Abstract：
:Solving the crystal structure of Cbl(TKB) in complex with a pentapeptide, pYTPEP, revealed that the PEP region adopted a poly-L-proline type II (PPII) helix. An unnatural amino acid termed a proline-templated glutamic acid (ptE) that constrained both the backbone and sidechain to the bound conformation was synthesized and incorporated into the pYTPXP peptide. We estimated imposing structural constraints onto the backbone and sidechain of the peptide and preorganize it to the bound conformation in solution will yield nearly an order of magnitude improvement in activity. NMR studies confirmed that the ptE-containing peptide adopts the PPII conformation, however, competitive binding studies showed an order of magnitude loss of activity. Given the emphasis that is placed on imposing structural constraints, we provide an example to support the contrary. These results point to conformational flexibility at the interface, which have implications in the design of potent Cbl(TKB)-binding peptides.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kumar EA,Chen Q,Kizhake S,Kolar C,Kang M,Chang CE,Borgstahl GE,Natarajan Adoi
10.1038/srep01639subject
Has Abstractpub_date
2013-01-01 00:00:00pages
1639issn
2045-2322pii
srep01639journal_volume
3pub_type
杂志文章abstract：:Attention can be allocated to mental representations to select information from working memory. To date, it remains ambiguous whether such retroactive shifts of attention involve the inhibition of irrelevant information or the prioritization of relevant information. Investigating asymmetries in posterior alpha-band os...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70004-2
更新日期：2020-08-17 00:00:00
abstract：:Fat character is an important index in duck culture that linked to local flavor, feed cost and fat intake for costumers. Since the regulation networks in duck lipid metabolism had not been reported very clearly, we aimed to explore the potential miRNA-mRNA pairs and their regulatory roles in duck lipid metabolism. Her...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27418
更新日期：2016-06-08 00:00:00
abstract：:A novel three dimensional blood brain barrier (BBB) platform was developed by independently supplying different types of media to separate cell types within a single device. One channel (vascular channel, VC) is connected to the inner lumen of the vascular network while the other supplies media to the neural cells (ne...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07416-0
更新日期：2017-08-14 00:00:00
abstract：:Dendritic spines are small, bulbous protrusions along dendrites in neurons and play a critical role in synaptic transmission. Dendritic spines come in a variety of shapes that depend on their developmental state. Additionally, roughly 14-19% of mature spines have a specialized endoplasmic reticulum called the spine ap...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48028-0
更新日期：2019-08-12 00:00:00
abstract：:This paper investigates the possibility of saving a network that is predicted to have a cascading failure that will eventually lead to a total collapse. We model cascading failures using the recently proposed KQ model. Then predict an impending total collapse by monitoring critical slowing down indicators and subseque...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25591-6
更新日期：2018-05-10 00:00:00
abstract：:Hepatocellular carcinoma is the third leading cause of cancer-related deaths worldwide. Many patients are not eligible for curative therapies, such as surgical resection of the tumor or a liver transplant. Transarterial embolization is one therapy clinically used in these cases; however, this requires a long procedure...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47309-y
更新日期：2019-07-30 00:00:00
abstract：:The use of O-(2-[18F]fluoroethyl)-L-tyrosine ([18F]FET) as a positron emission tomography (PET) tracer for brain tumor imaging might have some limitations because of the relatively low affinity for the L-type amino acid transporter 1 (LAT1). To assess the stereospecificity and evaluate the influence of aromatic ring m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40013-x
更新日期：2019-02-27 00:00:00
abstract：:Spin fluctuations are widely believed to play an important role in the superconducting mechanisms of unconventional high temperature superconductors. Spin fluctuations have been observed in iron-based superconductors as well. However, in some iron-based superconductors such as LaFePO0.9, they have not been observed by...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33878-x
更新日期：2018-11-05 00:00:00
abstract：:Controlling light propagation intentionally through turbid media such as ground glass or biological tissue has been demonstrated for many useful applications. Due to random scattering effect, one of the important goals is to draw a desired shape behind turbid media with a swift and precise method. Feedback wavefront s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56214-3
更新日期：2019-12-20 00:00:00
abstract：:Capable of mediating efficient transfection and protein production without eliciting innate immune responses, chemically modified mRNA holds great potential to produce paracrine factors at a physiologically beneficial level, in a spatiotemporally controlled manner, and with low toxicity. Although highly promising in c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35570-6
更新日期：2018-11-30 00:00:00
abstract：:Junction-less nanowire transistors are being investigated to solve short channel effects in future CMOS technology. Here we demonstrate 8 nm diameter silicon nanowire junction-less transistors with metallic doping densities which demonstrate clear 1D electronic transport characteristics. The 1D regime allows excellent...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03138-5
更新日期：2017-06-07 00:00:00
abstract：:The functions of female song found in a few cicadas have rarely been studied. In the cicada Subpsaltria yangi we investigated the acoustic behaviour and signal structure of songs produced by females, the phonotaxis of males, and mate choice, as well as the selective pressure imposed on this species by predators. Pair-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06825-5
更新日期：2017-07-25 00:00:00
abstract：:The rapid increase in use of electronic-cigarettes (e-cigarettes), especially among youth, raises the urgency for regulating bodies to make informed decisions, guidance, and policy on these products. This study evaluated cardiac function in an experimental model following exposure to e-cigarettes. We subjected C57BL/6...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40847-5
更新日期：2019-03-11 00:00:00
abstract：:Efficient hematopoietic reconstitution of wild type mice requires preconditioning. Established experimental protocols exist to transplant hematopoietic stem cells into lethally irradiated or chemically myeloablated adult mice or unirradiated immunodeficient mice. We sought to develop a protocol to reconstitute immuno-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30767-1
更新日期：2018-08-16 00:00:00
abstract：:Motivated from numerous practical applications, the present theoretical and numerical work investigates the nonlinear magnetohydrodynamic (MHD) laminar boundary layer flow of an incompressible, viscous fluid over a porous stretching sheet in the presence of suction/injection (mass transpiration). The flow characterist...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52597-5
更新日期：2019-12-06 00:00:00
abstract：:Fungal pathogens represent a significant threat to immunocompromised patients or individuals with traumatic injury. Strategies to efficiently remove fungal spores from hospital surfaces and, ideally, patient skin thus offer the prospect of dramatically reducing infections in at-risk patients. Photodynamic inactivation...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05000-0
更新日期：2017-07-04 00:00:00
abstract：:Atlantic bluefin tuna (Thunnus thynnus) from the two main spawning populations in the Mediterranean and Gulf of Mexico occur together in the western, central and eastern Atlantic. Stock composition of catches from mixing areas is uncertain, presenting a major challenge to the sustainable management of the fisheries. T...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71355-6
更新日期：2020-09-07 00:00:00
abstract：:The end-Triassic is characterized by one of the largest mass extinctions in the Phanerozoic, coinciding with major carbon cycle perturbations and global warming. It has been suggested that the environmental crisis is linked to widespread sill intrusions during magmatism associated with the Central Atlantic Magmatic Pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18629-8
更新日期：2018-01-09 00:00:00
abstract：:Lithium vanadium phosphate (Li3V2(PO4)3, LVP)/reduced graphene oxide (rGO) composite is prepared with a rheological method followed by heat treatment. The size and interface of LVP particles, two important merits for a cathode material, can be effectively tuned by the rGO in the composite, which plays as surfactant to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05768
更新日期：2014-08-29 00:00:00
abstract：:We performed an integrated analysis of the transcriptome and metabolome from purple (Pr) and normal cultivated varieties (CK) of Dendrobium officinale to gain insights into the regulatory networks associated with phenylpropanoid metabolism and to identify the key regulatory genes of pigmentation. Metabolite and transc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74789-0
更新日期：2020-10-19 00:00:00
abstract：:We consider the finite-temperature properties of the extended Bose-Hubbard model realized recently in an ETH experiment [Nature 532, 476 (2016)]. Competing short- and global-range interactions accommodate fascinating collective phenomena. We formulate a self-consistent mean-field theory to describe the behaviors of th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66054-1
更新日期：2020-06-03 00:00:00
abstract：:The present study investigates the biostimulant effects of 18 Crude Bio-Extracts (CBEs) obtained from Microalgae and Cyanobacteria on tomato plant growth, chlorophyll content, nutrient uptake and metabolite profile. Significant root and shoot length improvement (112.65%, 53.70%); was recorded at treatment with Aphanot...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59840-4
更新日期：2020-02-18 00:00:00
abstract：:Vlasouliolides A-D (1-4), four rare sesquiterpene lactone dimers, were isolated from Vladimiria souliei. The common structural characteristic of 1-4 is the C32 skeleton comprising two sesquiterpene lactone units linked by a C11-C13' single bond with one acetyl connected to the C-13 position of one of the two sesquiter...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43837
更新日期：2017-03-03 00:00:00
abstract：:Gallbladder cancer (GBC) is a highly malignant tumor with poorly understood etiology. An insight into phenotypic features of this malignancy may add to the knowledge of its carcinogenesis and pave the way to new therapeutic approaches. We assessed the expression of female sex hormone receptors (ERα, ERβ, PR), connecti...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58777-y
更新日期：2020-02-05 00:00:00
abstract：:Nitric oxide (NO) signaling has been studied in the eye, including in the pathophysiology of some eye diseases. While NO production by nitric oxide synthase (NOS) enzymes in the eye has been characterized, the more recently described pathways of NO generation by nitrate (NO3-) and nitrite (NO2-) ions reduction has rec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69272-9
更新日期：2020-08-05 00:00:00
abstract：:Quantum bits (qubits) are at the heart of quantum information processing schemes. Currently, solid-state qubits, and in particular the superconducting ones, seem to satisfy the requirements for being the building blocks of viable quantum computers, since they exhibit relatively long coherence times, extremely low diss...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29374
更新日期：2016-07-12 00:00:00
abstract：:The full length human histone 3 lysine 4 demethylase KDM5B (PLU-1/Jarid1B) has been studied using Hydrogen/Deuterium exchange mass spectrometry, homology modelling, sequence analysis, small angle X-ray scattering and electron microscopy. This first structure on an intact multi-domain Jumonji histone demethylase reveal...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40573-y
更新日期：2019-03-11 00:00:00
abstract：:Protoporphyrin IX (PpIX) induced by 5-aminolevulinic acid (5-ALA) is increasingly used as a fluorescent marker for fluorescence-guided resection of malignant gliomas. Understanding how the properties of the excitation light source and PpIX fluorescence interact with the surgical microscope is critical for effective us...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30247-6
更新日期：2018-08-22 00:00:00
abstract：:Insects' exoskeleton, gut, hemocoel, and cells are colonized by various microorganisms that often play important roles in their host life. Moreover, insects are frequently infected by vertically transmitted symbionts that can manipulate their reproduction. The aims of this study were the characterization of bacterial ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32747-x
更新日期：2018-09-26 00:00:00
abstract：:Increased filaggrin expression was found to be correlated with severity scores in chronic spontaneous urticaria (CSU); however, the role of filaggrin breakdown products (FBPs) in CSU has not been studied. We collected stratum corneum (SC) specimens from the volar forearms of 10 CSU patients, 10 AD patients, and 10 hea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01487-9
更新日期：2017-05-02 00:00:00
