dose of 0.5 and 20 mg/kg body weight to female rats resulted in a urinary excretion of greater than 74% of the given dose during the first 24 h. Approx. 8% of the dose was eliminated in faeces within 7 days. 7 days after dosing, very low levels of [14C]chlorferron-de...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Malik JK,Lay JP,Klein W,Korte F

    更新日期:1981-03-01 00:00:00

  • Toxicokinetics and toxicodynamics of iron poisoning.

    abstract::Iron is a unique poison because it is not a xenobiotic. It is an essential element and highly reactive. Because of the critical dependence upon iron and its potential to damage tissues, elaborate mechanisms have evolved for its efficient absorption, transport, cellular uptake, storage and conservation. These are incom...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Tenenbein M

    更新日期:1998-12-28 00:00:00

  • Correlation between the DNA-binding affinity of topoisomerase inhibiting drugs and their capacity to induce hematopoetic cell differentiation.

    abstract::There is accumulating evidence that both type I and type II DNA-topoisomerases play a key role in cellular differentiation. Human HL-60 leukemia cells can be induced to monocytic or granulocytic differentiation with various compounds; amongst them camptothecin, a topoisomerase I inhibitor and VP-16, VM-26 and mitoxant...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Gieseler F,Boege F,Clark M,Meyer P

    更新日期:1993-04-01 00:00:00

  • Currently available in vitro methods used in the regulatory toxicology.

    abstract::About two decades ago in vitro mutagenicity tests were adopted as the first in vitro methods in regulatory toxicology. For reasons of animal welfare and better science, many validation studies of various in vitro methods were performed between 1985 and 1995 for their potential to replace the Draize eye irritation test...

    journal_title:Toxicology letters

    pub_type: 杂志文章,评审


    authors: Liebsch M,Spielmann H

    更新日期:2002-02-28 00:00:00

  • Induction of oxidative stress by endosulfan and protective effect of lipid-soluble antioxidants against endosulfan-induced oxidative damage.

    abstract::The toxic mechanism of endosulfan, a widely used organochlorine pesticide, was investigated in Saccharomyces cerevisiae and human cell lines. A concentration-dependent inhibition of cell growth was observed when S. cerevisiae was exposed to endosulfan, and its cytotoxicity (IC(50)) was found to be 49 microM and 86 mic...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Sohn HY,Kwon CS,Kwon GS,Lee JB,Kim E

    更新日期:2004-07-15 00:00:00

  • General 4-week toxicity study with EMS in the rat.

    abstract::In this subacute toxicity study, ethyl methanesulfonate (EMS) was administered daily by oral gavage to SPF-bred Wistar rats of both sexes at dose levels of 20, 60 and 180/120 mg/kg body weight (bw)/day for a period of 28 days (for 19 days in the high-dose group). A control group was treated similarly with the vehicle,...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Pfister T,Eichinger-Chapelon A

    更新日期:2009-11-12 00:00:00

  • Indirect human exposure to pharmaceuticals via drinking water.

    abstract::There are numerous observations of pharmaceuticals (or their metabolites) as contaminants in wastewater, surface water and groundwater. This implies a potential for indirect human exposure to pharmaceuticals via drinking water supplies. Various effect benchmarks may be employed in an evaluation of the significance of ...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Webb S,Ternes T,Gibert M,Olejniczak K

    更新日期:2003-05-15 00:00:00

  • T(H1)-directed modulation of in vitro cytokine production in human peripheral blood mononuclear cells by styrene-7.8-oxide.

    abstract::Styrene and its chiral main metabolite styrene-7.8-oxide are well characterized regarding their cytotoxic, genotoxic and neurotoxic properties. To our knowledge, no data exist on the influence of styrene and styrene-7.8-oxide on immune reactions. Epidemiological studies suggest that exposure to environmental pollutant...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Merker GH,Metzner G,Raabe F

    更新日期:2006-01-05 00:00:00

  • Estrogenic activity of environmental polycyclic aromatic hydrocarbons in uterus of immature Wistar rats.

    abstract::Polycyclic aromatic hydrocarbons (PAHs) are an important group of environmental pollutants, known for their mutagenic and carcinogenic activities. Many PAHs are aryl hydrocarbon receptor (AhR) ligands and several recent studies have suggested that PAHs or their metabolites may activate estrogen receptors (ER). The pre...

    journal_title:Toxicology letters

    pub_type: 杂志文章


    authors: Kummer V,Masková J,Zralý Z,Neca J,Simecková P,Vondrácek J,Machala M

    更新日期:2008-08-28 00:00:00