Abstract:
:The respiratory complex I (electrogenic NADH:quinone oxidoreductase) has been considered to act exclusively as a H+ pump. This was questioned when the search for the NADH-driven respiratory Na+ pump in Klebsiella pneumoniae initiated by Peter Dimroth led to the discovery of a Na+-translocating complex in this enterobacterium. The 3D structures of complex I from different organisms support the idea that the mechanism of cation transport by complex I involves conformational changes of the membrane-bound NuoL, NuoM and NuoN subunits. In vitro methods to follow Na+ transport were compared with in vivo approaches to test whether complex I, or its individual NuoL, NuoM or NuoN subunits, extrude Na+ from the cytoplasm to the periplasm of bacterial host cells. The truncated NuoL subunit of the Escherichia coli complex I which comprises amino acids 1-369 exhibits Na+ transport activity in vitro. This observation, together with an analysis of putative cation channels in NuoL, suggests that there exists in NuoL at least one continuous pathway for cations lined by amino acid residues from transmembrane segments 3, 4, 5, 7 and 8. Finally, we discuss recent studies on Na+ transport by mitochondrial complex I with respect to its putative role in the cycling of Na+ ions across the inner mitochondrial membrane.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Steffen W,Steuber Jdoi
10.1042/BST20130024subject
Has Abstractpub_date
2013-10-01 00:00:00pages
1280-7issue
5eissn
0300-5127issn
1470-8752pii
BST20130024journal_volume
41pub_type
杂志文章,评审abstract::Mutations in the LMNA gene, encoding the nuclear intermediate filaments the A-type lamins, result in a wide variety of diseases known as laminopathies. Some of them, such as familial partial lipodystrophy of Dunnigan and metabolic laminopathies, are characterized by lipodystrophic syndromes with altered fat distributi...
journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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更新日期:2013-02-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20190312
更新日期:2020-04-29 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0380934
更新日期:2010-08-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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更新日期:2005-08-01 00:00:00
abstract::AMP-activated protein kinase (AMPK) is a regulator of cellular metabolism in response to changes in the energy status of the cells. AMPK was known to shut down energy-consuming pathways in response to a fall in the ATP/AMP ratio by phosphorylating key enzymes of intermediate metabolism. Here we will discuss the recent...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/
更新日期:2002-04-01 00:00:00
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journal_title:Biochemical Society transactions
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doi:10.1042/BST20150236
更新日期:2016-04-15 00:00:00
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journal_title:Biochemical Society transactions
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更新日期:2012-02-01 00:00:00
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journal_title:Biochemical Society transactions
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更新日期:2019-12-20 00:00:00
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journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
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更新日期:1984-12-01 00:00:00
abstract::The Kelch-like ECH associated protein 1 (Keap1)-NF-E2 p45-related factor 2 (Nrf2) pathway regulates networks of proteins that protect against the cumulative damage of oxidants, electrophiles and misfolded proteins. The interaction between transcription factor Nrf2 and its main negative cytoplasmic regulator Keap1 foll...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
abstract::One of the primary functions of AMP-activated protein kinase (AMPK) is to regulate the metabolic pathways in response to reduced cellular energy charge. Most of the known targets of the kinase are cytoplasmic enzymes involved in both catabolic and anabolic metabolism. In addition, activation of AMPK in many cells resu...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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更新日期:2003-02-01 00:00:00
abstract::Despite decades of research, many aspects of the biology of Mycobacterium tuberculosis remain unclear, and this is reflected in the antiquated tools available to treat and prevent tuberculosis and consequently this disease remains a serious public health problem. Important discoveries linking the metabolism of M. tube...
journal_title:Biochemical Society transactions
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更新日期:2010-10-01 00:00:00
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journal_title:Biochemical Society transactions
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更新日期:2004-04-01 00:00:00
abstract::Whereas we have a profound understanding about the function and biogenesis of the protein constituents in the lumen of the lysosomal compartment, much less is known about the functions of proteins of the lysosomal membrane. Proteomic analyses of the lysosomal membrane suggest that, apart from the well-known lysosomal ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
abstract::Insect and mammalian circadian clocks show striking similarities. They utilize homologous clock genes, generating self-sustained circadian oscillations in distinct master clocks of the brain, which then control rhythmic behaviour. The molecular mechanisms of rhythm generation were first uncovered in the fruit fly Dros...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
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更新日期:2005-11-01 00:00:00