Abstract:
:Biofilm communities in the euphotic zone of aquatic habitats comprise photoautotrophic microorganisms, such as diatoms, green algae and cyanobacteria, which produce the organic carbon that fuels the life of a heterotrophic contingent of microorganisms, mostly bacteria. Such photoautotrophic-heterotrophic mixed-species biofilms have received little attention in biofilm research due to a lack of suitable pure-culture laboratory model systems. However, they offer important insight into microbial population dynamics and community interactions during a biofilm-developmental process that shapes highly structured, extremely well-adapted microbial landscapes. Here, we report on the development of a sterile incubation chamber for growing and monitoring axenic phototrophic biofilms, i.e. a sterilizable, illuminated, continuous-flow system for a routine work with pure cultures. The system has been designed to simulate the growth conditions in the shallow, littoral zone of aquatic habitats (horizontal surface, submerged in water, illuminated, aerated). Additional features of the concept include automated photometrical monitoring of biofilm density (as biofilm turbidity), analysis via confocal microscopy, direct harvesting of cells, and options to control illumination, flow velocity, and composition of culture fluid. The application of the system was demonstrated in growth experiments using axenic diatom biofilms, or axenic diatom biofilms co-cultivated with different bacterial strains isolated from epilithic biofilms of an oligotrophic freshwater lake.
journal_name
Environ Microbiol Repjournal_title
Environmental microbiology reportsauthors
Buhmann M,Kroth PG,Schleheck Ddoi
10.1111/j.1758-2229.2011.00315.xsubject
Has Abstractpub_date
2012-02-01 00:00:00pages
133-40issue
1issn
1758-2229journal_volume
4pub_type
杂志文章abstract::'Bacterial consortium' sensu lato applies to mutualism or syntrophy-based systems consisting of unrelated bacteria. Consortia of cyanobacteria have been preferentially studied on Anabaena epibioses; non-photosynthetic satellites of other filamentous or unicellular cyanobacteria were also considered although structure-...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12298
更新日期:2015-08-01 00:00:00
abstract::In this study, an aquaporin protein, Aqp1, in Cryptococcus neoformans, which can lead either saprobic or parasitic lifestyles and causes life-threatening fungal meningitis was identified and characterized. AQP1 expression was rapidly induced (via the HOG pathway) by osmotic or oxidative stress. In spite of such transc...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12527
更新日期:2017-06-01 00:00:00
abstract::Interpretation of bacteriohopanepolyol (BHP) biomarkers tracing microbiological processes in modern and ancient sediments relies on understanding environmental controls of production and preservation. BHPs from methanotrophs (35-aminoBHPs) were studied in methane-amended aerobic river-sediment incubations at different...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12570
更新日期:2017-10-01 00:00:00
abstract::Dual stable isotope probing has been used to infer rates of microbial biomass production and modes of carbon fixation. In order to validate this approach for assessing archaeal production, the methanogenic archaeon Methanosarcina barkeri was grown either with H2 , acetate or methanol with D2 O and 13 C-dissolved inorg...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12876
更新日期:2020-10-01 00:00:00
abstract::Pyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functio...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12545
更新日期:2017-08-01 00:00:00
abstract::Given the key role played by honey bees in almost all terrestrial ecosystems, maintaining bee populations in adequate sanitary conditions is crucial for these essential pollinators to continue their work. From the beginning of the 21st century, beekeepers have reported a progressive increase in the overwintering morta...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2011.00299.x
更新日期:2012-02-01 00:00:00
abstract::Copper is an essential element but in excess is highly toxic and therefore cytoplasmic levels must be tightly controlled. Member of the genus Burkholderia are highly resistant to various heavy metals and are often isolated from acidic soils where copper bioavailability is high. In this study, we employed transposon se...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12828
更新日期:2020-04-01 00:00:00
abstract::The flagellate protists in the hindgut of lower termites play an essential role in the digestion of lignocellulose. Most flagellate species are associated with host-specific symbionts from various bacterial lineages, which typically lack cultured representatives. In this study, we analyzed the genome of 'Candidatus An...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12451
更新日期:2016-10-01 00:00:00
abstract::Alexandrium catenella, a marine dinoflagellate responsible for harmful algal blooms (HABs), proliferates with greater frequency, distribution and intensity, in disturbed marine coastal ecosystems. The proteins secreted into seawater may play a crucial role in maintaining this dinoflagellate in these ecosystems, but th...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12764
更新日期:2019-08-01 00:00:00
abstract::Relationships fueled by sulfide between deep-sea invertebrates and bacterial symbionts are well known, yet the diverse overlapping factors influencing symbiont specificity are complex. For animals that obtain their symbionts from the environment, both host identity and geographic location can impact the ultimate symbi...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12909
更新日期:2020-11-16 00:00:00
abstract::The Epsilonproteobacteria, including members of the genus Sulfurovum, are regarded as important primary producers in hydrothermal systems. However, their in situ gene expression in this habitat has so far not been investigated. We report a metatranscriptomic analysis of a Sulfurovum-dominated biofilm from one of the c...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12016
更新日期:2013-04-01 00:00:00
abstract::Manganese (Mn) and iron (Fe)-enriched sediment layers were discovered in Lake Superior within, above and below the oxic-anoxic interface. While the role of bacteria in redox reactions with Mn is known to be significant, little information exists about indigenous microbial communities in many freshwater environments. T...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12364
更新日期:2016-04-01 00:00:00
abstract::Dehalococcoides mccartyi is a small, slow-growing bacterium of the phylum Chloroflexi that conserves energy using aliphatic and aromatic organohalides as electron acceptors, and hydrogen as sole electron donor. A recent study identified a protein complex in the membrane of strain CBDB1 comprising a Hup hydrogenase, a ...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12560
更新日期:2017-10-01 00:00:00
abstract::One-component systems (OCSs) and cytosolic two-component regulatory systems (TCSs) appear to share the same biological function, which consists in the transcriptional control in response to the cellular concentration of signal molecules. However, cytosolic TCSs as compared with OCSs represent a genetic and metabolic b...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2009.00020.x
更新日期:2009-06-01 00:00:00
abstract::Ectosymbiotic Nanoarchaeota live on the surface of diverse archaeal hosts. Despite being broadly distributed in global geothermal systems, only three Nanoarchaeota have been successfully co-cultivated with their hosts, and until now no nanoarchaeotal cultures or genomes have been described from deep-sea hydrothermal v...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12740
更新日期:2019-04-01 00:00:00
abstract::Pseudomonas putida KT2440 is an efficient colonizer of the rhizosphere of plants of agronomical and basic interest. We have demonstrated that KT2440 can protect the model plant Arabidopsis thaliana against infection by the phytopathogen Pseudomonas syringae pv. tomato DC3000. P. putida extracellular haem-peroxidase (P...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2009.00091.x
更新日期:2010-06-01 00:00:00
abstract::Cyanobacteria, phototrophic organisms performing oxygenic photosynthesis, must adapt their metabolic processes to important environmental challenges, like those imposed by the succession of days and nights. Not surprisingly, certain regulatory proteins are found exclusively in this phylum. One of these unique factors,...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12688
更新日期:2019-08-01 00:00:00
abstract::Bacteria from the Chloroflexi phylum are dominant members of phototrophic microbial mat communities in terrestrial thermal environments. Vitamins of B group are key intermediates (precursors) in the biosynthesis of indispensable enzyme cofactors driving numerous metabolic processes in all forms of life. A genomics-bas...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12227
更新日期:2015-04-01 00:00:00
abstract::Corals are inhabited by complex communities of microbes that affect their growth and survival. Several studies suggest that coral disease may be attributed to the success of vibrios in out-competing other bacteria in the mucus and tissues of corals. Vibrios utilize a variety of quorum sensing (QS) signal molecules to ...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2009.00122.x
更新日期:2010-02-01 00:00:00
abstract::Stromatolites are complex microbial mats that form lithified layers. Fossilized stromatolites are the oldest evidence of cellular life on Earth, dating back over 3.4 billion years. Modern stromatolites are relatively rare but may provide clues about the function and evolution of their ancient counterparts. In this stu...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12916
更新日期:2020-12-27 00:00:00
abstract::In field conditions, grapevine roots normally are colonized by arbuscular mycorrhizal fungi (AMF). However, little is published, from either morphological or molecular studies, on the species composition of these symbionts in production vineyards. The AMF biodiversity of two Piedmont vineyards (at Neive and Lessona), ...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2010.00160.x
更新日期:2010-08-01 00:00:00
abstract::Vibrio cholerae N-acetyl glucosamine-binding protein A (GbpA) is a chitin binding protein and a virulence factor involved in the colonization of human intestine. We investigated the distribution and genetic variations of gbpA in 488 V. cholerae strains of environmental and clinical origin, belonging to different serog...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2012.00356.x
更新日期:2012-08-01 00:00:00
abstract::In this study, we investigated quorum sensing regulation of virulence genes in Vibrio harveyi by determining their expression levels, both in vitro and in vivo during infection of gnotobiotic brine shrimp. The quorum sensing master regulator luxR and the vhp metalloprotease showed around threefold and fivefold higher ...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2011.00268.x
更新日期:2011-10-01 00:00:00
abstract::In this study, we report the presence of the El Tor CTXΦ and classical CTXΦ in Vibrio cholerae O1 strains isolated from Varanasi, India. Polymerase chain reaction, DNA sequencing and restriction fragment length polymorphism revealed that, although ctx-positive strains isolated after 1990 contain CTXΦ harbouring El Tor...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2011.00287.x
更新日期:2011-12-01 00:00:00
abstract::Previous studies examining the clonal diversity of Escherichia coli populations within humans have been based on faecal isolates. In this study E. coli were isolated from biopsies taken from the terminal ileum, ascending, transverse and descending colon, and rectum of 69 individuals. Multiple isolates from each biopsy...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12300
更新日期:2015-08-01 00:00:00
abstract::Rice plants play a key role in regulating methane emissions from paddy fields by affecting both underlying processes: methane production and oxidation. Specific differences were reported for methane oxidation rates; however, studies on the bacterial communities involved are rare. Here, we analysed the methanotrophic c...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2011.00251.x
更新日期:2011-10-01 00:00:00
abstract::The bacterial adenylate cyclase two-hybrid (BACTH) system has been widely used to characterize protein-protein interactions in the prokaryotic world. This system relies on the interaction-mediated reconstitution of adenylate cyclase activity in Escherichia coli by bringing together two complementary fragments of the c...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12123
更新日期:2014-06-01 00:00:00
abstract::Bacterioplankton play a critical role in primary production, carbon cycling, and nutrient cycling in the oligotrophic ocean. To investigate the effect of elevated CO2 and warming on the composition and function of bacterioplankton communities in oligotrophic waters, we performed two trace-metal clean deck board incuba...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12844
更新日期:2020-08-01 00:00:00
abstract::In anoxic environments such as swamps, rice fields and sludge digestors, syntrophic microbial communities are important for decomposition of organic matter to CO2 and CH4 . The most difficult step is the fermentative degradation of short-chain fatty acids such as propionate and butyrate. Conversion of these metabolite...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/j.1758-2229.2010.00147.x
更新日期:2010-08-01 00:00:00
abstract::The trophic interactions between viruses, bacteria and protists play a crucial role in structuring microbial communities and regulating nutrient and organic matter flux. Here, we show that the impact on viral density by heterotrophic flagellates is related to their feeding behaviour (feeding on sedimented particles - ...
journal_title:Environmental microbiology reports
pub_type: 杂志文章
doi:10.1111/1758-2229.12119
更新日期:2014-08-01 00:00:00