Abstract:
:Methane is a potent greenhouse gas that is produced in significant quantities by aerobic marine organisms. These bacteria apparently catalyse the formation of methane through the cleavage of the highly unreactive carbon-phosphorus bond in methyl phosphonate (MPn), but the biological or terrestrial source of this compound is unclear. However, the ocean-dwelling bacterium Nitrosopumilus maritimus catalyses the biosynthesis of MPn from 2-hydroxyethyl phosphonate and the bacterial C-P lyase complex is known to convert MPn to methane. In addition to MPn, the bacterial C-P lyase complex catalyses C-P bond cleavage of many alkyl phosphonates when the environmental concentration of phosphate is low. PhnJ from the C-P lyase complex catalyses an unprecedented C-P bond cleavage reaction of ribose-1-phosphonate-5-phosphate to methane and ribose-1,2-cyclic-phosphate-5-phosphate. This reaction requires a redox-active [4Fe-4S]-cluster and S-adenosyl-L-methionine, which is reductively cleaved to L-methionine and 5'-deoxyadenosine. Here we show that PhnJ is a novel radical S-adenosyl-L-methionine enzyme that catalyses C-P bond cleavage through the initial formation of a 5'-deoxyadenosyl radical and two protein-based radicals localized at Gly 32 and Cys 272. During this transformation, the pro-R hydrogen from Gly 32 is transferred to the 5'-deoxyadenosyl radical to form 5'-deoxyadenosine and the pro-S hydrogen is transferred to the radical intermediate that ultimately generates methane. A comprehensive reaction mechanism is proposed for cleavage of the C-P bond by the C-P lyase complex that uses a covalent thiophosphate intermediate for methane and phosphate formation.
journal_name
Naturejournal_title
Natureauthors
Kamat SS,Williams HJ,Dangott LJ,Chakrabarti M,Raushel FMdoi
10.1038/nature12061subject
Has Abstractpub_date
2013-05-02 00:00:00pages
132-6issue
7447eissn
0028-0836issn
1476-4687pii
nature12061journal_volume
497pub_type
杂志文章相关文献
NATURE文献大全abstract::Owing to their scientific and technological importance, inorganic single crystals with highly reactive surfaces have long been studied. Unfortunately, surfaces with high reactivity usually diminish rapidly during the crystal growth process as a result of the minimization of surface energy. A typical example is titaniu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature06964
更新日期:2008-05-29 00:00:00
abstract::Synaptic plasticity dependent on N-methyl-D-aspartate (NMDA) receptors is thought to underlie certain types of learning and memory. In support of this, both hippocampal long-term potentiation and spatial learning in a watermaze are impaired by blocking NMDA receptors with a selective antagonist D(-)-2-amino-5-phosphon...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/378182a0
更新日期:1995-11-09 00:00:00
abstract::Four incorrect figure citations in this Letter have been corrected online. ...
journal_title:Nature
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41586-018-0560-x
更新日期:2018-11-01 00:00:00
abstract::The M13 coat protein spans the Escherichia coli plasma membrane with its amino-terminus facing the periplasm. It is made as a precursor--the procoat--with a typical leader peptide. Mutations which destroy the basic character of the carboxy-terminal domain of procoat, a domain which is oriented towards the cytoplasm, b...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/322335a0
更新日期:1986-07-24 00:00:00
abstract::The idea that natural selection on genes might be detected using only a single genome has been put forward by Plotkin and colleagues, who present a method that they claim can detect selection without the need for comparative data and which, if correct, would confer greater power of analysis with less information. Here...
journal_title:Nature
pub_type: 评论,杂志文章
doi:10.1038/nature03221
更新日期:2005-01-20 00:00:00
abstract::On 12 May 2008, the devastating magnitude 7.9 (Wenchuan) earthquake struck the eastern edge of the Tibetan plateau, collapsing buildings and killing thousands in major cities aligned along the western Sichuan basin in China. After such a large-magnitude earthquake, rearrangement of stresses in the crust commonly leads...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature07177
更新日期:2008-07-24 00:00:00
abstract::The cloning of mammals from differentiated donor cells has refuted the old dogma that development is an irreversible process. It has demonstrated that the oocyte can reprogramme an adult nucleus into an embryonic state that can direct development of a new organism. The prospect of deriving patient-specific embryonic s...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/nature04955
更新日期:2006-06-29 00:00:00
abstract::The recent discovery of a pterosaur egg with embryonic skeleton and soft tissues from the Yixian Formation confirmed that the flying pterosaurs were oviparous. Here we describe another pterosaur egg whose exquisite preservation indicates that the shell structure was soft and leathery. ...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/432572a
更新日期:2004-12-02 00:00:00
abstract::It is generally accepted that the oscillatory beating characteristic of sperm flagella is the result of an ATP-induced sliding between the doublet microtubules of the flagellar axoneme, with these longitudinal forces being converted into a lateral bending moment by resistive components of the structure that limit the ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/325351a0
更新日期:1987-01-22 00:00:00
abstract::Chronic electrical stimulation of the auditory nerve in patients with profound sensori-neural deafness is becoming increasingly routine. Therefore, it is important to understand more about the long-term consequences of this procedure. Hitherto, structural studies in animals after electrocochlear stimulation have conce...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/320065a0
更新日期:1986-03-06 00:00:00
abstract::Overfishing of large-bodied benthic fishes and their subsequent population collapses on the Scotian Shelf of Canada's east coast and elsewhere resulted in restructuring of entire food webs now dominated by planktivorous, forage fish species and macroinvertebrates. Despite the imposition of strict management measures i...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10285
更新日期:2011-07-27 00:00:00
abstract::Throughout the latter half of this century, the development and spread of resistance to most front-line antimalarial compounds used in the prevention and treatment of the most severe form of human malaria has given cause for grave clinical concern. Polymorphisms in pfmdr1, the gene encoding the P-glycoprotein homologu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35002615
更新日期:2000-02-24 00:00:00
abstract::COPII-coated vesicles form on the endoplasmic reticulum by the stepwise recruitment of three cytosolic components: Sar1-GTP to initiate coat formation, Sec23/24 heterodimer to select SNARE and cargo molecules, and Sec13/31 to induce coat polymerization and membrane deformation. Crystallographic analysis of the Sacchar...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01040
更新日期:2002-09-19 00:00:00
abstract::A consequence of relativity is that in the presence of an electric field, the spin and momentum states of an electron can be coupled; this is known as spin-orbit coupling. Such an interaction opens a pathway to the manipulation of electron spins within non-magnetic semiconductors, in the absence of applied magnetic fi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02202
更新日期:2004-01-01 00:00:00
abstract::For life to be sustained, mistakes in DNA repair must be tolerated when damage obscures the genetic information. In bacteria such as Escherichia coli, DNA damage elicits the well regulated 'SOS response'. For the extreme case of damage that cannot be repaired by conventional enzymes, there are proteins that allow the ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/380727a0
更新日期:1996-04-25 00:00:00
abstract::When a person walks through a rigid environment while holding eyes and head fixed, the pattern of retinal motion flows radially away from a point, the focus of expansion (Fig. 1a). Under such conditions of translation, heading corresponds to the focus of expansion and people identify it readily. But when making an eye...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/360583a0
更新日期:1992-12-10 00:00:00
abstract::Retinoic acid is a vitamin A derivative with striking effects on development and cell differentiation. Several nuclear retinoic acid receptors (RARs), acting as ligand-inducible transcription factors, have been characterized and indirect evidence suggests that they have distinct roles. One of the most intriguing prope...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/347558a0
更新日期:1990-10-11 00:00:00
abstract::The actions of many peptide growth factors include both stimulation and inhibition of cell proliferation, as well as effects unrelated to the control of cell growth. One peptide can have both stimulatory and inhibitory activity in a single cell, depending on the context of the other signal molecules present. ...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/332217a0
更新日期:1988-03-17 00:00:00
abstract::Vertebrate and invertebrate digestive systems show extensive similarities in their development, cellular makeup and genetic control. The Drosophila midgut is typical: enterocytes make up the majority of the intestinal epithelial monolayer, but are interspersed with hormone-producing enteroendocrine cells. Human (and m...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04333
更新日期:2006-01-26 00:00:00
abstract::Planet formation theories predict that some planets may be ejected from their parent systems as result of dynamical interactions and other processes. Unbound planets can also be formed through gravitational collapse, in a way similar to that in which stars form. A handful of free-floating planetary-mass objects have b...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature23276
更新日期:2017-08-10 00:00:00
abstract::Oncogenic mutations in the serine/threonine kinase B-RAF (also known as BRAF) are found in 50-70% of malignant melanomas. Pre-clinical studies have demonstrated that the B-RAF(V600E) mutation predicts a dependency on the mitogen-activated protein kinase (MAPK) signalling cascade in melanoma-an observation that has bee...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09627
更新日期:2010-12-16 00:00:00
abstract::The current need for novel antibiotics is especially acute for drug-resistant Gram-negative pathogens1,2. These microorganisms have a highly restrictive permeability barrier, which limits the penetration of most compounds3,4. As a result, the last class of antibiotics that acted against Gram-negative bacteria was deve...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1791-1
更新日期:2019-12-01 00:00:00
abstract::Interactions of CD4 with the class II major histocompatibility complex (MHC) are crucial during thymic ontogeny and subsequently for helper and cytotoxic functions of CD4+CD8- T lymphocytes. CD4 is the receptor for the T-lymphotropic human immunodeficiency virus and binds its envelope glycoprotein, gp120. The residues...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/339548a0
更新日期:1989-06-15 00:00:00
abstract::Over half of the inherited predisposition to insulin-dependent diabetes mellitus maps to the region of chromosome 6 that contains the highly polymorphic HLA class II genes which determine immune responsiveness. Analysis of DNA sequences from diabetics indicates that alleles of HLA-DQ beta determine both disease suscep...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/329599a0
更新日期:1987-10-15 00:00:00
abstract::Protein modification by the conjugation of ubiquitin moieties--ubiquitination--plays a major part in many biological processes, including cell cycle and apoptosis. The enzymes that mediate ubiquitin-conjugation have been well-studied, but much less is known about the ubiquitin-specific proteases that mediate de-ubiqui...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01811
更新日期:2003-08-14 00:00:00
abstract::The type I interferon (IFN) response protects cells from viral infection by inducing hundreds of interferon-stimulated genes (ISGs), some of which encode direct antiviral effectors. Recent screening studies have begun to catalogue ISGs with antiviral activity against several RNA and DNA viruses. However, antiviral ISG...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12862
更新日期:2014-01-30 00:00:00
abstract::Most of the progress in human culture has required the exploitation of energy resources. About 100 years ago, the major source of energy shifted from recent solar to fossil hydrocarbons, including liquid and gaseous petroleum. Technology has generally led to a greater use of hydrocarbon fuels for most human activities...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02130
更新日期:2003-11-20 00:00:00
abstract::Thyroglobulin (TG) is the protein precursor of thyroid hormones, which are essential for growth, development and the control of metabolism in vertebrates1,2. Hormone synthesis from TG occurs in the thyroid gland via the iodination and coupling of pairs of tyrosines, and is completed by TG proteolysis3. Tyrosine proxim...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-1995-4
更新日期:2020-02-01 00:00:00
abstract::Molluscs (snails, octopuses, clams and their relatives) have a great disparity of body plans and, among the animals, only arthropods surpass them in species number. This diversity has made Mollusca one of the best-studied groups of animals, yet their evolutionary relationships remain poorly resolved. Open questions ha...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10526
更新日期:2011-10-26 00:00:00
abstract::Spinal cord injury disrupts the communication between the brain and the spinal circuits that orchestrate movement. To bypass the lesion, brain-computer interfaces have directly linked cortical activity to electrical stimulation of muscles, and have thus restored grasping abilities after hand paralysis. Theoretically, ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature20118
更新日期:2016-11-10 00:00:00