Abstract:
:Plasmids were constructed in which a HindIII fragment of rDNA (6.4 x 10(3) base-pairs) was inserted into vectors pGEM-1 and 2 in both orientations. The DNA insert encoded the yeast 35 S precursor rRNA beginning 180 bases upstream from the 5' end of the mature 18 S rRNA and ending 289 bases beyond the 3' end of the mature 25 S rRNA. The precursor rRNA molecules produced in vitro consisted of 6430 nucleotides, with about 15 residues derived from the Gemini vector on both ends. The general extent of secondary structure of the precursor rRNA was examined by ethidium fluorescence and compared to that of the mature rRNAs. Both precursor and mature rRNAs responded similarly to changes in magnesium ion concentration and to digestion by cobra venom and T1 ribonucleases. The higher-order structure of the internal transcribed spacer-1 (ITS-1) region of the 35 S rRNA molecule was further examined by kethoxal and dimethylsulfate modifications and primer extension. Accessible adenine and guanine residues were located by primer extension analysis with avian myeloblastosis virus reverse transcriptase. On the basis of experimental data and computer-generated structures, a secondary structure model was proposed for the ITS-1 region. In this model, six hairpin stems involving adjacent nucleotides are present. A long-range interaction between nucleotides at the middle of the ITS-1 region and an, as yet, unidentified sequence located at another region of the precursor rRNA is suggested also. A candidate for this interacting sequence is that previously proposed, on a theoretical basis, to be involved in the removal of the precursor 18 S rRNA species for 35 S precursor molecule.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Thweatt R,Lee JCdoi
10.1016/0022-2836(90)90353-Nsubject
Has Abstractpub_date
1990-01-20 00:00:00pages
305-20issue
2eissn
0022-2836issn
1089-8638pii
0022-2836(90)90353-Njournal_volume
211pub_type
杂志文章abstract::Aminoacyl-tRNA synthetases are validated molecular targets for anti-infective drug discovery because of their essentiality in protein synthesis. Thanks to genome sequencing, it is now possible to systematically study aminoacyl-tRNA synthetases from human eukaryotic parasites as putative targets for novel drug discover...
journal_title:Journal of molecular biology
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abstract::RGG/RG motifs are RNA binding segments found in many proteins that can partition into membraneless organelles. They occur in the context of low-complexity disordered regions and often in multiple copies. Although short RGG/RG-containing regions can sometimes form high-affinity interactions with RNA structures, multipl...
journal_title:Journal of molecular biology
pub_type: 杂志文章,评审
doi:10.1016/j.jmb.2018.06.014
更新日期:2018-11-02 00:00:00
abstract::The work reported here describes insertion-deletion (Indel) polymorphisms in two internally eliminated sequences (IESs, that are deleted during development in Tetrahymena): a 1.8 kb Indel at one end of the 1.1 kb H1 IES and a 0.5 kb Indel inside the 1.4 kb calmodulin (C) IES. These two IESs are located in the proximit...
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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更新日期:1991-10-05 00:00:00
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journal_title:Journal of molecular biology
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pub_type: 杂志文章
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更新日期:1990-01-05 00:00:00
abstract::Aminoacylation and editing by leucyl-tRNA synthetases (LeuRS) require migration of the tRNA acceptor stem end between the canonical aminoacylation core and a separate domain called CP1 that is responsible for amino acid editing. The LeuRS CP1 domain can also support group I intron RNA splicing in the yeast mitochondri...
journal_title:Journal of molecular biology
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doi:10.1016/j.jmb.2006.12.051
更新日期:2007-03-23 00:00:00
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journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
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abstract::A complex pseudoknot structure surrounds the first ribosome initiation site in the Escherichia coli alpha mRNA and mediates its regulation by ribosomal protein S4. A 112 nt RNA fragment containing this pseudoknot exists in two conformations that are resolvable by gel electrophoresis below room temperature. Between 30 ...
journal_title:Journal of molecular biology
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journal_title:Journal of molecular biology
pub_type: 杂志文章,评审
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journal_title:Journal of molecular biology
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doi:10.1016/0022-2836(88)90582-7
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journal_title:Journal of molecular biology
pub_type: 杂志文章
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journal_title:Journal of molecular biology
pub_type: 杂志文章,评审
doi:10.1016/j.jmb.2006.09.059
更新日期:2007-01-05 00:00:00
abstract::Arg15 is a conserved active-site residue in class Alpha glutathione transferases. X-ray diffraction studies of human glutathione transferase A1-1 have shown that N epsilon of this amino acid residue is adjacent to the sulfur atom of a glutathione derivative bound to the active site, suggesting the presence of a hydrog...
journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/s0022-2836(05)80154-8
更新日期:1995-04-07 00:00:00
abstract::Isoniazid (INH) was the first synthesized drug that mediated bactericidal killing of the bacterium Mycobacterium tuberculosis, a major clinical breakthrough. To this day, INH remains a cornerstone of modern tuberculosis (TB) chemotherapy. This review describes the serendipitous discovery of INH, its effectiveness on T...
journal_title:Journal of molecular biology
pub_type: 杂志文章,评审
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更新日期:2019-08-23 00:00:00
abstract::The motion of the atoms in the small protein bovine pancreatic trypsin inhibitor has been simulated for about 60 picoseconds using two different potential energy functions. In one known as HHL the hydrogen bond is purely electrostatic, in the other, known as L79, the hydrogen bond is a directional O . . . H interactio...
journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1016/s0022-2836(83)80304-0
更新日期:1983-08-15 00:00:00
abstract::We succeeded in expressing a chimeric myosin that comprises the motor domain of characean algal myosin, (the fastest known motor protein), and the neck and tail domains of Dictyostelium myosin II. Although the chimeric myosin showed an ATPase activity comparable to that of muscle myosin (15 times higher than that of t...
journal_title:Journal of molecular biology
pub_type: 杂志文章
doi:10.1006/jmbi.2001.4883
更新日期:2001-08-17 00:00:00
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journal_title:Journal of molecular biology
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doi:10.1016/j.jmb.2009.01.043
更新日期:2009-03-27 00:00:00