Raman hyperspectral imaging of microfossils: potential pitfalls.

Abstract:

:Initially, Raman spectroscopy was a specialized technique used by vibrational spectroscopists; however, due to rapid advancements in instrumentation and imaging techniques over the last few decades, Raman spectrometers are widely available at many institutions, allowing Raman spectroscopy to become a widespread analytical tool in mineralogy and other geological sciences. Hyperspectral imaging, in particular, has become popular due to the fact that Raman spectroscopy can quickly delineate crystallographic and compositional differences in 2-D and 3-D at the micron scale. Although this rapid growth of applications to the Earth sciences has provided great insight across the geological sciences, the ease of application as the instruments become increasingly automated combined with nonspecialists using this techique has resulted in the propagation of errors and misunderstandings throughout the field. For example, the literature now includes misassigned vibration modes, inappropriate spectral processing techniques, confocal depth of laser penetration incorrectly estimated into opaque crystalline solids, and a misconstrued understanding of the anisotropic nature of sp² carbons.

journal_name

Astrobiology

journal_title

Astrobiology

authors

Marshall CP,Olcott Marshall A

doi

10.1089/ast.2013.1034

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

920-31

issue

10

eissn

1531-1074

issn

1557-8070

journal_volume

13

pub_type

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