Abstract:
:The present study was undertaken to characterize different vitellogenins in Channa punctatus. Protein purification by gel chromatography followed by fast protein liquid chromatography (FPLC) revealed existence of two different Vg forms. Liquid chromatography tandem mass spectrophotometry (LC-MS/MS) suggested the existence of Vga and Vgb. Cloning of partial sequences of vga and vgb mRNA and phylogenetic analysis substantiated the existence of two vitellogenins. Real time PCR for vga and vgb genes from liver of estradiol-17β (E2) treated fish reveals difference in expression levels of transcripts of these two genes. vgb is expressed at lower dose of estradiol suggesting a higher sensitivity to estradiol. The present study thus proposes different regulatory control for the expression of these two genes and vgb as a superior biomarker than vga to assess exposure of C. punctatus to environmental estrogens.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Rawat VS,Pipil S,Sharma L,Sehgal Ndoi
10.1016/j.ygcen.2013.05.002subject
Has Abstractpub_date
2013-08-01 00:00:00pages
119-26eissn
0016-6480issn
1095-6840pii
S0016-6480(13)00216-5journal_volume
189pub_type
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journal_title:General and comparative endocrinology
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journal_title:General and comparative endocrinology
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journal_title:General and comparative endocrinology
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journal_title:General and comparative endocrinology
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