Abstract:
:Vesicular stomatitis virus (VSV) exhibits a remarkably robust and pantropic infectivity, mediated by its coat protein, VSV-G. Using this property, recombinant forms of VSV and VSV-G-pseudotyped viral vectors are being developed for gene therapy, vaccination, and viral oncolysis and are extensively used for gene transduction in vivo and in vitro. The broad tropism of VSV suggests that it enters cells through a highly ubiquitous receptor, whose identity has so far remained elusive. Here we show that the LDL receptor (LDLR) serves as the major entry port of VSV and of VSV-G-pseudotyped lentiviral vectors in human and mouse cells, whereas other LDLR family members serve as alternative receptors. The widespread expression of LDLR family members accounts for the pantropism of VSV and for the broad applicability of VSV-G-pseudotyped viral vectors for gene transduction.
journal_name
Proc Natl Acad Sci U S Aauthors
Finkelshtein D,Werman A,Novick D,Barak S,Rubinstein Mdoi
10.1073/pnas.1214441110subject
Has Abstractpub_date
2013-04-30 00:00:00pages
7306-11issue
18eissn
0027-8424issn
1091-6490pii
1214441110journal_volume
110pub_type
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