Quantitative assessment of T cell repertoire recovery after hematopoietic stem cell transplantation.

Abstract:

:Delayed T cell recovery and restricted T cell receptor (TCR) diversity after allogeneic hematopoietic stem cell transplantation (allo-HSCT) are associated with increased risks of infection and cancer relapse. Technical challenges have limited faithful measurement of TCR diversity after allo-HSCT. Here we combined 5' rapid amplification of complementary DNA ends PCR with deep sequencing to quantify TCR diversity in 28 recipients of allo-HSCT using a single oligonucleotide pair. Analysis of duplicate blood samples confirmed that we accurately determined the frequency of individual TCRs. After 6 months, cord blood-graft recipients approximated the TCR diversity of healthy individuals, whereas recipients of T cell-depleted peripheral-blood stem cell grafts had 28-fold and 14-fold lower CD4(+) and CD8(+) T cell diversities, respectively. After 12 months, these deficiencies had improved for the CD4(+) but not the CD8(+) T cell compartment. Overall, this method provides unprecedented views of T cell repertoire recovery after allo-HSCT and may identify patients at high risk of infection or relapse.

journal_name

Nat Med

journal_title

Nature medicine

authors

van Heijst JW,Ceberio I,Lipuma LB,Samilo DW,Wasilewski GD,Gonzales AM,Nieves JL,van den Brink MR,Perales MA,Pamer EG

doi

10.1038/nm.3100

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

372-7

issue

3

eissn

1078-8956

issn

1546-170X

pii

nm.3100

journal_volume

19

pub_type

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