Abstract:
:We report a salvage pathway in Gram-negative bacteria that bypasses de novo biosynthesis of UDP N-acetylmuramic acid (UDP-MurNAc), the first committed peptidoglycan precursor, and thus provides a rationale for intrinsic fosfomycin resistance. The anomeric sugar kinase AmgK and the MurNAc α-1-phosphate uridylyl transferase MurU, defining this new cell wall sugar-recycling route in Pseudomonas putida, were characterized and engineered into Escherichia coli, channeling external MurNAc directly to peptidoglycan biosynthesis.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Gisin J,Schneider A,Nägele B,Borisova M,Mayer Cdoi
10.1038/nchembio.1289subject
Has Abstractpub_date
2013-08-01 00:00:00pages
491-3issue
8eissn
1552-4450issn
1552-4469pii
nchembio.1289journal_volume
9pub_type
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