Raman spectroscopy as a non-invasive technique for the quantification of melanins in feathers and hairs.

Abstract:

:The quantification of melanins is a complex task due to the chemical heterogeneity of the pigments and the difficulty of their isolation. The best accepted procedure currently consists in the chemical cleavage of melanins and the subsequent detection of degradation products by HPLC, which implies the destruction of samples. Here, we show that Raman spectroscopy is a non-invasive technique that can be used to quantify melanins. We made parallel analyses of the characteristics of pheomelanin and eumelanin Raman spectra as measured by confocal Raman microscopy and of degradation products of pheomelanin (4-amino-3-hydroxyphenylalanine, 4-AHP) and eumelanin (pyrrole-2,3,5-tricarboxylic acid, PTCA) as measured by HPLC in feathers of red-legged partridges and hairs of wild boars and humans. We found strong correlations between the spectral Raman characteristics and 4-AHP and PTCA levels, which indicates that the Raman spectra of melanins can be used to determine their content.

authors

Galván I,Jorge A,Ito K,Tabuchi K,Solano F,Wakamatsu K

doi

10.1111/pcmr.12140

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

917-23

issue

6

eissn

1755-1471

issn

1755-148X

journal_volume

26

pub_type

杂志文章
  • Genetic variation in regulatory DNA elements: the case of OCA2 transcriptional regulation.

    abstract::Mutations within the OCA2 gene or the complete absence of the OCA2 protein leads to oculocutaneous albinism type 2. The OCA2 protein plays a central role in melanosome biogenesis, and it is a strong determinant of the eumelanin content in melanocytes. Transcript levels of the OCA2 gene are strongly correlated with pig...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章,评审

    doi:10.1111/pcmr.12210

    authors: Visser M,Kayser M,Grosveld F,Palstra RJ

    更新日期:2014-03-01 00:00:00

  • The tumor suppressor BAP1 cooperates with BRAFV600E to promote tumor formation in cutaneous melanoma.

    abstract::The deubiquitinating enzyme BAP1 is mutated in a hereditary cancer syndrome with a high risk of mesothelioma and melanocytic tumors. Here, we show that Bap1 deletion in melanocytes cooperates with the constitutively active, oncogenic form of BRAF (BRAFV600E ) and UV to cause melanoma in mice, albeit at very low freque...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12735

    authors: Webster JD,Pham TH,Wu X,Hughes NW,Li Z,Totpal K,Lee HJ,Calses PC,Chaurushiya MS,Stawiski EW,Modrusan Z,Chang MT,Tran C,Lee WP,Chalasani S,Hung J,Sharma N,Chan S,Hotzel K,Talevich E,Shain A,Xu M,Lill J,Dixit

    更新日期:2019-03-01 00:00:00

  • PLX3397 inhibits the accumulation of intra-tumoral macrophages and improves bromodomain and extra-terminal inhibitor efficacy in melanoma.

    abstract::Bromodomain and extra-terminal inhibitors (BETi) delay tumor growth, in part, through tumor cell intrinsic alterations and initiation of anti-tumor CD8+ T-cell responses. By contrast, BETi effects on pro-tumoral immune responses remain unclear. Here, we show that the next-generation BETi, PLX51107, delayed tumor growt...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12845

    authors: Erkes DA,Rosenbaum SR,Field CO,Chervoneva I,Villanueva J,Aplin AE

    更新日期:2020-03-01 00:00:00

  • Identification of PLX4032-resistance mechanisms and implications for novel RAF inhibitors.

    abstract::BRAF inhibitors improve melanoma patient survival, but resistance invariably develops. Here we report the discovery of a novel BRAF mutation that confers resistance to PLX4032 employing whole-exome sequencing of drug-resistant BRAF(V600K) melanoma cells. We further describe a new screening approach, a genome-wide pigg...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12197

    authors: Choi J,Landrette SF,Wang T,Evans P,Bacchiocchi A,Bjornson R,Cheng E,Stiegler AL,Gathiaka S,Acevedo O,Boggon TJ,Krauthammer M,Halaban R,Xu T

    更新日期:2014-03-01 00:00:00

  • Vitiligo as an inflammatory skin disorder: a therapeutic perspective.

    abstract::From a therapeutic standpoint, vitiligo is still regarded by many physicians as a simple problem of regenerative medicine, with the main aim to repopulate the depigmented skin with functional melanocytes from the margins of the lesions or from intact progenitors in hair follicles. However, recent research in vitiligo ...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章,评审

    doi:10.1111/j.1755-148X.2011.00939.x

    authors: Taïeb A

    更新日期:2012-01-01 00:00:00

  • Genomic characterisation of acral melanoma cell lines.

    abstract::Acral melanoma is a rare melanoma subtype with distinct epidemiological, clinical and genetic features. To determine if acral melanoma cell lines are representative of this melanoma subtype, six lines were analysed by whole-exome sequencing and array comparative genomic hybridisation. We demonstrate that the cell line...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/j.1755-148X.2012.01016.x

    authors: Furney SJ,Turajlic S,Fenwick K,Lambros MB,MacKay A,Ricken G,Mitsopoulos C,Kozarewa I,Hakas J,Zvelebil M,Lord CJ,Ashworth A,Reis-Filho JS,Herlyn M,Murata H,Marais R

    更新日期:2012-07-01 00:00:00

  • Silencing and re-expression of retinoic acid receptor beta2 in human melanoma.

    abstract::Many melanoma cells are resistant to the anti-proliferative effect of all trans retinoic acid (ATRA). Retinoic Acid Receptor-beta2 (RAR-beta2) mediates the ATRA growth inhibition. We found a correlation between the anti-proliferative activity of ATRA and expression of RAR-beta2. There was not a strict correlation betw...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/j.1755-148X.2010.00702.x

    authors: Fan J,Eastham L,Varney ME,Hall A,Adkins NL,Chetel L,Sollars VE,Georgel P,Niles RM

    更新日期:2010-06-01 00:00:00

  • Postnatal lineage mapping of follicular melanocytes with the Tyr::CreER(T) (2) transgene.

    abstract::One of the main advantages of using inducible and conditional transgenes to study pigment cell biology is that they allow for genetic manipulation within melanocytes after roles in general neural crest or melanoblast development have been fulfilled. Specifically, we focus here on the ability of the Tyr::CreER(T) ...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12048

    authors: Harris ML,Pavan WJ

    更新日期:2013-03-01 00:00:00

  • Independent regulation of hair and skin color by two G protein-coupled pathways.

    abstract::Hair color and skin color are frequently coordinated in mammalian species. To explore this, we have studied mutations in two different G protein coupled pathways, each of which affects the darkness of both hair and skin color. In each mouse mutant (Gnaq(Dsk1), Gna11(Dsk7), and Mc1r(e)), we analyzed the melanocyte dens...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/j.1755-148X.2009.00609.x

    authors: Van Raamsdonk CD,Barsh GS,Wakamatsu K,Ito S

    更新日期:2009-12-01 00:00:00

  • T-type calcium channel blockers inhibit autophagy and promote apoptosis of malignant melanoma cells.

    abstract::We have recently reported that human melanoma cells express a variety of voltage-gated calcium (Ca(2+) ) channel types, including low-voltage-activated T-type channels that play a significant role in melanoma cell cycle progression. Here, we challenged melanoma metastatic cells with T-type channel blockers of clinical...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12155

    authors: Das A,Pushparaj C,Herreros J,Nager M,Vilella R,Portero M,Pamplona R,Matias-Guiu X,Martí RM,Cantí C

    更新日期:2013-11-01 00:00:00

  • Lighting a path to pigmentation: mechanisms of MITF induction by UV.

    abstract::While sunlight is important for life, ultraviolet radiation (UVR) can have harmful and mutagenic effects. This duality is particularly relevant to human skin, in which UVR both participates in evolutionarily important photochemical reactions yet may act as a potential carcinogen. UVR can upregulate production of melan...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章,评审

    doi:10.1111/j.1755-148X.2010.00775.x

    authors: Liu JJ,Fisher DE

    更新日期:2010-12-01 00:00:00

  • Keratinocytes in culture accumulate phagocytosed melanosomes in the perinuclear area.

    abstract::There are many techniques for evaluating melanosome transfer to keratinocytes but the spectrophotometric quantification of melanosomes incorporated by keratinocyte phagocytosis has not been previously reported. Here we describe a new method that allows the spectrophotometric visualization of melanosome uptake by norma...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/j.1755-148X.2009.00640.x

    authors: Ando H,Niki Y,Yoshida M,Ito M,Akiyama K,Kim JH,Yoon TJ,Lee JH,Matsui MS,Ichihashi M

    更新日期:2010-02-01 00:00:00

  • LIGR, a protease-activated receptor-2-derived peptide, enhances skin pigmentation without inducing inflammatory processes.

    abstract::The protease-activated receptor-2 (PAR-2) is a seven transmembrane G-protein-coupled receptor that could be activated by serine protease cleavage or by synthetic peptide agonists. We showed earlier that activation of PAR-2 with Ser-Leu-Ile-Gly-Arg-Leu-NH(2) (SLIGRL), a known PAR-2 activating peptide, induces keratinoc...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/j.1755-148X.2008.00441.x

    authors: Lin CB,Chen N,Scarpa R,Guan F,Babiarz-Magee L,Liebel F,Li WH,Kizoulis M,Shapiro S,Seiberg M

    更新日期:2008-04-01 00:00:00

  • UV-induced somatic mutations elicit a functional T cell response in the YUMMER1.7 mouse melanoma model.

    abstract::Human melanomas exhibit relatively high somatic mutation burden compared to other malignancies. These somatic mutations may produce neoantigens that are recognized by the immune system, leading to an antitumor response. By irradiating a parental mouse melanoma cell line carrying three driver mutations with UVB and exp...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12591

    authors: Wang J,Perry CJ,Meeth K,Thakral D,Damsky W,Micevic G,Kaech S,Blenman K,Bosenberg M

    更新日期:2017-07-01 00:00:00

  • Pathogenesis of a variant in the 5' untranslated region of ADAR1 in dyschromatosis symmetrica hereditaria.

    abstract::Dyschromatosis symmetrica hereditaria (DSH) is a pigmentary genodermatosis caused by mutations in ADAR1. In this study, we performed mutation analysis on a family that included typical DSH patients. No mutations were found in any coding regions or exon-intron boundary regions of ADAR1, but a previously unreported non-...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12863

    authors: Suganuma M,Kono M,Yamanaka M,Akiyama M

    更新日期:2020-07-01 00:00:00

  • Distinct microRNA expression signatures are associated with melanoma subtypes and are regulated by HIF1A.

    abstract::The complex genetic changes underlying metastatic melanoma need to be deciphered to develop new and effective therapeutics. Previously, genome-wide microarray analyses of human melanoma identified two reciprocal gene expression programs, including transcripts regulated by either transforming growth factor, beta 1 (TGF...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12255

    authors: Hwang HW,Baxter LL,Loftus SK,Cronin JC,Trivedi NS,Borate B,Pavan WJ

    更新日期:2014-09-01 00:00:00

  • Melanocortin-1 receptor-mediated signalling pathways activated by NDP-MSH and HBD3 ligands.

    abstract::Binding of melanocortin peptide agonists to the melanocortin-1 receptor of melanocytes results in eumelanin production, whereas binding of the agouti signalling protein inverse agonist results in pheomelanin synthesis. Recently, a novel melanocortin-1 receptor ligand was reported. A β-defensin gene mutation was found ...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/j.1755-148X.2012.00990.x

    authors: Beaumont KA,Smit DJ,Liu YY,Chai E,Patel MP,Millhauser GL,Smith JJ,Alewood PF,Sturm RA

    更新日期:2012-05-01 00:00:00

  • PMEL: a pigment cell-specific model for functional amyloid formation.

    abstract::PMEL is a pigment cell-specific protein responsible for the formation of fibrillar sheets within the pigment organelle, the melanosome. The fibrillar sheets serve as a template upon which melanins polymerize as they are synthesized. The PMEL fibrils are required for optimal pigment cell function, as animals that eithe...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章,评审

    doi:10.1111/pcmr.12067

    authors: Watt B,van Niel G,Raposo G,Marks MS

    更新日期:2013-05-01 00:00:00

  • Genetics of human iris colour and patterns.

    abstract::The presence of melanin pigment within the iris is responsible for the visual impression of human eye colouration with complex patterns also evident in this tissue, including Fuchs' crypts, nevi, Wolfflin nodules and contraction furrows. The genetic basis underlying the determination and inheritance of these traits ha...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章,评审

    doi:10.1111/j.1755-148X.2009.00606.x

    authors: Sturm RA,Larsson M

    更新日期:2009-10-01 00:00:00

  • Integrins: versatile receptors controlling melanocyte adhesion, migration and proliferation.

    abstract::From the onset of melanocyte specification from the neural crest, throughout their migration during embryogenesis and until they reside in their niche in the basal keratinocyte layer, melanocytes interact in dynamic ways with the extracellular environment of the growing embryo. To recognize and to adhere to their envi...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章,评审

    doi:10.1111/j.1755-148X.2010.00806.x

    authors: Pinon P,Wehrle-Haller B

    更新日期:2011-04-01 00:00:00

  • Premature senescence in human melanocytes after exposure to solar UVR: An exosome and UV-miRNA connection.

    abstract::Ultraviolet radiation (UVR) can play two roles: induce cellular senescence and convert skin melanocytes into melanoma. To assess whether this conversion might rely on melanocytes having to first acquire a senescent phenotype, we studied the effects of physiological doses of UVR (UVA + UVB) on quiescent melanocytes in ...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12888

    authors: Sha J,Arbesman J,Harter ML

    更新日期:2020-09-01 00:00:00

  • NRASQ61K melanoma tumor formation is reduced by p38-MAPK14 activation in zebrafish models and NRAS-mutated human melanoma cells.

    abstract::Oncogenic BRAF and NRAS mutations drive human melanoma initiation. We used transgenic zebrafish to model NRAS-mutant melanoma, and the rapid tumor onset allowed us to study candidate tumor suppressors. We identified P38α-MAPK14 as a potential tumor suppressor in The Cancer Genome Atlas melanoma cohort of NRAS-mutant m...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12925

    authors: Banik I,Cheng PF,Dooley CM,Travnickova J,Merteroglu M,Dummer R,Patton EE,Busch-Nentwich EM,Levesque MP

    更新日期:2020-09-10 00:00:00

  • Tyrosinase-catalyzed metabolism of rhododendrol (RD) in B16 melanoma cells: production of RD-pheomelanin and covalent binding with thiol proteins.

    abstract::RS-4-(4-Hydroxyphenyl)-2-butanol (rhododendrol, RD) was reported to induce leukoderma of the skin. To explore the mechanism underlying that effect, we previously showed that oxidation of RD with mushroom tyrosinase produces RD-quinone, which is converted to secondary quinone products, and we suggested that those quino...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12363

    authors: Ito S,Okura M,Nakanishi Y,Ojika M,Wakamatsu K,Yamashita T

    更新日期:2015-05-01 00:00:00

  • Pharmacologic suppression of MITF expression via HDAC inhibitors in the melanocyte lineage.

    abstract::Melanoma incidence continues to rise at an alarming rate while effective systemic therapies remain very limited. Microphthalmia-associated transcription factor (MITF) is required for development of melanocytes and is an amplified oncogene in a fraction of human melanomas. Microphthalmia-associated transcription factor...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/j.1755-148X.2008.00480.x

    authors: Yokoyama S,Feige E,Poling LL,Levy C,Widlund HR,Khaled M,Kung AL,Fisher DE

    更新日期:2008-08-01 00:00:00

  • Osteoblasts contribute to a protective niche that supports melanoma cell proliferation and survival.

    abstract::Melanoma is the deadliest form of skin cancer; a primary driver of this high level of morbidity is the propensity of melanoma cells to metastasize. When malignant tumours develop distant metastatic lesions the new local tissue niche is known to impact on the biology of the cancer cells. However, little is known about ...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12812

    authors: Ferguson J,Wilcock DJ,McEntegart S,Badrock AP,Levesque M,Dummer R,Wellbrock C,Smith MP

    更新日期:2020-01-01 00:00:00

  • Pigmentation PAX-ways: the role of Pax3 in melanogenesis, melanocyte stem cell maintenance, and disease.

    abstract::Transcription factors initiate programs of gene expression and are catalysts in downstream molecular cascades that modulate a variety of cellular processes. Pax3 is a transcription factor that is important in the melanocyte and influences melanocytic proliferation, resistance to apoptosis, migration, lineage specifici...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章,评审

    doi:10.1111/j.1755-148X.2008.00514.x

    authors: Kubic JD,Young KP,Plummer RS,Ludvik AE,Lang D

    更新日期:2008-12-01 00:00:00

  • Decreased regulatory T-cells and CD4(+) /CD8(+) ratio correlate with disease onset and progression in patients with generalized vitiligo.

    abstract::The aim of present study was to evaluate CD4(+) /CD8(+) ratio and CD4(+) CD25(hi) FoxP3(+) Tregs in GV patients with reference to their effect on disease onset and progression. Flow cytometry was used for determination of CD4(+) /CD8(+) ratio and Tregs in 82 patients and 50 controls. CD8(+) T-cell counts were signific...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12105

    authors: Dwivedi M,Laddha NC,Arora P,Marfatia YS,Begum R

    更新日期:2013-07-01 00:00:00

  • Increasing the complexity: new genes and new types of albinism.

    abstract::Albinism is a rare genetic condition globally characterized by a number of specific deficits in the visual system, resulting in poor vision, in association with a variable hypopigmentation phenotype. This lack or reduction in pigment might affect the eyes, skin, and hair (oculocutaneous albinism, OCA), or only the eye...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章,评审

    doi:10.1111/pcmr.12167

    authors: Montoliu L,Grønskov K,Wei AH,Martínez-García M,Fernández A,Arveiler B,Morice-Picard F,Riazuddin S,Suzuki T,Ahmed ZM,Rosenberg T,Li W

    更新日期:2014-01-01 00:00:00

  • Is it time to test biguanide metformin in the treatment of melanoma?

    abstract::Metformin is the most widely used antidiabetic drug that belongs to the biguanide class. It is very well tolerated and has the major clinical advantage of not inducing hypoglycemia. Metformin decreases hepatic glucose production via a mechanism requiring liver kinase B1, which controls the metabolic checkpoint, AMP-ac...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章,评审

    doi:10.1111/pcmr.12267

    authors: Cerezo M,Tomic T,Ballotti R,Rocchi S

    更新日期:2015-01-01 00:00:00

  • Oculocutaneous albinism type 1: link between mutations, tyrosinase conformational stability, and enzymatic activity.

    abstract::Oculocutaneous albinism type 1 (OCA1) is an autosomal recessive disorder caused by mutations in the tyrosinase gene. Two subtypes of OCA1 have been described: severe OCA1A with complete absence of tyrosinase activity and less severe OCA1B with residual tyrosinase activity. Here, we characterize the recombinant human t...

    journal_title:Pigment cell & melanoma research

    pub_type: 杂志文章

    doi:10.1111/pcmr.12546

    authors: Dolinska MB,Kus NJ,Farney SK,Wingfield PT,Brooks BP,Sergeev YV

    更新日期:2017-01-01 00:00:00