Abstract:
:Major histocompatibility complex (MHC) class II molecule-expressing cells and macrophages play a pivotal role in mediating the host tissue response to biomaterials. This study investigated the responses of these cells to epoxy resin-based and 4-META-containing, methacrylate resin-based endodontic sealers (AH Plus and MetaSEAL respectively) in rat connective tissue. Silicone tubes loaded with one of the sealers or solid silicone rods (control) were subcutaneously implanted in male Wistar rats for three time periods of 7, 14, or 28 days. Tissue specimens were immunoperoxidase-stained for MHC class II molecules and CD68 (a general macrophage marker). Results showed that AH Plus-implanted tissue displayed significantly more MHC class II-positive cells than the control at 14 and 28 days, whereas MetaSEAL-implanted tissue showed significantly more CD68-positive cells than both AH Plus-implanted tissue and the control at all time periods. It was concluded that the epoxy resin-based sealer induced the infiltration of MHC class II molecule-expressing cells, whereas 4-META-containing, methacrylate resin-based sealer elicited macrophage infiltration.
journal_name
Dent Mater Jjournal_title
Dental materials journalauthors
Yamanaka Y,Shigetani Y,Yoshiba K,Kaneko T,Yoshiba N,Okiji Tdoi
10.4012/dmj.2013-005subject
Has Abstractpub_date
2013-01-01 00:00:00pages
822-7issue
5eissn
0287-4547issn
1881-1361pii
DN/JST.JSTAGE/dmj/2013-005journal_volume
32pub_type
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