Recombinant Ganoderma lucidum immunomodulatory protein modified with polyethylene glycol.

Abstract:

:Recombinant Ganoderma lucidum immunomodu-latory protein (rLZ-8) expressed using the Pichia yeast eukaryotic expression system is a potential new drug for cancer therapy; however, it has a short half-life in the body. In order to optimize the potency and stability of rLZ-8, we modified the recombinant protein chemically using methoxy-PEG-succinimidyl propionate (mPEG-SPA). The results indicated that several parameters, including pH, the molar ratio of rLZ-8 to mPEG-SPA and time, played crucial roles in the modification process. In particular, when the molar ratio of rLZ-8 to mPEG-SPA was 1:1, rLZ-8 was modified by a single mPEG-SPA moiety. In addition, MALDI-TOF/TOF and ESI Q-Trap results revealed that the difference in molecular weight (MW) between the peptide-linked mPEG-SPA and the mPEG-SPA closely matched the MW of a methionine amino acid. Taken together, these data suggest that modification of mPEG-SPA occurred on the N-terminal helix of rLZ-8. This modification method has laid a foundation for the development of long-acting formulations of rLZ-8.

journal_name

Mol Med Rep

authors

Zhang X,Sun F,Liu Z,Zhang S,Liang C

doi

10.3892/mmr.2013.1281

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

975-80

issue

3

eissn

1791-2997

issn

1791-3004

journal_volume

7

pub_type

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