Abstract:
PURPOSE:To test a new assay based on an ex vivo cytokine release from whole blood for the monitoring of immune changes in human immunodeficiency virus (HIV)-infected patients. METHODS:A pilot study of outpatients with HIV infection (n = 9) at a large academic hospital who were divided into three groups: HIV-infected patients on highly active antiretroviral therapy (HAART) with a CD4(+) cell count >350/μL (group I) or a CD4(+) cell count <350/μL (group II) and HIV-infected HAART-naïve subjects with a CD4(+) cell count >350/μL (group III). All groups were compared with healthy volunteers (n = 3). The ex vivo cytokine release assay was performed in a three-step process: (1) blood collection, (2) whole-blood ex vivo incubation over 48 h without or with a standard set of well-defined recall antigens as comparable to those used formerly in the skin delayed-type hypersensitivity (DTH) test, (3) cytokine determination from the assay supernatant. RESULTS:Under stimulated conditions, untreated HIV-infected patients with a CD4(+) count >350/μL had similar interleukin-2 (IL-2) levels in the supernatant of the whole-blood incubation to HIV-infected patients on HAART with a low CD4(+) count. Both groups revealed lower IL-2 levels in the supernatant than HIV-infected patients on HAART and with a CD4(+) count >350/μL or healthy volunteers. The determination of interferon-γ and tumour necrosis factor-α in the supernatant showed a similar arrangement of cytokines between groups. CONCLUSIONS:Our results suggest that this cytokine release assay could be a suitable tool to mirror the immunological responsiveness of patients with HIV infection in a gradual manner; further studies are required in order to assess its value in HAART monitoring.
journal_name
Infectionjournal_title
Infectionauthors
Kaufmann I,Draenert R,Gruber M,Feuerecker M,Roider J,Choukèr Adoi
10.1007/s15010-013-0445-8subject
Has Abstractpub_date
2013-06-01 00:00:00pages
687-90issue
3eissn
0300-8126issn
1439-0973journal_volume
41pub_type
杂志文章相关文献
INFECTION文献大全abstract::A 38-year-old Omani woman, seven months pregnant, developed extensive zygomycosis involving maxillary and temporal bones. No evidence of any underlying immune deficiency was detected except that which may be attributed to pregnancy. After delivery the patient was treated with repeated courses of amphotericin B, which ...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF02033066
更新日期:1992-07-01 00:00:00
abstract:BACKGROUND:The aim of the study was to evaluate the efficacy and safety of combined treatment with natural leukocyte interferon alpha (Alfaferone) plus ribavirin in patients with HCV-related cirrhosis. PATIENTS AND METHODS:Twenty-three patients (15 women, 8 men) aged 17-68 years hospitalized in 2005-2008 were included...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/s15010-011-0135-3
更新日期:2011-10-01 00:00:00
abstract:PURPOSE:The main purpose of this study was to examine the overall distribution of chronic comorbidities in coronavirus disease-19 (COVID-19) infected populations and the risk of the underlying burden of disease in terms of the case fatality ratio (CFR). METHODS:We carried out a systematic review and meta-analysis of s...
journal_title:Infection
pub_type: 杂志文章,meta分析
doi:10.1007/s15010-020-01502-8
更新日期:2020-12-01 00:00:00
abstract::A microbiological analysis of 102 patients suffering from cystic fibrosis was conducted over a 22 month period. 20 microbial species with the following incidence were identified: Pseudomonas aeruginosa: 83.4%; Candida albicans: 29.4%; Staphylococcus aureus: 24.5%; Staphylococcus epidermidis: 11.8%; Haemophilus influen...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01644137
更新日期:1987-07-01 00:00:00
abstract::Cefoperazone, an antibiotic commonly used for prophylaxis of infection, has been associated with hypoprothrombinemia and bleeding. To reduce the risk of bleeding, co-administration of vitamin K has been advised. We reassessed the need for vitamin K use in a retrospective analysis of 50 patients undergoing urologic pro...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01704604
更新日期:1992-05-01 00:00:00
abstract::To detect microorganisms in the blood it is necessary not only that the microbiologist uses reliable methods, but also that the clinician takes a sufficient number of blood samples at the right point in time using a correct method for drawing the blood. The best results are obtained if the blood sample is transferred ...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01639113
更新日期:1977-03-01 00:00:00
abstract:PURPOSE:Limited data are available on immunologic responses to primary pandemic H1N1 (2009) vaccination in recipients of allogeneic hematopoietic stem cell transplantation (HSCT) recipients. In 2009 serologic responses to either pandemic H1N1 (2009) vaccine (n = 36) or pandemic H1N1 (2009) infection (n = 2) were studie...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/s15010-011-0206-5
更新日期:2012-04-01 00:00:00
abstract::A case of appendicitis followed by reactive arthritis in an HLA B27-positive, 29-year-old man after infection with Yersinia enterocolitica is reported. Infection with Y. enterocolitica was diagnosed by determination of serotype specific antibodies and antibodies to Yersinia outer membrane proteins. Bacteriological cul...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01720409
更新日期:1997-09-01 00:00:00
abstract::Penetration of ciprofloxacin into human tonsils was studied following an intravenous infusion of 200 mg over 15 minutes to adult humans undergoing tonsillectomy. Samples were taken one-and-a-half to four hours after dosing. Generally, tissue levels exceeded corresponding serum concentrations by 50% (range of intraindi...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01651152
更新日期:1984-09-01 00:00:00
abstract::The activity of clarithromycin in combination with ethambutol and rifampicin was tested at concentrations achievable in serum against 20 strains of Mycobacterium avium complex. The combination of clarithromycin plus rifampicin showed inhibition of 11 of the strains and bactericidal synergism against seven of the strai...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01645362
更新日期:1991-09-01 00:00:00
abstract::The in vitro activity of the free acid of cefetamet pivoxil (Ro 15-8075) was tested against 355 clinical isolates, namely enteropathogenic bacteria, glucose non-fermentative gram-negative rods (excluding Pseudomonas aeruginosa) and Legionella pneumophila. Ceftriaxone was included in the study as reference compound. Al...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01644102
更新日期:1988-05-01 00:00:00
abstract::Influenza viruses type A and B can cause a wide spectrum of illness, and they are responsible for considerable mortality and morbidity. With the new neuraminidase inhibitors, of which zanamivir was the first drug to be licensed, the physician has antivirals at his disposal which are safe and effective against both inf...
journal_title:Infection
pub_type: 杂志文章,评审
doi:10.1007/s150100070016
更新日期:2000-09-01 00:00:00
abstract:PURPOSE:Cardiac involvement in dengue fever is underdiagnosed due to low index of clinical suspicion and its contribution to hemodynamic instability in severe dengue is not well known. METHODS:A prospective observational study was conducted among admitted patients ≥ 14 years of age having confirmed dengue fever. Patie...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/s15010-020-01500-w
更新日期:2020-12-01 00:00:00
abstract:PURPOSE:The Choosing Wisely® initiative is an international campaign addressing over- and underuse of diagnostic and therapeutic measures in infectious diseases among others. Since 2016, the German Society for Infectious Diseases (DGI) has constantly designed new items in this regard. Here we report the most recent rec...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/s15010-020-01400-z
更新日期:2020-06-01 00:00:00
abstract:: ...
journal_title:Infection
pub_type: 社论
doi:10.1007/s15010-018-1228-z
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:There is increasing evidence indicating an association between invasive non-typeable Haemophilus influenzae (NTHi) infection in pregnancy and early pregnancy loss. As the diagnosis relies on microbiological investigation of post-mortem placental and foetal samples, a significant proportion of NTHi-related pr...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/s15010-019-01359-6
更新日期:2020-04-01 00:00:00
abstract::A guinea pig model of experimental Pseudomonas aeruginosa pneumonia was used to evaluate factors affecting the efficacy of passive immune therapy with Psomaglobin N, a hyperimmune P. aeruginosa globulin. Animals treated 2 h after infection with a single intravenous infusion of Psomaglobin N, 500 mg/kg, demonstrated 33...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01644192
更新日期:1987-01-01 00:00:00
abstract::To investigate the diagnostic value of a direct immunofluorescence test (DIF-test), urethral samples and first catch urine (FCU) from 153 male patients attending an outpatient clinic for sexually transmitted diseases (STD) were studied. Of the male patients, 40 (26.1%) had a positive urethral culture, 39 (25.5%) had a...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01711077
更新日期:1992-03-01 00:00:00
abstract::The concentrations of ciprofloxacin produced in bone, cartilage and menisci after a single administration of 200 mg were determined at different intervals in a group of patients with an average age of 80 years. Concentrations of 0.11 to 0.94 mg/kg bone tissue were measured after 0.5 to 5 hours. In the cartilage a conc...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01642108
更新日期:1990-05-01 00:00:00
abstract:PURPOSE:To assess whether diabetes is a risk factor for herpes zoster (HZ). METHODS:We conducted a retrospective cohort study using the Integrated Health Care Information Services database, during the period 1997-2006. A type I diabetes cohort, a type II diabetes cohort and two non-diabetic cohorts matched for date of...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/s15010-014-0645-x
更新日期:2014-08-01 00:00:00
abstract::The HBeAg/anti-HBe system was studied as a marker of infectivity and chronic progressive liver disease in 460 hemodialysis patients. The importance of HBeAg as an index of infectivity was confirmed in that it was present simultaneously with specific DNA polymerase (31 patients) and by the presence of widely diffuse co...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01641020
更新日期:1984-01-01 00:00:00
abstract::The incidence of urinary tract infections (UTI) in 299 renal graft transplantations (281 patients) was analyzed. UTI episodes were demonstrated in 185 grafts (62%), most frequently in the first month after transplantation. The infectious episodes were mostly recurrent. Persistent infection, detected in 11% of grafts, ...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01667212
更新日期:1985-09-01 00:00:00
abstract::The in vitro activity of clarithromycin alone and in combination with its primary human metabolite, 14-hydroxy-clarithromycin, was determined against 203 strains of Haemophilus influenzae. Microdilution broth MICs and MBCs of both clarithromycin and 14-hydroxy-clarithromycin were determined. The clarithromycin MIC50 w...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01704612
更新日期:1992-05-01 00:00:00
abstract:BACKGROUND:Infections caused by bacteria are a foremost cause of morbidity and mortality in the world. The common strategy of treating bacterial infections is by local or systemic administration of antimicrobial agents. Currently, the increasing antibiotic resistance is a serious and global problem. Since the most impo...
journal_title:Infection
pub_type: 杂志文章,评审
doi:10.1007/s15010-018-1222-5
更新日期:2019-02-01 00:00:00
abstract:PURPOSE:Raoultella ornithinolytica is not well known as a clinical pathogen. We performed a retrospective review of R. ornithinolytica bacteremia to investigate its clinical features, antimicrobial susceptibility, and overall patient outcomes. METHODS:R. ornithinolytica bacteremia cases were collected from an electron...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/s15010-014-0696-z
更新日期:2015-02-01 00:00:00
abstract::The ability of clavulanic acid to inactivate beta-lactamase was investigated using 47 amoxicillin-resistant bacteria. In the presence of 10 mg/l clavulanic acid most strains of staphylococci, Escherichia coli, Klebsiella and Proteus mirabilis became fully amoxicillin-sensitive. This effect of clavulanic acid was in mo...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01641472
更新日期:1980-01-01 00:00:00
abstract:OBJECTIVE:"Choosing Wisely" is a growing international campaign aiming at practice changes to improve patient health and safety by both, conduct of essential and avoidance of unnecessary diagnostic, preventive and therapeutic procedures. The goal is to create an easily recognizable and distributable list ("Choosing Wis...
journal_title:Infection
pub_type: 杂志文章,评审
doi:10.1007/s15010-017-0997-0
更新日期:2017-06-01 00:00:00
abstract:PURPOSE:Since May 2016, WHO recommended a 9-12 month short-treatment regimen for multidrug-resistant tuberculosis (MDR-TB) treatment known as the 'Bangladesh Regimen'. However, limited data exist on the appropriateness thereof, and its implementation in low- and middle-income countries (LMIC). We report here on the pil...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/s15010-019-01314-5
更新日期:2019-10-01 00:00:00
abstract::The influence of cefodizime (CDZ) on CD4 and CD8 lymphocytes was investigated in patients with lower respiratory tract infection and underlying respiratory diseases. Ten men and one woman were treated with CDZ 1 g i.m. b.i.d. for ten days. The infecting organisms were Haemophilus influenzae (5), Streptococcus pneumoni...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01709955
更新日期:1992-01-01 00:00:00
abstract::A comparison was made between the Septi-Chek "Release" system containing saponins, standard BacT/Alert blood culture bottles and FAN BacT/Alert blood culture bottles for culturing CAPD fluids. Seventy-seven CAPD effluent specimens were tested. No differences could be found. Therefore, lytic agents are not necessary wh...
journal_title:Infection
pub_type: 杂志文章
doi:10.1007/BF01713570
更新日期:1995-11-01 00:00:00