Inhibitory effect of skullcap (Scutellaria baicalensis) extract on ovalbumin permeation in vitro and in vivo.

Abstract:

:Scutellaria baicalensis Georgi (skullcap) has been widely used as a dietary ingredient. The purpose of this study was to reveal novel function of skullcap and its mechanism on allergen permeation in intestinal epithelial cells. Intestinal epithelial Caco-2 cell monolayers were used to evaluate the inhibitory effect of skullcap on ovalbumin (OVA) permeation by measuring transepithelial electrical resistance (TEER) and the quantity of permeated OVA. TEER increased and the OVA flux decreased in a dose-dependent manner through up-regulating tight junction-related proteins in cells incubated with increasing concentrations of skullcap extract. In the in vivo study, the amounts of OVA from orally ingested albumen reduced on administration of the skullcap extract. We also revealed for the first time that the active component of skullcap extract for inhibition of OVA permeation was baicalein. These findings demonstrated that skullcap extract might attenuate a food allergic response by inhibiting allergen permeation in vitro and in vivo.

journal_name

Food Chem

journal_title

Food chemistry

authors

Shin HS,Bae MJ,Jung SY,Shon DH

doi

10.1016/j.foodchem.2013.01.042

subject

Has Abstract

pub_date

2013-09-01 00:00:00

pages

22-30

issue

1-2

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(13)00066-6

journal_volume

140

pub_type

杂志文章