Abstract：
OBJECTIVE:To identify the effect of alterations in physical parameters such as oxygen and pH on processes associated with cellular redox balance in osteoarthritic chondrocytes. METHOD:Human osteoarthritic chondrocytes (HOAC) were isolated from total knee arthroplasty samples and cultured in 3-D alginate beads in four different oxygen tensions (<1%, 2%, 5% and 21% O2), at pH 7.2 and 6.2 and in the presence or absence of 10 ng/ml, interleukin-1β (IL-1β). Cell viability, media glycosaminoglycan (GAG) levels, media nitrate/nitrate levels, active matrix metalloproteinase (MMP)-13 and intracellular adenosine triphosphate (ATPi) were measured over a 96-h time course. Intracellular reactive oxygen species (ROS), mitochondrial membrane potential, intracellular pH and reduced/oxidised glutathione (GSH/GSSG) were additionally measured after 48-h incubation under these experimental conditions. RESULTS:Hypoxia (2% O2) and anoxia (<1% O2), acidosis (pH 6.2) and 10 ng/ml IL-1β reduced HOAC cell viability and increased GAG media levels. Acidosis and IL-1β increased nitrite/nitrate release, but increases were moderate at 2% O2 and significantly reduced at <1% O2. ATPi was significantly reduced following hypoxia and anoxia and acidosis. At 48 h cellular ROS levels were increased by acidosis and IL-1β but reduced in hypoxia and anoxia. Mitochondrial membrane potential was reduced in low oxygen, acidosis and IL-1β. Anoxia also resulted in intracellular acidosis. GSH/GSSG ratio was reduced in low oxygen conditions, acidosis and IL-1β. CONCLUSIONS:This study shows that oxygen and pH affect elements of the redox system in HOAC including cellular anti-oxidants, mitochondrial membrane potential and ROS levels.
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Collins JA,Moots RJ,Winstanley R,Clegg PD,Milner PIdoi
10.1016/j.joca.2013.06.028subject
Has Abstractpub_date
2013-11-01 00:00:00pages
1790-8issue
11eissn
1063-4584issn
1522-9653pii
S1063-4584(13)00864-9journal_volume
21pub_type
杂志文章abstract：PURPOSE:Our aim was to introduce a simplified MRI instrument, Rapid OsteoArthritis MRI Eligibility Score (ROAMES), for defining structural eligibility of patients for inclusion in disease-modifying osteoarthritis drug trials using a tri-compartmental anatomic approach that enables stratification of knees into different...
journal_title：Osteoarthritis and cartilage
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更新日期：2020-01-01 00:00:00
abstract：:To highlight methodological challenges in the design and conduct of randomized trials of surgical interventions and to propose strategies for addressing these challenges. This paper focuses on three broad areas: enrollment; intervention; and assessment including implications for analysis. For each challenge raised in ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
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abstract：OBJECTIVE:Articular cartilage of the mandibular condyle has limited ability to regenerate itself after injury. This study was to investigate whether osteochondral defects in mandibular condyle could be repaired by NELL-1(NEL-like molecule-1)-modified autogenous bone marrow mesenchymal stem cells (BMMSCs) and poly lacti...
journal_title：Osteoarthritis and cartilage
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更新日期：2011-06-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2014.12.002
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abstract：OBJECTIVE:Cartilage swelling is an early event in osteoarthritis (OA). However, the response of chondrocytes to increased tissue hydration is unknown. This work studied the volume and morphology of living in situ human chondrocytes as a function of cartilage degeneration. METHODS:The tibial plateaus from knee joints o...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/s1063-4584(02)00369-2
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abstract：OBJECTIVE:The purpose of this study was to develop a long-term model of cartilage injury that could be used to compare the effects of radiofrequency energy (RFE) and mechanical debridement as a treatment. METHODS:Partial thickness fibrillation of patellar cartilage was created in 16 mature ponies. Three months after t...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2006.06.021
更新日期：2007-02-01 00:00:00
abstract：OBJECTIVE:To examine the effect of IL-1 beta-induced *NO and PGE(2)release by stimulated superficial and deep chondrocyte/agarose constructs subjected to mechanical compression. DESIGN:Chondrocyte sub-populations were seeded separately in agarose constructs and cultured unstrained, within a 24-well tissue culture plat...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/s1063-4584(03)00149-3
更新日期：2003-09-01 00:00:00
abstract：BACKGROUND:Arthroscopic partial meniscectomy (APM) is the most frequently performed orthopedic procedure. Functional outcomes of APM are variable, particularly among patients with underlying knee osteoarthritis. While most patients undergoing APM have knee magnetic resonance imaging (MRI) performed preoperatively, the ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2005.11.014
更新日期：2006-05-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2014.11.021
更新日期：2015-03-01 00:00:00
abstract：BACKGROUND:To investigate changes in knee 3D bone shape over the first 5 years after acute anterior cruciate ligament (ACL) injury in participants of the randomized controlled KANON-trial. METHODS:Serial MR images over 5 years from 121 young (32 women, mean age 26.1 years) adults with an acute ACL tear in a previously...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,随机对照试验
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更新日期：2019-04-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2013.09.005
更新日期：2013-12-01 00:00:00
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journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2003.09.002
更新日期：2004-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the thickness of cartilage at the posterior aspect of the medial and lateral condyle in Osteoarthritis (OA) knees compared to non-OA knees using computed tomography arthrography (CTA). DESIGN:535 consecutive knee CTAs (mean patient age = 48.7 ± 16.0; 286 males), were retrospectively analyzed. Kne...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2014.08.017
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVE:To examine the effect of beta-aminopropionitrile (BAPN), an inhibitor of lysyl oxidase, on growth and remodeling of immature articular cartilage in vitro. DESIGN:Immature bovine articular cartilage explants from the superficial and middle layers were cultured for 13 days in serum-containing medium with or wi...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2007.05.019
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVES:To address the hypothesis that different types of established osteoarthritis (OA) pain behaviours have associations with different aspects of articular pathology, we investigated the relationship between structural knee joint pathology and pain behaviour following injection of a low vs a high dose of monosod...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2016.06.012
更新日期：2016-11-01 00:00:00
abstract：OBJECTIVE:The purpose of the current study was to investigate the feasibility of applying defect-size femoral implants for the treatment of localized cartilage defects in a 1-year follow-up model. METHODS:In 13 goats, a medial femoral condyle defect was created in both knees. Defects were randomly treated by immediate...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2009.10.009
更新日期：2010-03-01 00:00:00
abstract：AIM:The goal of this study was to determine whether or not the intraarticular administration of hyaluronic acid can improve functional parameters, such as isokinetic muscle strength or total work and clinical test results in patients with osteoarthritis (OA) of the knee. METHOD:As part of a prospective, controlled stu...
journal_title：Osteoarthritis and cartilage
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1053/joca.2002.0815
更新日期：2002-09-01 00:00:00
abstract：OBJECTIVE:The goal of this study was to characterize the proteoglycan (PG) production and processing by bone marrow stromal cells (BMSCs) within a tissue engineered construct. METHODS:Bovine BMSCs and articular chondrocytes (ACs) were isolated from an immature calf, seeded into agarose gels, and cultured up to 32 days...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2008.01.004
更新日期：2008-09-01 00:00:00
abstract：OBJECTIVE:To describe the reproducibility and validity of six different measurement techniques for knee subchondral bone mineral density (sBMD). METHODS:A consecutive sample of 50 male and female participants from a population-based longitudinal study had sBMD assessed using dual energy X-ray absorptiometry scans. Ant...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2008.04.012
更新日期：2008-12-01 00:00:00
abstract：OBJECTIVE:To compare measurement properties of the P4 pain scale, Western Ontario and McMaster Universities Arthritis Index pain subscale (WOMAC-pain), and Intermittent and Constant Osteoarthritis Pain (ICOAP) measure in patients with knee osteoarthritis (OA). DESIGN:A secondary analysis from a randomized controlled t...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.joca.2014.03.018
更新日期：2014-06-01 00:00:00
abstract：OBJECTIVE:To test the hypothesis that an association exists between the characteristics of the knee adduction moment and foot progression angle (FPA) in asymptomatic individuals and those with mild to moderate and severe knee osteoarthritis (OA). DESIGN:Fifty asymptomatic individuals, 46 patients with mild to moderate...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2007.11.012
更新日期：2008-08-01 00:00:00
abstract：OBJECTIVE:To investigate the mechanism of aggrecanolysis in interleukin-1 (IL-1)-treated cartilage tissue by examining the time course of aggrecan cleavages and the tissue and medium content of membrane type 4-matrix metalloproteinases (MT4-MMP) and a disintegrin and metalloproteinase with thrombospondin type I motifs ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2004.10.023
更新日期：2005-04-01 00:00:00
abstract：OBJECTIVE:Osteoarthritis (OA) is a debilitating chronic condition requiring long-term treatment of pain and functional impairment. Our objective was to characterize studies addressing management of OA-related pain with respect to the breadth of interventions, trial duration and size, outcome measures, and funding sourc...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,meta分析
doi：10.1016/j.joca.2013.04.013
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVE:The aim of the present study was to determine if the electroporation-mediated gene transfer of SOX trio enhances the chondrogenic potential of adipose stem cells (ASCs). DESIGN:ASCs were transfected with SOX trio genes using an electroporation technique and cultured for 3 weeks. The pellets were analyzed for...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2011.01.005
更新日期：2011-04-01 00:00:00
abstract：OBJECTIVE:Macromolecules of the articular cartilage extracellular matrix released into synovial fluid, blood, or urine can serve as potentially useful biomarkers of the severity of osteoarthritis (OA). Biomechanical factors play an important role in OA pathogenesis, yet their influence on biomarker production is not we...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2005.07.003
更新日期：2005-12-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to evaluate the morphological changes of the lateral meniscus in end-stage lateral compartment osteoarthritis (OA) of the knee. METHODS:One hundred fifty-eight knee joints from 133 patients that subsequently underwent total knee joint arthroplasty from January 2008 to December 2009 ...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2011.11.005
更新日期：2012-02-01 00:00:00
abstract：OBJECTIVE:This narrative review covers original research publications related to imaging advancements in osteoarthritis (OA) published in the English language between 1st April 2016 and 30th April 2017. METHODS:Relevant human studies (excluding pre-clinical and in vitro studies), were searched and selected from PubMed...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章,评审
doi：10.1016/j.joca.2018.01.007
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:To describe the construction and preliminary validation of a new population-based microsimulation model developed to analyse the health and economic burden and cost-effectiveness of treatments for knee osteoarthritis (OA) in New Zealand (NZ). METHOD:We developed the New Zealand Management of Osteoarthritis (...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2018.01.004
更新日期：2018-04-01 00:00:00
abstract：OBJECTIVE:According to prevailing consensus, patients with mechanical symptoms are those considered to most likely benefit from arthroscopic surgery. The aim of this study was to determine the value of using patients' pre-operative self-reports of mechanical symptoms as a justification surgery in patients with degenera...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/j.joca.2016.03.013
更新日期：2016-08-01 00:00:00
abstract：OBJECTIVE:To validate quantitative magnetic resonance imaging (qMRI) for the assessment of cartilage volume and thickness in thin and curved cartilage layers, such as the shoulder. METHODS:Eight shoulder specimens from healthy individuals (aged 31-69 years) were investigated using a 3D gradient echo sequence with sele...
journal_title：Osteoarthritis and cartilage
pub_type： 杂志文章
doi：10.1016/s1063-4584(03)00077-3
更新日期：2003-07-01 00:00:00
