A new cytokine release assay: a simple approach to monitor the immune status of HIV-infected patients.

Abstract:

PURPOSE:To test a new assay based on an ex vivo cytokine release from whole blood for the monitoring of immune changes in human immunodeficiency virus (HIV)-infected patients. METHODS:A pilot study of outpatients with HIV infection (n = 9) at a large academic hospital who were divided into three groups: HIV-infected patients on highly active antiretroviral therapy (HAART) with a CD4(+) cell count >350/μL (group I) or a CD4(+) cell count <350/μL (group II) and HIV-infected HAART-naïve subjects with a CD4(+) cell count >350/μL (group III). All groups were compared with healthy volunteers (n = 3). The ex vivo cytokine release assay was performed in a three-step process: (1) blood collection, (2) whole-blood ex vivo incubation over 48 h without or with a standard set of well-defined recall antigens as comparable to those used formerly in the skin delayed-type hypersensitivity (DTH) test, (3) cytokine determination from the assay supernatant. RESULTS:Under stimulated conditions, untreated HIV-infected patients with a CD4(+) count >350/μL had similar interleukin-2 (IL-2) levels in the supernatant of the whole-blood incubation to HIV-infected patients on HAART with a low CD4(+) count. Both groups revealed lower IL-2 levels in the supernatant than HIV-infected patients on HAART and with a CD4(+) count >350/μL or healthy volunteers. The determination of interferon-γ and tumour necrosis factor-α in the supernatant showed a similar arrangement of cytokines between groups. CONCLUSIONS:Our results suggest that this cytokine release assay could be a suitable tool to mirror the immunological responsiveness of patients with HIV infection in a gradual manner; further studies are required in order to assess its value in HAART monitoring.

journal_name

Infection

journal_title

Infection

authors

Kaufmann I,Draenert R,Gruber M,Feuerecker M,Roider J,Choukèr A

doi

10.1007/s15010-013-0445-8

subject

Has Abstract

pub_date

2013-06-01 00:00:00

pages

687-90

issue

3

eissn

0300-8126

issn

1439-0973

journal_volume

41

pub_type

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