Abstract：
:Although formaldehyde (FA) has been classified as a human leukemogen, the mechanisms of leukemogenesis remain elusive. Previously, using colony-forming assays in semi-solid media, we showed that FA exposure in vivo and in vitro was toxic to human hematopoietic stem/progenitor cells. In the present study, we have applied new liquid in vitro erythroid expansion systems to further investigate the toxic effects of FA (0-150 μM) on cultured mouse and human hematopoietic stem/progenitor cells. We determined micronucleus (MN) levels in polychromatic erythrocytes (PCEs) differentiated from mouse bone marrow. We measured cell growth, cell cycle distribution, and chromosomal instability, in erythroid progenitor cells (EPCs) expanded from human peripheral blood mononuclear cells. FA significantly induced MN in mouse PCEs and suppressed human EPC expansion in a dose-dependent manner, compared with untreated controls. In the expanded human EPCs, FA slightly increased the proportion of cells in G2/M at 100 μM and aneuploidy frequency in chromosomes 7 and 8 at 50 μM. Our findings provide further evidence of the toxicity of FA to hematopoietic stem/progenitor cells and support the biological plausibility of FA-induced leukemogenesis.
journal_name
Toxicol Lettjournal_title
Toxicology lettersauthors
Ji Z,Li X,Fromowitz M,Mutter-Rottmayer E,Tung J,Smith MT,Zhang Ldoi
10.1016/j.toxlet.2013.10.028subject
Has Abstractpub_date
2014-01-13 00:00:00pages
233-9issue
2eissn
0378-4274issn
1879-3169pii
S0378-4274(13)01371-4journal_volume
224pub_type
杂志文章abstract：:The effect of a single intravenous (i.v.) injection of cadmium nitrate was investigated in livers of male Wistar rats. A significant increase in liver weight, accompanied by an elevation of total hepatic DNA content was observed. DNA synthesis as measured by the incorporation of [3H]thymidine, was found to be 6 times ...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(84)90020-1
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2011.02.011
更新日期：2011-05-10 00:00:00
abstract：:In this work the in vitro reactions of phosphine with intact red blood cells and membrane-free hemoglobin extracts are reported. We demonstrate that phosphine or phosphine derivatives induce dense aggregates of denatured hemoglobin known as 'Heinz bodies' in intact red blood cells. The reaction products include irreve...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(91)90117-o
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abstract：:Pneumotoxicity and lethality resulting from administration of methylcyclopentadienyl manganese tricarbonyl (MMT) and its 2 major metabolites in rats were compared. Following i.p. injection, MMT was found to have an LD50 value of 12.1 mg/kg. Neither of the metabolites appeared to have significant acute toxicity even wh...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(87)90250-5
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journal_title：Toxicology letters
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journal_title：Toxicology letters
pub_type： 杂志文章,评审
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2003.09.003
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journal_title：Toxicology letters
pub_type： 杂志文章,评审
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journal_title：Toxicology letters
pub_type： 杂志文章,评审
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journal_title：Toxicology letters
pub_type： 杂志文章
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journal_title：Toxicology letters
pub_type： 杂志文章,评审
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更新日期：1995-05-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(81)90057-6
更新日期：1981-02-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2006.10.012
更新日期：2007-01-10 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2013.08.003
更新日期：2013-10-09 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2014.02.001
更新日期：2014-10-15 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2014.01.004
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2003.12.053
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abstract：:Dopaminergic system dysfunction is proved to be a possible mechanism in acrylamide (ACR) -induced neurotoxicity. The neurotransmitter dopamine (DA) has an increasingly important role in the dopaminergic system. Thus, the goal of this study is to evaluate effects of ACR on dopamine and its metabolite levels, dopamine t...
journal_title：Toxicology letters
pub_type： 杂志文章
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journal_title：Toxicology letters
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journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：2007-09-10 00:00:00
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journal_title：Toxicology letters
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journal_title：Toxicology letters
pub_type： 杂志文章
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journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：2018-06-01 00:00:00
abstract：:The U.S. EPA first signalled its intention to use benchmark dose risk techniques in 1991. Subsequently, publication of draft Guidelines for the Risk Assessment of Reproductive Toxicity data indicated the Agency's intention for wide use of the technique. In developmental toxicity experiments, a number of factors need t...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(95)03584-2
更新日期：1995-12-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：1995-05-01 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
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更新日期：1997-07-21 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2020.06.006
更新日期：2020-10-10 00:00:00
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journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/j.toxlet.2004.03.004
更新日期：2004-07-15 00:00:00
abstract：:By the gas plant at Schwaan (a small city near Rostock), tar and other by-products were released into the river Warnow which supplies Rostock with drinking water 20 km downstream. The tar-contaminated river sediment contained phenols and polycyclic aromatic hydrocarbons (PAH), indicating a mutagenic potential. Investi...
journal_title：Toxicology letters
pub_type： 杂志文章
doi：10.1016/0378-4274(96)03761-7
更新日期：1996-11-01 00:00:00
