当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Roles of different pools of the mitotic checkpoint complex and the mechanisms of their disassembly.

Roles of different pools of the mitotic checkpoint complex and the mechanisms of their disassembly.

Abstract：

:The mitotic (or spindle assembly) checkpoint system prevents premature separation of sister chromatids in mitosis. When the checkpoint is turned on, the mitotic checkpoint complex (MCC) inhibits the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C). MCC is composed of the checkpoint proteins BubR1, Bub3, and Mad2 associated with the APC/C activator Cdc20. The mechanisms of the assembly of MCC when the checkpoint is turned on, and of its disassembly when the checkpoint is inactivated, are not sufficiently understood. Previous reports indicated that APC/C-mediated polyubiquitylation of Cdc20 in MCC is required for the dissociation of APC/C-associated MCC, but not of free MCC. The pool of free MCC is disassembled by an ATP-dependent process stimulated by the Mad2-binding protein p31(comet). It remained unknown whether free MCC is the precursor or the dissociation product of APC/C-bound MCC. By characterizing the mechanisms of the disassembly of APC/C-bound MCC in a purified system, we find that it cannot be the source of free MCC, because it is bound at high affinity and is released only in ubiquitylated or partially disassembled forms. By the use of a cell-free system from Xenopus eggs that reproduces the mitotic checkpoint, we show that MCC can be assembled in the absence of APC/C in a checkpoint-dependent manner. We propose that when the checkpoint is turned on, free MCC is the precursor of APC/C-bound MCC. When the mitotic checkpoint is extinguished, both APC/C-bound and free MCC pools have to be disassembled to release APC/C from inhibition.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Eytan E,Sitry-Shevah D,Teichner A,Hershko A

doi

10.1073/pnas.1308928110

subject

Has Abstract

pub_date

2013-06-25 00:00:00

pages

10568-73

issue

26

eissn

0027-8424

issn

1091-6490

pii

1308928110

journal_volume

110

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Dual regulation of NR2B and NR2C expression by NMDA receptor activation in mouse cerebellar granule cell cultures.

    abstract：:In the developing cerebellum, switching of the subunit composition of NMDA receptors occurs in granule cells from NR2B-containing receptors to NR2C-containing ones. We investigated the mechanisms underlying switching of NR2B and NR2C subunit composition in primary cultures of mouse granule cells at the physiological K...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0805574105

    authors： Iijima K,Abe H,Okazawa M,Moriyoshi K,Nakanishi S

    更新日期：2008-08-19 00:00:00

  • Jagged-mediated Notch signaling maintains proliferating neural progenitors and regulates cell diversity in the ventral spinal cord.

    abstract：:Previous studies have shown that Delta-mediated Notch signaling regulates the number of early differentiating neurons. However, the role of Notch activation and Jagged-mediated signaling during late neurogenesis remains poorly defined. In the developing spinal cord of zebrafish, GABAergic Kolmer-Agduhr (KA'') cells an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0607062104

    authors： Yeo SY,Chitnis AB

    更新日期：2007-04-03 00:00:00

  • Identification of genes that synergize with Cbfb-MYH11 in the pathogenesis of acute myeloid leukemia.

    abstract：:Acute myeloid leukemia subtype M4 with eosinophilia is associated with a chromosome 16 inversion that creates a fusion gene CBFB-MYH11. We have previously shown that CBFB-MYH11 is necessary but not sufficient for leukemogenesis. Here, we report the identification of genes that specifically cooperate with CBFB-MYH11 in...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0400930101

    authors： Castilla LH,Perrat P,Martinez NJ,Landrette SF,Keys R,Oikemus S,Flanegan J,Heilman S,Garrett L,Dutra A,Anderson S,Pihan GA,Wolff L,Liu PP

    更新日期：2004-04-06 00:00:00

  • Ras activity regulates cyclin E degradation by the Fbw7 pathway.

    abstract：:The Skp1-Cullin1 F-box protein-Fbw7 ubiquitin ligase regulates phosphorylation-dependent cyclin E degradation, and disruption of this pathway is associated with genetic instability and tumorigenesis. Fbw7 is a human tumor suppressor that is targeted for mutation in primary cancers. However, mechanisms other than mutat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0503677102

    authors： Minella AC,Welcker M,Clurman BE

    更新日期：2005-07-05 00:00:00

  • Neutrinos.

    abstract：:Neutrinos represent a new "window" to the Universe, spanning a large range of energy. We discuss the science of neutrino astrophysics and focus on two energy regimes. At "lower" energies ( approximately 1 MeV), studies of neutrinos born inside the sun, or produced in interactions of cosmic rays with the atmosphere, ha...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.25.14201

    authors： Besson D,Cowen D,Selen M,Wiebusch C

    更新日期：1999-12-07 00:00:00

  • Design and construction of diverse mammalian prion strains.

    abstract：:Prions are infectious proteins that encipher biological information within their conformations; variations in these conformations dictate different prion strains. Toward elucidating the molecular language of prion protein (PrP) conformations, we produced an array of recombinant PrP amyloids with varying conformational...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0910350106

    authors： Colby DW,Giles K,Legname G,Wille H,Baskakov IV,DeArmond SJ,Prusiner SB

    更新日期：2009-12-01 00:00:00

  • Uridine diphosphate N-acetylglucosamine stimulates uptake of nutrients by quiescent BALB/C3T3 cells.

    abstract：:Preincubation of quiescent BALB/C3T3 cells with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) in conditioned medium resulted in a time- and concentration-dependent stimulation of uptake of 2-deoxyglucose, uridine, and alpha-aminoisobutyric acid over that seen in conditioned medium alone. The apparent Km values ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.8.3859

    authors： Natraj CV,Datta P

    更新日期：1978-08-01 00:00:00

  • Conformational states and recognition of amyloidogenic peptides of human insulin-degrading enzyme.

    abstract：:Insulin-degrading enzyme (IDE) selectively degrades the monomer of amyloidogenic peptides and contributes to clearance of amyloid β (Aβ). Thus, IDE retards the progression of Alzheimer's disease. IDE possesses an enclosed catalytic chamber that engulfs and degrades its peptide substrates; however, the molecular mechan...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1304575110

    authors： McCord LA,Liang WG,Dowdell E,Kalas V,Hoey RJ,Koide A,Koide S,Tang WJ

    更新日期：2013-08-20 00:00:00

  • Patterning cells and their environments using multiple laminar fluid flows in capillary networks.

    abstract：:This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cell...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.10.5545

    authors： Takayama S,McDonald JC,Ostuni E,Liang MN,Kenis PJ,Ismagilov RF,Whitesides GM

    更新日期：1999-05-11 00:00:00

  • Site-specific gene delivery in vivo through engineered Sendai viral envelopes.

    abstract：:Inspite of several stimulating developments in gene therapy, the formulation of a targeted gene delivery "vector" is still far from ideal. We have demonstrated the potential of reconstituted Sendai viral envelopes containing only the fusion glycoprotein (F-virosomes) in targeted delivery of reporter genes to liver cel...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.20.11886

    authors： Ramani K,Hassan Q,Venkaiah B,Hasnain SE,Sarkar DP

    更新日期：1998-09-29 00:00:00

  • Ribosomal RNA genes of Neurospora: isolation and characterization.

    abstract：:DNA sequences in Neurospora crassa that code for ribosomal RNA have been isolated in a highly purified state by DNA-RNA hybridization. About 100 separate ribosomal RNA genes exist in each nucleus. These genes are composed of repeated sequences that are present about 100 times per nucleus. The individual members of thi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.11.3256

    authors： Chattopadhyay SK,Kohne DE,Dutta SK

    更新日期：1972-11-01 00:00:00

  • Synthesis of complementary RNA sequences during productive adenovirus infection.

    abstract：:Liquid RNA.DNA hybridization with separated strands of adenovirus type 2 DNA revealed that late nuclear RNA can hybridize to about 85% of the 1-strand and 10-15% of the h-strand, whereas late cytoplasmic RNA hybridizes to 65-70% and 25% of the l- and h-strand, respectively. With separated strands from the six EcoRI fr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.12.4887

    authors： Pettersson U,Philipson L

    更新日期：1974-12-01 00:00:00

  • Error rates for nanopore discrimination among cytosine, methylcytosine, and hydroxymethylcytosine along individual DNA strands.

    abstract：:Cytosine, 5-methylcytosine, and 5-hydroxymethylcytosine were identified during translocation of single DNA template strands through a modified Mycobacterium smegmatis porin A (M2MspA) nanopore under control of phi29 DNA polymerase. This identification was based on three consecutive ionic current states that correspond...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1310615110

    authors： Schreiber J,Wescoe ZL,Abu-Shumays R,Vivian JT,Baatar B,Karplus K,Akeson M

    更新日期：2013-11-19 00:00:00

  • Alkane biosynthesis by decarbonylation of aldehydes catalyzed by a particulate preparation from Pisum sativum.

    abstract：:Mechanism of enzymatic conversion of a fatty acid to the corresponding alkane by the loss of the carboxyl carbon was investigated with particulate preparations from Pisum sativum. A heavy particulate preparation (sp. gr., 1.30 g/cm3) isolated by two density-gradient centrifugation steps catalyzed conversion of octadec...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.21.6613

    authors： Cheesbrough TM,Kolattukudy PE

    更新日期：1984-11-01 00:00:00

  • Genetic mapping of Rhizobium meliloti.

    abstract：:The drug resistance factor RP4, originally isolated in Pseudomonas, was transferred to Rhizobium meliloti. In that strain, RP4 promotes conjugational transfer of chromosomal markers to form haploid recombinants. This mating system has been used to construct a linkage map of R. meliloti. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.5.2076

    authors： Meade HM,Signer ER

    更新日期：1977-05-01 00:00:00

  • Niches, models, and climate change: assessing the assumptions and uncertainties.

    abstract：:As the rate and magnitude of climate change accelerate, understanding the consequences becomes increasingly important. Species distribution models (SDMs) based on current ecological niche constraints are used to project future species distributions. These models contain assumptions that add to the uncertainty in model...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,评审

    doi：10.1073/pnas.0901639106

    authors： Wiens JA,Stralberg D,Jongsomjit D,Howell CA,Snyder MA

    更新日期：2009-11-17 00:00:00

  • Role for ERK1/2-dependent activation of FCHSD2 in cancer cell-selective regulation of clathrin-mediated endocytosis.

    abstract：:Clathrin-mediated endocytosis (CME) regulates the uptake of cell-surface receptors as well as their downstream signaling activities. We recently reported that signaling can reciprocally regulate CME in cancer cells and that this crosstalk can contribute to cancer progression. To further explore the nature and extent o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1810209115

    authors： Xiao GY,Mohanakrishnan A,Schmid SL

    更新日期：2018-10-09 00:00:00

  • Evidence that DNA is present in abnormal tubulofilamentous structures found in scrapie.

    abstract：:Abnormal tubulofilamentous structures have been identified in electron micrographs of thin sections and negatively stained impression grids prepared from brains of animals with scrapie and other spongiform encephalopathies, and we showed that such tubules contain a core of filamentous structures resembling scrapie-ass...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.10.3575

    authors： Narang HK,Asher DM,Gajdusek DC

    更新日期：1988-05-01 00:00:00

  • The pentameric complex drives immunologically covert cell-cell transmission of wild-type human cytomegalovirus.

    abstract：:Human cytomegalovirus (HCMV) strains that have been passaged in vitro rapidly acquire mutations that impact viral growth. These laboratory-adapted strains of HCMV generally exhibit restricted tropism, produce high levels of cell-free virus, and develop susceptibility to natural killer cells. To permit experimentation ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1704809114

    authors： Murrell I,Bedford C,Ladell K,Miners KL,Price DA,Tomasec P,Wilkinson GWG,Stanton RJ

    更新日期：2017-06-06 00:00:00

  • The colorectal microRNAome.

    abstract：:MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we hav...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0511155103

    authors： Cummins JM,He Y,Leary RJ,Pagliarini R,Diaz LA Jr,Sjoblom T,Barad O,Bentwich Z,Szafranska AE,Labourier E,Raymond CK,Roberts BS,Juhl H,Kinzler KW,Vogelstein B,Velculescu VE

    更新日期：2006-03-07 00:00:00

  • Reduction of ischemia and reperfusion-induced myocardial damage by cytochrome P450 inhibitors.

    abstract：:Ischemia and reperfusion both contribute to tissue damage after myocardial infarction. Although many drugs have been shown to reduce infarct size when administered before ischemia, few have been shown to be effective when administered at reperfusion. Moreover, although it is generally accepted that a burst of reactive...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0308185100

    authors： Granville DJ,Tashakkor B,Takeuchi C,Gustafsson AB,Huang C,Sayen MR,Wentworth P Jr,Yeager M,Gottlieb RA

    更新日期：2004-02-03 00:00:00

  • Indirect reciprocity with private, noisy, and incomplete information.

    abstract：:Indirect reciprocity is a mechanism for cooperation based on shared moral systems and individual reputations. It assumes that members of a community routinely observe and assess each other and that they use this information to decide who is good or bad, and who deserves cooperation. When information is transmitted pub...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1810565115

    authors： Hilbe C,Schmid L,Tkadlec J,Chatterjee K,Nowak MA

    更新日期：2018-11-27 00:00:00

  • Differences in quantal amplitude reflect GluR4- subunit number at corticothalamic synapses on two populations of thalamic neurons.

    abstract：:Low-frequency thalamocortical oscillations that underlie drowsiness and slow-wave sleep depend on rhythmic inhibition of relay cells by neurons in the reticular nucleus (RTN) under the influence of corticothalamic fibers that branch to innervate RTN neurons and relay neurons. To generate oscillations, input to RTN pre...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.061013698

    authors： Golshani P,Liu XB,Jones EG

    更新日期：2001-03-27 00:00:00

  • Natal dispersal driven by environmental conditions interacting across the annual cycle of a migratory songbird.

    abstract：:Natal dispersal, the process through which immature individuals permanently depart their natal area in search of new sites, is integral to the ecology and evolution of animals. Insights about the underlying causes of natal dispersal arise mainly from research on species whose short dispersal distances or restricted di...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0710732105

    authors： Studds CE,Kyser TK,Marra PP

    更新日期：2008-02-26 00:00:00

  • Retroposon analysis and recent geological data suggest near-simultaneous divergence of the three superorders of mammals.

    abstract：:As a consequence of recent developments in molecular phylogenomics, all extant orders of placental mammals have been grouped into 3 lineages: Afrotheria, Xenarthra, and Boreotheria, which originated in Africa, South America, and Laurasia, respectively. Despite this advancement, the order of divergence of these 3 linea...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0809297106

    authors： Nishihara H,Maruyama S,Okada N

    更新日期：2009-03-31 00:00:00

  • Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus.

    abstract：:Resistance to cobalt, zinc, and cadmium specified by the czc determinant on plasmid pMOL30 in Alcaligenes eutrophus results from a cation efflux system. Five membrane-bound polypeptides that were expressed in Escherichia coli from this determinant under the control of a phage T7 promoter were assigned to four open rea...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.19.7351

    authors： Nies DH,Nies A,Chu L,Silver S

    更新日期：1989-10-01 00:00:00

  • Evidence for a chimeric nature of nuclear genomes: eubacterial origin of eukaryotic glyceraldehyde-3-phosphate dehydrogenase genes.

    abstract：:Higher plants process two distinct, nuclear gene-encoded glyceraldehyde-3-phosphate dehydrogenase (GAPDH) proteins, a Calvin-cycle enzyme active within chloroplasts and a glycolytic enzyme active within the cytosol. The gene for the chloroplast enzyme was previously suggested to be of endosymbiotic origin. Since the a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.18.8692

    authors： Martin W,Brinkmann H,Savonna C,Cerff R

    更新日期：1993-09-15 00:00:00

  • Anisotropic particles align perpendicular to the flow direction in narrow microchannels.

    abstract：:The flow orientation of anisotropic particles through narrow channels is of importance in many fields, ranging from the spinning and molding of fibers to the flow of cells and proteins through thin capillaries. It is commonly assumed that anisotropic particles align parallel to the flow direction. When flowing through...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1219340110

    authors： Trebbin M,Steinhauser D,Perlich J,Buffet A,Roth SV,Zimmermann W,Thiele J,Förster S

    更新日期：2013-04-23 00:00:00

  • The primary structure of sensory rhodopsin II: a member of an additional retinal protein subgroup is coexpressed with its transducer, the halobacterial transducer of rhodopsin II.

    abstract：:The blue-light receptor genes (sopII) of sensory rhodopsin (SR) II were cloned from two species, the halophilic bacteria Haloarcula vallismortis (vSR-II) and Natronobacterium pharaonis (pSR-II). Upstream of both sopII gene loci, sequences corresponding to the halobacterial transducer of rhodopsin (Htr) II were recogni...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.7.3036

    authors： Seidel R,Scharf B,Gautel M,Kleine K,Oesterhelt D,Engelhard M

    更新日期：1995-03-28 00:00:00

  • Oligomerization of opioid receptors with beta 2-adrenergic receptors: a role in trafficking and mitogen-activated protein kinase activation.

    abstract：:G-protein-coupled receptors (GPCRs) have recently joined the list of cell surface receptors that dimerize. Dimerization has been shown to alter the ligand-binding, signaling, and trafficking properties of these receptors. Recent studies have shown that GPCRs heterodimerize with closely related members, resulting in th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.011384898

    authors： Jordan BA,Trapaidze N,Gomes I,Nivarthi R,Devi LA

    更新日期：2001-01-02 00:00:00