Abstract:
:Borrelia burgdorferi, the agent of Lyme disease, has cholesterol and cholesterol-glycolipids that are essential for bacterial fitness, are antigenic, and could be important in mediating interactions with cells of the eukaryotic host. We show that the spirochetes can acquire cholesterol from plasma membranes of epithelial cells. In addition, through fluorescent and confocal microscopy combined with biochemical approaches, we demonstrated that B. burgdorferi labeled with the fluorescent cholesterol analog BODIPY-cholesterol or (3)H-labeled cholesterol transfer both cholesterol and cholesterol-glycolipids to HeLa cells. The transfer occurs through two different mechanisms, by direct contact between the bacteria and eukaryotic cell and/or through release of outer membrane vesicles. Thus, two-way lipid exchange between spirochetes and host cells can occur. This lipid exchange could be an important process that contributes to the pathogenesis of Lyme disease.
journal_name
PLoS Pathogjournal_title
PLoS pathogensauthors
Crowley JT,Toledo AM,LaRocca TJ,Coleman JL,London E,Benach JLdoi
10.1371/journal.ppat.1003109subject
Has Abstractpub_date
2013-01-01 00:00:00pages
e1003109issue
1eissn
1553-7366issn
1553-7374pii
PPATHOGENS-D-12-02049journal_volume
9pub_type
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