Abstract：
:We tested the hypothesis that hypertension associated with polycythemia vera (PV) may be related to hemoglobin released from erythrocytes (cell-free hemoglobin, fHb). We assessed hematocrit, mean arterial pressure (MAP), blood viscosity, and the level of fHb and nitrite/nitrate (NOx) in the plasma of 73 PV patients and 38 healthy controls. The effect of isovolemic erythrocytapheresis (ECP) on the considered parameters was also studied. From the whole group of PV patients a subset of subjects with normal (normotensive patients, n = 16) and elevated MAP (hypertensive patients, n = 57) can be subtracted. It was found that in comparison with healthy controls, PV patients have significantly (p ≤ 0.01) elevated Hct (0.567 vs. 0.422), blood viscosity (5.45 vs. 3.56 cP), MAP (106.8 vs. 93.8 mmHg), plasma fHb (9.7 vs. 2.8 mg/dL), and NOx levels (34.1 vs. 27.5 μM). Compared with normotensive patients, hypertensive PV patients demonstrated a higher rise in fHb (10.2 vs. 8.0) and plasma NOx levels (35.8 vs. 31.0). In PV patients, fHb positively correlates with MAP (r = 0.489), NOx levels (r = 0.461), hematocrit (r = 0.428), and viscosity (r = 0.393). Blood viscosity positively correlated with hematocrit (r = 0.894), but not with other considered parameters. In PV patients MAP poorly correlated with hematocrit, whereas the correlation between MAP and NOx altered from - 0.325 (healthy control) to + 0.268 (PV patients). ECP procedure was associated with a significant (p < 0.01) reduction of hematocrit, fHb, blood viscosity, and MAP. In the normotensive subgroup of PV patients the ECP procedure did not affect MAP. It can be concluded that accelerated scavenging of nitric oxide by fHb rather than high Hct may be a key factor determining the development of hypertension in PV patients.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Rusak T,Misztal T,Piszcz J,Tomasiak Mdoi
10.3109/10715762.2013.860225subject
Has Abstractpub_date
2014-02-01 00:00:00pages
230-8issue
2eissn
1071-5762issn
1029-2470journal_volume
48pub_type
杂志文章abstract：:Novel silylated triarylmethyl (TAM) radicals based on TAM core CT-03 and their electron paramagnetic resonance (EPR) spectra are evaluated as a function of oxygen concentration. Combination of peak-to-peak linewidth of the EPR signal and electrochemical determination allows designing a method for oxygen quantification...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715762.2014.995183
更新日期：2015-03-01 00:00:00
abstract：:Effects of 12 wk exercise training on oxidative stress were examined in elderly humans. We measured oxidative stress during a 45 min cycling test by using antipyrine hydroxylation products. Antipyrine breakdown is independent of blood flow to the liver, which is important during exercise. Furthermore, antipyrine react...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760100300951
更新日期：2001-10-01 00:00:00
abstract：:We investigated hepatoprotective activity and antioxidant effect of the 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-trans-stilbene that purified from Morus bombycis Koidzumi roots against CCl4-induced liver damage in rats. The 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-trans-stilbene displayed dose-dependent ...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760600803823
更新日期：2006-09-01 00:00:00
abstract：:In severe COPD patients, oxidative stress, which is involved in their peripheral muscle dysfunction, increases in response to exercise. In this study, muscle oxidative stress was explored after quadriceps magnetic stimulation training. A randomized controlled study was conducted on very severe COPD patients, who under...
journal_title：Free radical research
pub_type： 杂志文章,随机对照试验
doi：10.1080/10715760802555569
更新日期：2008-11-01 00:00:00
abstract：:The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up in 1997 to resolve methodological problems and to reach agreement on the basal level of 8-oxo-2'-deoxyguanosine (8-oxodG) in biological samples. In the present ESCODD trial 6 samples of 8-oxodG-containing oligonucleotides with different ratio...
journal_title：Free radical research
pub_type： 杂志文章,多中心研究
doi：10.1080/10715760290029047
更新日期：2002-06-01 00:00:00
abstract：:Nerve growth factor (NGF) is one of the several structurally related proteins, named neurotrophins (NTs), that regulate neuronal survival, development, function, and plasticity. Moreover, NGF is an important activator of antioxidant mechanisms. These NGF functions are mediated by tropomyosin-related kinase receptor A ...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715762.2013.783210
更新日期：2013-05-01 00:00:00
abstract：:Gastric inflammation is a highly complex biochemical protective response to cellular/tissue injury. When this process occurs in an uncontrolled manner, the result is excessive cellular/tissue damage that results chronic inflammation and destruction of normal tissue. Current evidence suggests that Helicobacter pylori (...
journal_title：Free radical research
pub_type： 杂志文章,评审
doi：10.1080/10715760000301301
更新日期：2000-12-01 00:00:00
abstract：:Parkinson's disease (PD) is a neurodegenerative disorder characterised by selective degeneration of the nigral dopaminergic neurons, and neuroinflammation and oxidative stress are believed to be involved in its pathogenesis. In the present study, we provide data that the synthetic steroid exemestane, which is currentl...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715762.2017.1353688
更新日期：2017-06-01 00:00:00
abstract：:We studied the effects of a single post whole-body low-dose irradiation (50 cGy of gamma-ray) on mice with ferric nitrilotriacetate (Fe3+-NTA)-induced transient hepatopathy. As a result, low-dose irradiation accelerated the rate of recovery. Based on the changes in glutamic oxaloacetic transaminase (GOT) activities, g...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760000300221
更新日期：2000-03-01 00:00:00
abstract：OBJECTIVES:The aim of the present study was to investigate the potential importance of oxidative stress, measured by isoprostanes-related compounds, as non-traditional risk factor for cardiovascular disease. We planned to examine the relationship between concentrations of plasma F₂-isoprostanes (F₂-IsoPs), isofurans (I...
journal_title：Free radical research
pub_type： 杂志文章,随机对照试验
doi：10.3109/10715762.2012.702900
更新日期：2012-10-01 00:00:00
abstract：:The aim of this study was to test the hypothesis that pulmonary inflammation and emphysema induced by cadmium (Cd) inhalation are associated with pulmonary oxidative stress. Two groups of Sprague Dawley rats were used: one vehicle-exposed group undergoing inhalation of NaCl (0.9%, n = 24) and one Cd-exposed group unde...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760500494657
更新日期：2006-03-01 00:00:00
abstract：:Soy sauce is a traditional fermented seasoning in Asian countries, that has high antioxidant activity in vitro and some antioxidant activity in vivo. We attempted to identify the major antioxidants present, using the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay as a guide. 3-Hydroxy-2-methyl-4H-p...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760601110871
更新日期：2007-04-01 00:00:00
abstract：:To solve the problems faced in clinical use of hypocrellins, a water-soluble preparation of Hypocrellin B (HB), HB-Triton X-100 (TX-100) micelles, was prepared. To evaluate the photodynamic activity, the free radicals (*OH and HB*-) and singlet oxygen (1O2) generated via photosensitization of the preparation in aqueou...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760310001601056
更新日期：2003-10-01 00:00:00
abstract：:Silibinin is an active constituent extracted from blessed milk thistle (Silybum marianum). Our previous study demonstrated that silibinin induced autophagy and apoptosis via reactive oxygen species (ROS) generation in HeLa cells. In this study, we investigated whether the autophagy- and apoptosis-associated molecules ...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715762.2012.655244
更新日期：2012-03-01 00:00:00
abstract：:Microcystin-LR (MCLR) is a potent hepatotoxin. Oxidative stress is thought to be implicated in the cytotoxicity of MCLR, but the mechanisms by which MCLR produces reactive oxygen species (ROS) are still unclear. This study investigated the role and possible sources of ROS generation in MCLR-induced cytogenotoxicity in...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760701704599
更新日期：2007-12-01 00:00:00
abstract：:Anxiety disorders are the most common mental illness in the USA affecting 18% of the population. The cause(s) of anxiety disorders is/are not completely clear, and research in the neurobiology of anxiety at the molecular level is still rather limited. Although mounting clinical and preclinical evidence now indicates t...
journal_title：Free radical research
pub_type： 杂志文章,评审
doi：10.1080/10715762.2018.1475733
更新日期：2018-07-01 00:00:00
abstract：:The classical functions of p53 protein are those related to its role on DNA damage, cell growth arrest, senescence and apoptosis. For this reason it is called 'the guardian of the genome' and is considered one of the most important players in the development of cancer. However, more recently it has been show that p53 ...
journal_title：Free radical research
pub_type： 杂志文章,评审
doi：10.3109/10715762.2011.571685
更新日期：2011-06-01 00:00:00
abstract：:There is a correlation between oxidative stress generated by diethylnitrosamine (DEN) metabolism and liver cancer development. Quercetin is a flavonoid with anti-carcinogenic and antioxidant properties. This study demonstrates the mechanism of action for the chemopreventive effect of quercetin. A 10 mg/kg dose of quer...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760802626535
更新日期：2009-02-01 00:00:00
abstract：:Recovery of neurological function in patients following cardiac arrest and cardiopulmonary resuscitation (CPR) is a complex event. Free radical induced oxidative stress is supposed to be involved in this process. We studied levels of 8-iso-PGF2alpha (indicating oxidative injury) and 15-keto-dihydro-PGF2alpha (indicati...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/1071576021000043058
更新日期：2003-03-01 00:00:00
abstract：:An iodoacetamide azaadamantyl spin label was studied in fluid solution and in 9:1 trehalose:sucrose glass. In 9:1 toluene:CH2Cl2 solution at 293 K, the isotropic nitrogen hyperfine coupling is 19.2 G, T1 is 0.37 µs and T2 is 0.30-0.35 µs. Between about 80 and 150 K 1/Tm in 9:1 trehalose:sucrose is approximately indepe...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715762.2017.1384954
更新日期：2018-03-01 00:00:00
abstract：:Septic shock, a severe form of sepsis, is characterized by cardiovascular collapse following microbial invasion of the body. The progressive hypotension, hyporeactivity to vasopressor agents and vascular leak leads to circulatory failure with multiple organ dysfunction and death. Many inflammatory mediators (e.g. TNF-...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760410001725517
更新日期：2004-11-01 00:00:00
abstract：:Epidemiologically, a high-carotenoid intake via a fruit- and vegetable-rich diet is associated with a decreased risk of various forms of cancer. The mechanisms by which carotenoids exert this protective effect are controversial. In this study, we examined the potency of a range of carotenoids commonly found in human p...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760290032539
更新日期：2002-07-01 00:00:00
abstract：:Silver nanoparticles (AgNPs) are the most widely used nanomaterials as antimicrobial agents. AgNPs have been shown to inhibit the growth of and induce apoptosis-like death in Escherichia coli. However, the precise mechanism of AgNPs-induced apoptosis-like death and association with DNA damage-inducible protein F (dinF...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715762.2020.1866178
更新日期：2021-01-06 00:00:00
abstract：:We describe an altered membrane band 3 protein-mediated anion transport in erythrocytes exposed to peroxynitrite, and relate the loss of anion transport to cell damage and to band 3 oxidative modifications. We found that peroxynitrite down-regulate anion transport in a dose dependent relation (100-300 micromoles/l). H...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760601090305
更新日期：2007-03-01 00:00:00
abstract：:The present study was designed to determine whether hydroxymethylglutaryl-CoA reductase inhibitors (statins) modulate the NO production via iNOS in adipocytes stimulated by lipopolysaccharide (L) and tumour necrosis factor-alpha (T). Well-differentiated 3T3-L1 adipocytes significantly produced NO by LT-treatment. Pre-...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760701534368
更新日期：2007-09-01 00:00:00
abstract：:Oxidation of low density lipoprotein (LDL) has been implicated in the pathogenesis of atherosclerosis. The most common technique for measuring the oxidation of lipoproteins is the continuous measurement of the formation of conjugated diene at OD 234 nm. The concept of "lag time", derived from such measurements, has be...
journal_title：Free radical research
pub_type： 杂志文章,评审
doi：10.3109/10715769809065814
更新日期：1998-06-01 00:00:00
abstract：BACKGROUND:Measurement of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) has recently become more popular as a means of assessing oxidative stress in the human body. The aim of this study is to compare the levels of urine 8-OHdG in patients with type 2 diabetes with and without nephropathy and to evaluate its role as a b...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715762.2012.710902
更新日期：2012-10-01 00:00:00
abstract：:The phenomenon of ozone tolerance is described, but the underlying mechanisms remain unknown. We tested whether adaptation to multiday ozone exposure was related to an upregulated pulmonary antioxidant defence. Six calves were exposed to 0.75 ppm ozone, 12 h day(-1) for seven consecutive days. Pulmonary function tests...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715760210169
更新日期：2002-01-01 00:00:00
abstract：:The objective of this study was to elucidate the impact of physical activity during the growth period as well as on oxidative stress and antioxidative potential in adulthood. The experimental animals used were four-week old male Wistar rats, which were randomly divided into three groups. The exercise loads were as fol...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.1080/10715762.2017.1291939
更新日期：2017-02-01 00:00:00
abstract：:Orellanine is the tetrahydroxylated and di-N-oxidized bipyridine toxin extracted from several Cortinarius mushrooms among them C. orellanus. The pathogenic mechanism involved in the C. orellanus-poisoning by orellanine leading to kidney impairment is not yet fully understood until now. Electron spin resonance (ESR) sp...
journal_title：Free radical research
pub_type： 杂志文章
doi：10.3109/10715769809066887
更新日期：1998-05-01 00:00:00
