Abstract:
INTRODUCTION:Mesenchymal stem cells (MSCs) are a promising tool for regenerative medicine, but due to the heterogeneity of their populations, different sources and isolation techniques, the characteristics defining MSCs are inconsistent. OBJECTIVE:The aim of this study was to compare the characteristics of MSCs derived from two different human tissues: peripheral blood (PB-MSCs) and umbilical cord Wharton's Jelly (UC-MSCs). METHODS:The PB-MSC and UC-MSC were isolated by adherence to plastic after gradient-density separation or an explant culture method, respectively, and compared regarding their morphology, clonogenic efficiency, proliferating rates, immunophenotype and differentiation potential. RESULTS:MSCs derived from both sources exhibit similar morphology, proliferation capacity and multilineage (osteogenic, chondrogenic, adipogenic and myogenic) differentiation potential. Differences were observed in the clonogenic capacity and the immunophenotype, since UC-MSCs showed higher CFU-F (colony-forming units-fibroblastic) cloning efficiency, as well as higher embryonic markers (Na-nog, Sox2, SSEA4) expression. When additional surface antigens were analyzed by flow cytometry (CD44, CD90, CD105, CD33, CD34, CD45, CD11b, CD235a) or immunofluorescent labeling (vimentin, STRO-1 and alpha-smooth muscle actin), most appeared to have similar epitope profiles irrespective of MSC source. CONCLUSION:The results obtained demonstrated that both MSCs represent good alternative sources of adult MSCs that could be used in cell therapy applications.
journal_name
Srp Arh Celok Lekjournal_title
Srpski arhiv za celokupno lekarstvoauthors
Trivanović D,Kocić J,Mojsilović S,Krstić A,Ilić V,Djordjević IO,Santibanez JF,Jovcić G,Terzić M,Bugarski Ddoi
10.2298/sarh1304178tsubject
Has Abstractpub_date
2013-03-01 00:00:00pages
178-86issue
3-4eissn
0370-8179issn
2406-0895journal_volume
141pub_type
杂志文章abstract:INTRODUCTION:Uretero-intestinal anastomisis has a large influence on derivation quality. OBJECTIVE:The aim of the study was to present the modified serous lined extramural Abol-Enein method of implantation of low quality ureter into the sigma-rectum pouch and comparison of the results with Le Duc implantation. METHOD...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1108491s
更新日期:2011-07-01 00:00:00
abstract::The first written serbian medical records could be found in the Middle Ages (XIIIth century) when the serbian state was very well organized. St. Save was the son of Grand-Prince Stefan Nemanja (1171-1196), founder of the Serbian independent state and of a powerful dynasty that ruled Serbia for two centuries. At that t...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 传,历史文章,杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::The paper is aimed at illustrating the importance of nonspecific and specific bronchoprovokative tests for diagnosing allergic bronchial asthma by pathophysiologic mechanisms of hyperiritability of airways and making of specific "schok tissue". Besides we tried to explain these exeptional cases which we encounter in o...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::The recent data about the treatment of advanced breast cancer patients with synthetic LH-RH analogues are presented. Although synthetized 20 years ago, the agonistic analogues of LH-RH were introduced in clinical oncology recently. The inhibition of pituitary and gonadal function that occurs after chronic administrati...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract:INTRODUCTION:Compliance with hypertension guidelines in clinical practice is generally poor, but there was no data about compliance with any guidelines in general practice in Slovenia. OBJECTIVE:Our aim was to find out whether general practitioners in Slovenia managed their hypertensive patients according to the natio...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh0704191p
更新日期:2007-03-01 00:00:00
abstract::This paper is a continuing evaluation of dental and facial parameters in the estimation of aesthetic characteristic of fixed restorations. First of all, attention is paid to the phenomenon describing the tooth tissue's characteristics (translucency, opalescence, and transparency). The paper also discusses tooth colour...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章,评审
doi:10.2298/sarh0506288o
更新日期:2005-05-01 00:00:00
abstract::From a historic point of view, epilepsy and its eponyms were in an ontogenetic symbiosis throughout their history. Epilepsy is a disease with a history of eponyms presenting the frame of mind of both streetwise as well as skilled "authors" about its origin and nature. From ancient times the names for epilepsy, archety...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 历史文章,杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::In the last two decades the clinical and research focus in psychotic disorders has shifted to first episode psychosis, early detection of the prodromal phase of psychosis and an effective integrated treatment model known as "Early Intervention". The first five years of psychosis that is described as a "critical period...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章,评审
doi:
更新日期:2007-11-01 00:00:00
abstract::This is a case report of 26-year old, advanced university student of the Faculty of Science, Department of Mathematics, single. He has never been a psychiatric patient, but being unable to suppress the gambling impulse, he presented for treatment, on his own initiative, to the outpatient department. Patient met the cr...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh0602060m
更新日期:2006-01-01 00:00:00
abstract::In three patients (mean age about 3 years) we established excretory tract duplication with obstructed ectopic ureter, a function of the upper pole and ectopic ureterocele. Diagnostic procedure was different in each case. Ultrasonography was the most important diagnostic method in all cases; it usually showed all main ...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract:INTRODUCTION:Term breech presentation occurs in 3-4% of all deliveries. Most obstetricians are completely sure how to end delivery when the foetus is in breech presentation, by caesarean section as the only option. OBJECTIVE:The main goal of the paper was to present the method of delivery for term breech presentation ...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1104155d
更新日期:2011-03-01 00:00:00
abstract::The frequency and the degree of bronchial hyperreactivity was investigated in 92 patients suffering from chronic obstructive bronchitis. Bronchial reactivity was examgned by the inhalator acetylcholine test, with the use of a 5 mg dose of acetylcholine, diluted in 1 ml of sodium-chloride solution. The pulmonary functi...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract:INTRODUCTION:Reduced compound glutathione (GSH) in the lens has the function to protect the thiol group of lens proteins, and as a substrate of glutathione peroxidase (GPx) and glutathione S-transferase (GST). Protein containing thiol groups is significant for the normal function of lens epithelium, i.e. enzymes Na-K-A...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1210563k
更新日期:2012-09-01 00:00:00
abstract::A patient, aged 28, was admitted to the Department of Urology of the Military Medical Academy in Belgrade, for the operation of the right adrenal gland tumour. The adrenal gland tumour was first diagnosed as an inactive hormonal tumour. Intraoperatively it was found in retroperitoneal area, over the right adrenal glan...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract:INTRODUCTION:Paget disease, localized on thoracal vertebrae, presents a significant challenge in diagnostics and treatment. Presenting with progressive neurological symptoms, it has a broad differential diagnostic spectrum and requires additio-nal radiological, neurological and endocrinological evaluation. Besides drug...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh0808419v
更新日期:2008-07-01 00:00:00
abstract:INTRODUCTION:Perthes disease is idiopathic avascular osteonecrosis of the hip in children, with unknown etiology. Inflammation is present during development of Perthes disease and it is known that this process influences bone remodeling. OBJECTIVE:Since genetic studies related to inflammation have not been performed i...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract:INTRODUCTION:Cesarean delivery is the most common obstetric surgery today. Improvement of operative techniques, anaesthesia, care and transfusion has contributed to the safety of the procedure and expansion of indications. The operative technique usually consists of opening the abdominal wall by inferior transverse inc...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章,随机对照试验
doi:10.2298/sarh08s2109v
更新日期:2008-05-01 00:00:00
abstract::Our results of surgery of developmental disorder of the hip (DDH) in older children and adolescents by using the combination of Selter's innominate osteotomy and osteotomy of the superior pubis ramus were presented in this paper, enclosing the period from 1993 to 1995. The study included 11 hips, i.e. 9 patients, aged...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh04s1023b
更新日期:2004-10-01 00:00:00
abstract:INTRODUCTION:Diseases of the gastrointestinal system, and especially diseases of esophagus, are a rare cause of sudden unexpected death in any age group. Black esophagus is an acute necrotizing inflammation of the mucosa of the lower parts of esophagus, diffusely affecting its entire circumference. This inflammation co...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1402094n
更新日期:2014-01-01 00:00:00
abstract::The ileal pouch-anal operation is the best procedure because it liberates the patient from ulcerative colitis i.e. from diseased mucosa of the large intestine. The ileal pouch forms a new reservoir for the storage of feces. Symptomatic pouch inflammation (pouchitis) appeared in 7-40% of the patients. The authors descr...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::Bladder cancer is a common form of neoplasia which most often presents histologically as urothelial (transitional cell) carcinoma. In this article we review recent publications dealing with the less common variants of urothelial carcinoma such as tumours that show unusual forms of differentiation or the well know squa...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章,评审
doi:
更新日期:2011-09-01 00:00:00
abstract:INTRODUCTION:Penetrated injuries are most difficult injuries of the eye. Intraocular foreign body (IOFB) may lodge in any of the structures it encounters, from anterior chamber to the retina and choroid. Notable effects caused by foreign body injury include traumatic cataract, vitreous liquefaction, retinal and subreti...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:2013-07-01 00:00:00
abstract:INTRODUCTION:The seroprevalence study was performed in Vojvodina during May and June 2010 in order to asses the effects of the 2009 pandemic influenza A(H1N1)v epidemic on herd immunity. It was a part of the Serbian Ministry of Health funded nationwide study. OBJECTIVE:Prevalence of antibodies against 2009 pandemic in...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:2012-11-01 00:00:00
abstract:INTRODUCTION:The development of effective medications makes pharmacological therapy of BPH the dominant mode of treatment today. It improves urinary symptoms and prevents disease progression while producing side effects on male sexual function. OBJECTIVE:The aim of the study is to present the effects of BPH pharmacolo...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1506284s
更新日期:2015-05-01 00:00:00
abstract:INTRODUCTION:The paper presents important facts in the application of recombinant human factor VIIa (rFVIIa) and in the treatment of renal cell carcer. CASE REPORT:A 69-year old male with infiltrative renal cell carcer underwent radical nephrectomy using Hasagawa's approach. The extirpated tumor was 35 cm in diameter....
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh08s3259j
更新日期:2008-09-01 00:00:00
abstract:INTRODUCTION:The causes of acute tubulointerstitial nephritis can be grouped into four broad categories: medications, infections, immunologic diseases, or idiopathic processes. Here we report a 17-year-old female who developed acute kidney injury (AKI) due to granulomatous interstitial nephritis (GIN) associated with i...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:
更新日期:2016-03-01 00:00:00
abstract:INTRODUCTION:Endovascular aneurysm repair (EVAR) has been introduced into clinical practice at the beginning of the 90's of the last century. Because of economic, political and social problems during the last 25 years, the introduction of this procedure in Serbia was not possible. OBJECTIVE:The aim of this study was t...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh0902010d
更新日期:2009-01-01 00:00:00
abstract::During laryngomicroscopy, the superficial layers of vocal fold epithelium can be examined in vivo and in situ by contact endoscopy. Methylene blue is applied initially to stain the epihelial cells of the vocal folds. When in contact with mucosal tissue, this endoscope provides 60 and 150 times magnification and clear ...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh0504134j
更新日期:2005-03-01 00:00:00
abstract::The clinical end-point of all causes of mortality and cardiovascular hospitalisation (combined end-points) is a widely accepted indicator of heart failure survival. The primary aim of this study was to examine the effects of metoprolol and atenolol on combined end-points in patients with mild-to-moderate heart failure...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章,随机对照试验
doi:10.2298/sarh0506242c
更新日期:2005-05-01 00:00:00
abstract:INTRODUCTION:Although the third most common aneurysm within the abdomen, after aneurysms of the aorta and iliac arteries, splenic artery aneurysms are rare, but not exceptionally. Owing to new imaging techniques, they have been discovered with increasing frequency. CASE OUTLINE:Authors present a 47-year-old woman, mul...
journal_title:Srpski arhiv za celokupno lekarstvo
pub_type: 杂志文章
doi:10.2298/sarh1012760c
更新日期:2010-11-01 00:00:00