Enrichment of denitrifying anaerobic methane oxidizing microorganisms.

Abstract:

:The microorganisms responsible for anaerobic oxidation of methane (AOM) coupled to denitrification have not been clearly elucidated. Three recent publications suggested it can be achieved by a denitrifying bacterium with or without the involvement of anaerobic methanotrophic archaea. A key factor limiting the progress in this research field is the shortage of enrichment cultures performing denitrifying anaerobic methane oxidation (DAMO). In this study, DAMO cultures were enriched from mixed inoculum including sediment from a freshwater lake, anaerobic digester sludge and return activated sludge from a sewage treatment plant. Two reactors, operated at 35°C and at 22°C, respectively, showed simultaneous methane oxidation and nitrate reduction after several months of operation. Analysis of 16S rRNA gene clone libraries from the 35°C enrichment showed the presence of an archaeon closely related to other DAMO archaea and a dominated bacterium belonging to the yet uncultivated NC10 phylum. This culture preferred nitrite to nitrate as the electron acceptor. The present study suggests that the archaea are rather methanotrophs than methanogens. The highest denitrification rate achieved was 2.35 mmol NO3 (-) -N gVSS(-1)  day(-1) . The culture enriched at 22°C contained the same NC10 bacterium observed in the culture enriched at 35°C but no archaea.

journal_name

Environ Microbiol Rep

authors

Hu S,Zeng RJ,Burow LC,Lant P,Keller J,Yuan Z

doi

10.1111/j.1758-2229.2009.00083.x

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

377-84

issue

5

issn

1758-2229

journal_volume

1

pub_type

杂志文章
  • Targeted and shotgun metagenomic approaches provide different descriptions of dryland soil microbial communities in a manipulated field study.

    abstract::The extent to which different sequence-based approaches describe environmental microbial communities in comparative studies is an important consideration when deriving inferences from ecological studies. The ability of a targeted metagenomic approach [small subunit (SSU) rRNA pyrosequencing] and shotgun metagenome app...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2012.00328.x

    authors: Steven B,Gallegos-Graves LV,Starkenburg SR,Chain PS,Kuske CR

    更新日期:2012-04-01 00:00:00

  • Marine bacterial communities are resistant to elevated carbon dioxide levels.

    abstract::It is well established that the release of anthropogenic-derived CO2 into the atmosphere will be mainly absorbed by the oceans, with a concomitant drop in pH, a process termed ocean acidification. As such, there is considerable interest in how changes in increased CO2 and lower pH will affect marine biota, such as bac...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12159

    authors: Oliver AE,Newbold LK,Whiteley AS,van der Gast CJ

    更新日期:2014-12-01 00:00:00

  • A novel alphaproteobacterium with a small genome identified from the digestive gland of multiple species of abalone.

    abstract::We identified an alphaproteobacterium in the digestive gland of the abalone species Haliotis discus hannai. This phylotype dominated our 16S rRNA clone libraries from the digestive gland of H. discus hannai. Diversity surveys revealed that this phylotype was associated with H. discus hannai and also in another host sp...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12845

    authors: Huang Z,Petersen JM,Martijn J,Ettema TJG,Shao Z

    更新日期:2020-08-01 00:00:00

  • Changes in the rumen microbiome and metabolites reveal the effect of host genetics on hybrid crosses.

    abstract::The rumen microbiota plays important roles in nutrient metabolism and absorption of the host. However, it is poorly understood how host genetic variation shapes the community structure of the rumen microbiota and its metabolic phenotype. Here, we used sika deer (Cervus nippon) and elk (Cervus elaphus) to produce the f...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12482

    authors: Li Z,Wright AG,Si H,Wang X,Qian W,Zhang Z,Li G

    更新日期:2016-12-01 00:00:00

  • Is microbial terroir related to geographic distance between vineyards?

    abstract::While there are substantial studies suggesting that characteristics of wine are related to regional microbial community composition (microbial terroir), there has been little discussion about what factors affect variation in regional microbial community composition. In this study, we compared the microbial community c...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12589

    authors: Miura T,Sánchez R,Castañeda LE,Godoy K,Barbosa O

    更新日期:2017-12-01 00:00:00

  • High cyanobacterial nifH gene diversity in Arctic seawater and sea ice brine.

    abstract::Although cyanobacterial diazotrophs are common in Arctic terrestrial and freshwater habitats, they have been assumed to be absent from Arctic marine habitats. We report here a high diversity of cyanobacterial nifH genes in Fram Strait and the Greenland Sea. The nifH gene encodes the iron protein of the nitrogenase enz...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2012.00343.x

    authors: Díez B,Bergman B,Pedrós-Alió C,Antó M,Snoeijs P

    更新日期:2012-06-01 00:00:00

  • Bacterial role in pine wilt disease development - review and future perspectives.

    abstract::Mutualistic and beneficial relationships between nematodes and bacteria are highly present in nature, mostly occurring because of nutritional dependence and pathogen protection, and intrinsically related with the environment, the ecological conditions and the nematode life stages. Thirty-four years have passed since t...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章,评审

    doi:10.1111/1758-2229.12202

    authors: Nascimento FX,Hasegawa K,Mota M,Vicente CS

    更新日期:2015-02-01 00:00:00

  • Photoautotrophic-heterotrophic biofilm communities: a laboratory incubator designed for growing axenic diatoms and bacteria in defined mixed-species biofilms.

    abstract::Biofilm communities in the euphotic zone of aquatic habitats comprise photoautotrophic microorganisms, such as diatoms, green algae and cyanobacteria, which produce the organic carbon that fuels the life of a heterotrophic contingent of microorganisms, mostly bacteria. Such photoautotrophic-heterotrophic mixed-species...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2011.00315.x

    authors: Buhmann M,Kroth PG,Schleheck D

    更新日期:2012-02-01 00:00:00

  • Enigmatic reticulated filaments in subsurface granite.

    abstract::In the last few years, geomicrobiologists have focused their researches on the nature and origin of enigmatic reticulated filaments reported in modern and fossil samples from limestone caves and basalt lava tubes. Researchers have posed questions on these filaments concerning their nature, origin, chemistry, morpholog...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2012.00375.x

    authors: Miller AZ,Hernández-Mariné M,Jurado V,Dionísio A,Barquinha P,Fortunato E,Afonso MJ,Chaminé HI,Saiz-Jimenez C

    更新日期:2012-12-01 00:00:00

  • Microdiversity shapes the traits, niche space, and biogeography of microbial taxa.

    abstract::With rapidly improving sequencing technologies, scientists have recently gained the ability to examine diverse microbial communities at high genomic resolution, revealing that both free-living and host-associated microbes partition their environment at fine phylogenetic scales. This 'microdiversity,' or closely relate...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章,评审

    doi:10.1111/1758-2229.12523

    authors: Larkin AA,Martiny AC

    更新日期:2017-04-01 00:00:00

  • The nitrogen regulator PipX acts in cis to prevent operon polarity.

    abstract::Cyanobacteria, phototrophic organisms performing oxygenic photosynthesis, must adapt their metabolic processes to important environmental challenges, like those imposed by the succession of days and nights. Not surprisingly, certain regulatory proteins are found exclusively in this phylum. One of these unique factors,...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12688

    authors: Cantos R,Labella JI,Espinosa J,Contreras A

    更新日期:2019-08-01 00:00:00

  • Genetic dissection of a motility-associated c-di-GMP signalling protein of Pseudomonas putida.

    abstract::Lack of the Pseudomonas putida PP2258 protein or its overexpression results in defective motility on solid media. The PP2258 protein is tripartite, possessing a PAS domain linked to two domains associated with turnover of c-di-GMP - a cyclic nucleotide that controls the switch between motile and sessile lifestyles. Th...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12045

    authors: Österberg S,Åberg A,Herrera Seitz MK,Wolf-Watz M,Shingler V

    更新日期:2013-08-01 00:00:00

  • Evidence for the biogenic origin of manganese-enriched layers in Lake Superior sediments.

    abstract::Manganese (Mn) and iron (Fe)-enriched sediment layers were discovered in Lake Superior within, above and below the oxic-anoxic interface. While the role of bacteria in redox reactions with Mn is known to be significant, little information exists about indigenous microbial communities in many freshwater environments. T...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12364

    authors: Palermo C,Dittrich M

    更新日期:2016-04-01 00:00:00

  • The versatile in situ gene expression of an Epsilonproteobacteria-dominated biofilm from a hydrothermal chimney.

    abstract::The Epsilonproteobacteria, including members of the genus Sulfurovum, are regarded as important primary producers in hydrothermal systems. However, their in situ gene expression in this habitat has so far not been investigated. We report a metatranscriptomic analysis of a Sulfurovum-dominated biofilm from one of the c...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12016

    authors: Dahle H,Roalkvam I,Thorseth IH,Pedersen RB,Steen IH

    更新日期:2013-04-01 00:00:00

  • Staphylococcus aureus virulence and metabolism are dramatically affected by Lactococcus lactis in cheese matrix.

    abstract::In complex environments such as cheeses, the lack of relevant information on the physiology and virulence expression of pathogenic bacteria and the impact of endogenous microbiota has hindered progress in risk assessment and control. Here, we investigated the behaviour of Staphylococcus aureus, a major foodborne patho...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2010.00230.x

    authors: Cretenet M,Nouaille S,Thouin J,Rault L,Stenz L,François P,Hennekinne JA,Piot M,Maillard MB,Fauquant J,Loubière P,Le Loir Y,Even S

    更新日期:2011-06-01 00:00:00

  • Stage 0 sporulation gene A as a molecular marker to study diversity of endospore-forming Firmicutes.

    abstract::In this study, we developed and validated a culture-independent method for diversity surveys to specifically detect endospore-forming Firmicutes. The global transcription regulator of sporulation (spo0A) was identified as a gene marker for endospore-forming Firmicutes. To enable phylogenetic classification, we designe...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12094

    authors: Wunderlin T,Junier T,Roussel-Delif L,Jeanneret N,Junier P

    更新日期:2013-12-01 00:00:00

  • Oestrogenicity of prenylflavonoids from hops: activation of pro-oestrogens by intestinal bacteria.

    abstract::For many centuries, hops (Humulus lupulus L.) have been used as essential ingredient in beers, providing the typical bitterness and hoppy flavour. However, the last few years the plant has gained increasing attention as a source of prenylflavonoids and in 1999, 8-prenylnaringenin (8-PN) was identified as the most pote...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2009.00011.x

    authors: Possemiers S,Verstraete W

    更新日期:2009-04-01 00:00:00

  • Isolation and genetic characterization of an improved benzene-tolerant mutant of Pseudomonas putida S12.

    abstract::Pseudomonas putida S12.49, a mutant stain of P. putida S12 that tolerates up to 20 mM benzene, was obtained by evolutionary selection. The genetic basis for the strongly enhanced benzene tolerance was investigated by proteome and transcriptome analysis. Indications were found that the highly benzene-tolerant phenotype...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2010.00172.x

    authors: Volkers RJ,Ballerstedt H,De Winde JH,Ruijssenaars HJ

    更新日期:2010-06-01 00:00:00

  • Subtle bacterioplankton community responses to elevated CO2 and warming in the oligotrophic South Pacific gyre.

    abstract::Bacterioplankton play a critical role in primary production, carbon cycling, and nutrient cycling in the oligotrophic ocean. To investigate the effect of elevated CO2 and warming on the composition and function of bacterioplankton communities in oligotrophic waters, we performed two trace-metal clean deck board incuba...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12844

    authors: Allen R,Hoffmann LJ,Law CS,Summerfield TC

    更新日期:2020-08-01 00:00:00

  • Grazing of heterotrophic flagellates on viruses is driven by feeding behaviour.

    abstract::The trophic interactions between viruses, bacteria and protists play a crucial role in structuring microbial communities and regulating nutrient and organic matter flux. Here, we show that the impact on viral density by heterotrophic flagellates is related to their feeding behaviour (feeding on sedimented particles - ...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12119

    authors: Deng L,Krauss S,Feichtmayer J,Hofmann R,Arndt H,Griebler C

    更新日期:2014-08-01 00:00:00

  • Manganese is an additional cation that enhances colonial phase variation of Vibrio vulnificus.

    abstract::Vibrio vulnificus, an inhabitant of marine and estuarine environments around the world, is the leading cause of reported seafood-related deaths in the United States. Disease is caused by opaque colony-forming strains that produce capsular polysaccharide, loss of which results in an unencapsulated translucent phenotype...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12318

    authors: Kaluskar ZM,Garrison-Schilling KL,McCarter KS,Lambert B,Simar SR,Pettis GS

    更新日期:2015-10-01 00:00:00

  • KaiC family proteins integratively control temperature-dependent UV resistance in Methylobacterium extorquens AM1.

    abstract::KaiC protein is the pivotal component of the circadian clock in cyanobacteria. While KaiC family proteins are well-conserved throughout divergent phylogenetic lineages, studies of the physiological roles of KaiC proteins from other microorganisms have been limited. We examined the role of the KaiC proteins, KaiC1 and ...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12662

    authors: Iguchi H,Yoshida Y,Fujisawa K,Taga H,Yurimoto H,Oyama T,Sakai Y

    更新日期:2018-12-01 00:00:00

  • Contrasting microbial assemblages in adjacent water masses associated with the East Australian Current.

    abstract::Different oceanographic provinces host discrete microbial assemblages that are adapted to local physicochemical conditions. We sequenced and compared the metagenomes of two microbial communities inhabiting adjacent water masses in the Tasman Sea, where the recent strengthening of the East Australian Current (EAC) has ...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2012.00362.x

    authors: Seymour JR,Doblin MA,Jeffries TC,Brown MV,Newton K,Ralph PJ,Baird M,Mitchell JG

    更新日期:2012-10-01 00:00:00

  • Genomic distribution of B-vitamin auxotrophy and uptake transporters in environmental bacteria from the Chloroflexi phylum.

    abstract::Bacteria from the Chloroflexi phylum are dominant members of phototrophic microbial mat communities in terrestrial thermal environments. Vitamins of B group are key intermediates (precursors) in the biosynthesis of indispensable enzyme cofactors driving numerous metabolic processes in all forms of life. A genomics-bas...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12227

    authors: Rodionova IA,Li X,Plymale AE,Motamedchaboki K,Konopka AE,Romine MF,Fredrickson JK,Osterman AL,Rodionov DA

    更新日期:2015-04-01 00:00:00

  • Nitrate addition has minimal short-term impacts on greenland ice sheet supraglacial prokaryotes.

    abstract::Tropospheric nitrate levels are predicted to increase throughout the 21st century, with potential effects on terrestrial ecosystems, including the Greenland ice sheet (GrIS). This study considers the impacts of elevated nitrate concentrations on the abundance and composition of dominant bulk and active prokaryotic com...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12510

    authors: Cameron KA,Stibal M,Chrismas N,Box J,Jacobsen CS

    更新日期:2017-04-01 00:00:00

  • Ultrastructural analysis of spores from diverse Bacillales species isolated from Brazilian soil.

    abstract::Many species in the order Bacillales form a specialized cell type called a spore that is resistant to a range of environmental stresses. Transmission electron microscopy (TEM) reveals that the spore is comprised of a series of concentric shells, surrounding an interior compartment harbouring the spore DNA. The outermo...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12713

    authors: de Andrade Cavalcante D,De-Souza MT,de Orem JC,de Magalhães MIA,Martins PH,Boone TJ,Castillo JA,Driks A

    更新日期:2019-04-01 00:00:00

  • ddPCR applied on archived Continuous Plankton Recorder samples reveals long-term occurrence of class 1 integrons and a sulphonamide resistance gene in marine plankton communities.

    abstract::Antibiotic resistance is a rising threat for human health. Although in clinical settings and terrestrial environments the rise of antibiotic resistant bacteria is well documented, their dissemination and spread in the marine environment, covering almost two-thirds of the Earth's surface, is still poorly understood. In...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12665

    authors: Di Cesare A,Petrin S,Fontaneto D,Losasso C,Eckert EM,Tassistro G,Borello A,Ricci A,Wilson WH,Pruzzo C,Vezzulli L

    更新日期:2018-08-01 00:00:00

  • Assimilation of marine extracellular polymeric substances by deep-sea prokaryotes in the NW Mediterranean Sea.

    abstract::This study examined total uptake of extracellular polymeric substances (EPS) and glucose and the percentage of prokaryotic cells (Bacteria, Crenarchaea and Euryarchaea) consuming these compounds in the major water masses at the DYFAMED site (NW Mediterranean Sea). The potential assimilation rates of EPS at 10 m depth ...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2011.00285.x

    authors: Boutrif M,Garel M,Cottrell MT,Tamburini C

    更新日期:2011-12-01 00:00:00

  • Polar solar panels: Arctic and Antarctic microbiomes display similar taxonomic profiles.

    abstract::Solar panels located on high (Arctic and Antarctic) latitudes combine the harshness of the climate with that of the solar exposure. We report here that these polar solar panels are inhabited by similar microbial communities in taxonomic terms, dominated by Hymenobacter spp., Sphingomonas spp. and Ascomycota. Our resul...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12608

    authors: Tanner K,Martí JM,Belliure J,Fernández-Méndez M,Molina-Menor E,Peretó J,Porcar M

    更新日期:2018-02-01 00:00:00

  • Comparing effective population sizes of dominant marine alphaproteobacteria lineages.

    abstract::A fundamental question in marine microbial ecology is how microbes adapt to ocean environments. Although numerically dominant populations are typically considered more successful, higher census population sizes (Nc) do not equate directly to a greater capability for adaptation. Instead, effective population size (Ne) ...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12129

    authors: Luo H,Swan BK,Stepanauskas R,Hughes AL,Moran MA

    更新日期:2014-04-01 00:00:00