Abstract:
:Regulation of development and entry into sporulation is critical for fungi to ensure survival of unfavorable environmental conditions. Here we present an analysis of gene sets regulating sporulation in the homothallic ascomycete Ashbya gossypii. Deletion of components of the conserved pheromone/starvation MAP kinase cascades, e.g., STE11 and STE7, results in increased sporulation. In kar3 mutants sporulation is severely reduced, while deletion of KAR4 as well as of homologs of central Saccharomyces cerevisiae regulators of sporulation, IME1, IME2, IME4, and NDT80, abolishes sporulation in A. gossypii. Comparison of RNAseq transcript profiles of sporulation-deficient mutants identified a set of 67 down-regulated genes, most of which were up-regulated in the oversporulating ste12 mutant. One of these differentially expressed genes is an endoglucanase encoded by ENG2. We found that Eng2p promotes hyphal fragmentation as part of the developmental program of sporulation, which generates single-celled sporangia. Sporulation-deficient strains are arrested in their development but form sporangia. Supply of new nutrients enabled sporangia to return to hyphal growth, indicating that these cells are not locked in meiosis. Double-strand break (DSB) formation by Spo11 is apparently not required for sporulation; however, the absence of DMC1, which repairs DSBs in S. cerevisiae, results in very poor sporulation in A. gossypii. We present a comprehensive analysis of the gene repertoire governing sporulation in A. gossypii and suggest an altered regulation of IME1 expression compared to S. cerevisiae.
journal_name
Geneticsjournal_title
Geneticsauthors
Wasserstrom L,Lengeler KB,Walther A,Wendland Jdoi
10.1534/genetics.113.151019subject
Has Abstractpub_date
2013-09-01 00:00:00pages
87-99issue
1eissn
0016-6731issn
1943-2631pii
genetics.113.151019journal_volume
195pub_type
杂志文章相关文献
GENETICS文献大全abstract::The complete nucleotide sequence of the 14,771-bp-long mitochondrial (mt) DNA of a urochordate (Chordata)-the ascidian Halocynthia roretzi-was determined. All the Halocynthia mt-genes were found to be located on a single strand, which is rich in T and G rather than in A and C. Like nematode and Mytilus edulis mtDNAs, ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Mutations affecting the self-incompatibility response of Nicotiana alata were generated by irradiation. Mutants in the M1 generation were selected on the basis of pollen tube growth through an otherwise incompatible pistil. Twelve of the 18 M1 plants obtained from the mutagenesis screen were self-compatible. Eleven se...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::During development signaling pathways coordinate cell fates and regulate the choice between cell survival or programmed cell death. The well-conserved Wingless/Wnt pathway is required for many developmental decisions in all animals. One transducer of the Wingless/Wnt signal is Armadillo/beta-catenin. Drosophila Armadi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::A method for the estimation of population dynamic history from sequence data is described and used to investigate the past population dynamics of HIV-1 subtypes A and B. Using both gag and env gene alignments the effective population size of each subtype is estimated and found to be surprisingly small. This may be a r...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
abstract::Parentage analysis is an important method that is used widely in zoological and ecological studies. Current mathematical models of parentage analyses usually assume that a population has a uniform genetic structure and that mating is panmictic. In a natural population, the geographic or social structure of a populatio...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301592
更新日期:2018-12-01 00:00:00
abstract::We have isolated a new class of deletion mutants of phage lambda that extend from the prophage attachment site, att, into the gam and cIII genes. In this respect they are similar to certain of the lambda pbio transducing phage, but they differ in having a low burst size and in forming minute plaques. Lytically grown s...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-02-01 00:00:00
abstract::Stress-induced mutagenesis is a collection of mechanisms observed in bacterial, yeast, and human cells in which adverse conditions provoke mutagenesis, often under the control of stress responses. Control of mutagenesis by stress responses may accelerate evolution specifically when cells are maladapted to their enviro...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.100735
更新日期:2009-05-01 00:00:00
abstract::Knowing the amount of DNA polymorphism is essential to understand the mechanism of maintaining DNA polymorphism in a natural population. The amount of DNA polymorphism can be measured by the average number of nucleotide differences per site (pi), the proportion of segregating (polymorphic) site (s) and the minimum num...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::The wild-type Salmonella typhimurium strain LT2 contains six copies of the insertion sequence element IS200 which is unique to Salmonella. We have determined the chromosomal locations of all six copies of IS200 in strain LT2. This was done by mapping the positions of Tn10 elements inserted near each copy of IS200. Suc...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1983-12-01 00:00:00
abstract::The ade6-M26 mutation of the fission yeast Schizosaccharomyces pombe creates a meiotic recombination hotspot that elevates ade6 intragenic recombination approximately 10-15-fold. A heptanucleotide sequence including the M26 point mutation is required but not sufficient for hotspot activity. We studied the effects of p...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::The presence of large genomic regions with suppressed recombination (SR) is a key shared property of some sex- and mating-type determining (mat) chromosomes identified to date in animals, plants, and fungi. Why such regions form and how they evolve remain central questions in evolutionary genetics. The smut fungus Mic...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.171702
更新日期:2015-03-01 00:00:00
abstract::Evolutionary rescue describes a situation where adaptive evolution prevents the extinction of a population facing a stressing environment. Models of evolutionary rescue could in principle be used to predict the level of stress beyond which extinction becomes likely for species of conservation concern, or, conversely, ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.300908
更新日期:2018-05-01 00:00:00
abstract::Detecting and quantifying the differences in individual genomes (i.e., genotyping), plays a fundamental role in most modern bioinformatics pipelines. Many scientists now use reduced representation next-generation sequencing (NGS) approaches for genotyping. Genotyping diploid individuals using NGS is a well-studied fie...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301468
更新日期:2018-11-01 00:00:00
abstract::Selection of Gl-Sb coupling heterozygotes was carried out for more than one hundred generations commencing with six independent lines drawn from a common base population. Population sizes were eight, sixteen and forty-eight parents per generation. The effect of natural selection on recombination value was measured by ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1972-03-01 00:00:00
abstract::Low-coverage next-generation sequencing methodologies are routinely employed to genotype large populations. Missing data in these populations manifest both as missing markers and markers with incomplete allele recovery. False homozygous calls at heterozygous sites resulting from incomplete allele recovery confound man...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.182071
更新日期:2016-02-01 00:00:00
abstract::Mobile P elements in Drosophila melanogaster cause hybrid dysgenesis if their mobility is not repressed. One type of repression, termed P cytotype, is a complex interaction between chromosomes carrying P elements and cytoplasm and is transmitted through the cytoplasm only of females. Another type of repression is foun...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::We formalize the use of allele frequency and geographic information for the construction of gene trees at the intraspecific level and extend the concept of evolutionary parsimony to molecular variance parsimony. The central principle is to consider a particular gene tree as a variable to be optimized in the estimation...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::The distinction between deleterious, neutral, and adaptive mutations is a fundamental problem in the study of molecular evolution. Two significant quantities are the fraction of DNA variation in natural populations that is deleterious and destined to be eliminated and the fraction of fixed differences between species ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
abstract::The decrease of meiotic exchanges (crossing over and conversion) in two mutants of Sordaria macrospora correlated strongly with a reduction of chiasmata and of both types of "recombination nodules." Serial section reconstruction electron microscopy was used to compare the synapsis pattern of meiotic prophase I in wild...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
abstract::We present a Bayesian method for characterizing the mating system of populations reproducing through a mixture of self-fertilization and random outcrossing. Our method uses patterns of genetic variation across the genome as a basis for inference about reproduction under pure hermaphroditism, gynodioecy, and a model de...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.179093
更新日期:2015-11-01 00:00:00
abstract::We show how to incorporate fluctuations of the recombination rate along the chromosome into standard gene-genealogical models for the decorrelation of gene histories. This enables us to determine how small-scale fluctuations (Poissonian hot-spot model) and large-scale variations (Kong et al. 2002) of the recombination...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.103.018002
更新日期:2005-02-01 00:00:00
abstract::We subjected the genes encoding the 19.3-, 21.3c-, and 51-kDa iron-sulfur subunits of respiratory chain complex I from Neurospora crassa to site-directed mutagenesis to mimic mutations in human complex I subunits associated with mitochondrial diseases. The V135M substitution was introduced into the 19.3-kDa cDNA, the ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.041517
更新日期:2005-09-01 00:00:00
abstract::Significant correlations between allelic frequencies and environmental variables in a number of insect species have been demonstrated by multivariate techniques. Since many environmental variables show a strong relationship to geographic location and since gene flow between populations can also produce patterns of gen...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-05-01 00:00:00
abstract::Microarray experiments have been used recently in genetical genomics studies, as an additional tool to understand the genetic mechanisms governing variation in complex traits, such as for estimating heritabilities of mRNA transcript abundances, for mapping expression quantitative trait loci, and for inferring regulato...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.106.057281
更新日期:2006-10-01 00:00:00
abstract::Mutations in the SPT10 and SPT21 genes were originally isolated as suppressors of Ty and LTR (delta) insertion mutations in Saccharomyces cerevisiae, and the genes were shown to be required for normal transcription at a number of loci in yeast. Now we have cloned, sequenced, mapped and mutagenized SPT10 and SPT21. Sin...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::High-throughput sequencing and bioinformatic techniques have enhanced classical genetic analysis and are essential methods for geneticists. Tsukamoto and colleagues use numerous genomic and bioinformatics methods to explore the role of ribonucleoprotein complexes in regulating oocyte meiotic maturation, which is the t...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301139
更新日期:2018-07-01 00:00:00
abstract::An association was found earlier between the Ep(1) gene locus coding for an endopeptidase and the endosperm color gene Y(1) on chromosome 6 of Zea mays. By employing primary trisomics we have unequivocally placed the Ep(1) gene on chromosome 6, closely linked to the Y(1) locus. Additionally we describe new alleles at ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-08-01 00:00:00
abstract::The rate of microsatellite mutation is dependent upon both the allele length and the repeat motif, but the exact nature of this relationship is still unknown. We analyzed data on the inheritance of human Y-chromosomal microsatellites in father-son duos, taken from 24 published reports and comprising 15,285 directly ob...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.132308
更新日期:2011-12-01 00:00:00
abstract::The experimental population genetics of Y-chromosome drive in Drosophila melanogaster is approximated by studying the behavior of T(Y;2),SD lines. These exhibit "pseudo-Y" drive through the effective coupling of the Y chromosome to the second chromosome meiotic drive locus, Segregation distorter (SD). T(Y;2),SD males ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
abstract::Dispersal is a crucial factor in natural evolution, since it determines the habitat experienced by any population and defines the spatial scale of interactions between individuals. There is compelling evidence for systematic differences in dispersal characteristics within the same population, i.e., genotype-dependent ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.193946
更新日期:2017-01-01 00:00:00