Mutual effect of subcutaneously transplanted human adipose-derived stem cells and pancreatic islets within fibrin gel.

Abstract:

:While subcutaneous tissue has been proposed as a potential site for pancreatic islet transplantation, concern remains that the microvasculature of subcutaneous tissue is too poor to support transplanted islets. In an effort to overcome this limitation, we evaluated whether fibrin gel with human adipose-derived stem cells (hADSCs) and rat pancreatic islets could cure diabetes mellitus when transplanted into the subcutaneous space of diabetic mice. Subcutaneously co-transplanted islets and hADSCs showed normalization of the diabetic recipient's blood glucose levels. The result was enhanced by co-treatment of fibroblast growth factor-2 (FGF2) in the fibrin gel. The hADSCs enhanced islet viability after transplantation by secreting various growth factors that can protect islets from hypoxic damage. Afterward, hADSCs could maintain islet viability by recruiting new microvasculature nearby the transplanted islets via overexpression of vascular endothelial growth factor (VEGF). The hADSCs did not directly differentiate into endothelial cells (no detection of biomarkers of human endothelial cells), but showed evidence of differentiation toward insulin-secreting cells (detection of human insulin). Mice receiving islet transplantation alone did not become normoglycemic. Collectively, co-transplantation of fibrin gel with islets and hADSCs will expand the indications for islet transplant therapy and the potential clinical application of cell-based therapy.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Bhang SH,Jung MJ,Shin JY,La WG,Hwang YH,Kim MJ,Kim BS,Lee DY

doi

10.1016/j.biomaterials.2013.06.018

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

7247-56

issue

30

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(13)00704-7

journal_volume

34

pub_type

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