Abstract：
:Live-imaging technology has markedly advanced in the field of neural injury and axon degeneration; however, studies are still predominantly performed in in vitro settings such as cultured neuronal cells or in model organisms such as Caenorhabditis elegans in which axons lack glial wrappings. We recently developed a new in vivo model for adult-stage neural injury in Drosophila melanogaster, using the highly accessible wing of the animal. Because the Drosophila wing is translucent and dispensable for survival, it allows clear and direct visualization of injury-induced progressive responses of axons and glia highlighted by fluorescent protein (FP) markers in live animals over time. Moreover, unlike previous Drosophila models of neural injury, this procedure does not require dissection of the CNS. Thus, the key preparation steps for in vivo imaging of the neural injury response described in this protocol can be completed within 30 min.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Fang Y,Soares L,Bonini NMdoi
10.1038/nprot.2013.042subject
Has Abstractpub_date
2013-04-01 00:00:00pages
810-9issue
4eissn
1754-2189issn
1750-2799journal_volume
8pub_type
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