In vitro cytotoxicity of fluorescent silica nanoparticles hybridized with aggregation-induced emission luminogens for living cell imaging.

Abstract:

:Fluorescent silica nanoparticles (FSNPs) can provide high-intensity and photostable fluorescent signals as a probe for biomedical analysis. In this study, FSNPs hybridized with aggregation-induced emission (AIE) luminogens (namely FSNP-SD) were successfully fabricated by a surfactant-free sol-gel method. The FSNP-SD were spherical, monodisperse and uniform in size, with an average diameter of approximately 100 nm, and emitted strong fluorescence at the peak of 490 nm. The FSNP-SD selectively stained the cytoplasmic regions and were distributed in the cytoplasm. Moreover, they can stay inside cells, enabling the tacking of cells over a long period of time. The intracellular vesicles and multinucleated cells were increase gradually with the rise of FSNP-SD concentration. Both cell viability and survival only lost less than 20% when the cells were exposed to the high concentration of 100 μg/mL FSNP-SD. Additionally, the cell apoptosis and intracellular ROS assay indicated that FSNP-SD had no significant toxic effects at the maximum working concentration of 80 μg/mL. This study demonstrated that the FSNP-SD are promising biocompatible fluorescent probes for living cell imaging.

journal_name

Int J Mol Sci

authors

Xia Y,Li M,Peng T,Zhang W,Xiong J,Hu Q,Song Z,Zheng Q

doi

10.3390/ijms14011080

subject

Has Abstract

pub_date

2013-01-07 00:00:00

pages

1080-92

issue

1

issn

1422-0067

pii

ijms14011080

journal_volume

14

pub_type

杂志文章
  • Twist2 Is Upregulated in Early Stages of Repair Following Acute Kidney Injury.

    abstract::The aging kidney is a marked by a number of structural and functional changes, including an increased susceptibility to acute kidney injury (AKI). Previous studies from our laboratory have shown that aging male Fischer 344 rats (24 month) are more susceptible to apoptosis-mediated injury than young counterparts. In th...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18020368

    authors: Grunz-Borgmann EA,Nichols LA,Wang X,Parrish AR

    更新日期:2017-02-10 00:00:00

  • Apelin Controls Angiogenesis-Dependent Glioblastoma Growth.

    abstract::Glioblastoma (GBM) present with an abundant and aberrant tumor neo-vasculature. While rapid growth of solid tumors depends on the initiation of tumor angiogenesis, GBM also progress by infiltrative growth and vascular co-option. The angiogenic factor apelin (APLN) and its receptor (APLNR) are upregulated in GBM patien...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21114179

    authors: Frisch A,Kälin S,Monk R,Radke J,Heppner FL,Kälin RE

    更新日期:2020-06-11 00:00:00

  • In Vitro Expansion of CAG, CAA, and Mixed CAG/CAA Repeats.

    abstract::Polyglutamine diseases, including Huntington's disease and a number of spinocerebellar ataxias, are caused by expanded CAG repeats that are located in translated sequences of individual, functionally-unrelated genes. Only mutant proteins containing polyglutamine expansions have long been thought to be pathogenic, but ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms160818741

    authors: Figura G,Koscianska E,Krzyzosiak WJ

    更新日期:2015-08-11 00:00:00

  • Consequences of morphology on molecularly imprinted polymer-ligand recognition.

    abstract::The relationship between molecularly imprinted polymer (MIP) morphology and template-rebinding over a series of warfarin-imprinted methacrylic acid co(ethylene dimethacrylate) polymers has been explored. Detailed investigations of the nature of template recognition revealed that an optimal template binding was obtain...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms14011207

    authors: Rosengren AM,Karlsson BC,Nicholls IA

    更新日期:2013-01-09 00:00:00

  • Estrogen Effects on Wound Healing.

    abstract::Wound healing is a physiological process, involving three successive and overlapping phases-hemostasis/inflammation, proliferation, and remodeling-to maintain the integrity of skin after trauma, either by accident or by procedure. Any disruption or unbalanced distribution of these processes might result in abnormal wo...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18112325

    authors: Horng HC,Chang WH,Yeh CC,Huang BS,Chang CP,Chen YJ,Tsui KH,Wang PH

    更新日期:2017-11-03 00:00:00

  • Comparison of Antibacterial Adhesion When Salivary Pellicle Is Coated on Both Poly(2-hydroxyethyl-methacrylate)- and Polyethylene-glycol-methacrylate-grafted Poly(methyl methacrylate).

    abstract::Although poly(2-hydroxyethyl methacrylate) (pHEMA) and polyethylene glycol methacrylate (PEGMA) have been demonstrated to inhibit bacterial adhesion, no study has compared antibacterial adhesion when salivary pellicle is coated on polymethyl methacrylate (PMMA) grafted with pHEMA and on PMMA grafted with PEGMA. In thi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19092764

    authors: Lee BS,Chen YJ,Wei TC,Ma TL,Chang CC

    更新日期:2018-09-14 00:00:00

  • HER-2 Expression in Brain Metastases from Colorectal Cancer and Corresponding Primary Tumors: A Case Cohort Series.

    abstract::Brain metastases (BM) from colorectal cancer (CRC) are a rare but increasing event. Surgical resection of oligometastatic disease, including BM, may produce a survival benefit in selected patients. Previous studies described the HER-2 expression patterns in CRC patients, but its prognostic role still remains controver...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms14022370

    authors: Aprile G,De Maglio G,Menis J,Casagrande M,Tuniz F,Pisa EF,Fontanella C,Skrap M,Beltrami AC,Fasola G,Pizzolitto S

    更新日期:2013-01-24 00:00:00

  • The Impact of Genetic Polymorphisms in Organic Cation Transporters on Renal Drug Disposition.

    abstract::A considerable number of drugs and/or their metabolites are excreted by the kidneys through glomerular filtration and active renal tubule secretion via transporter proteins. Uptake transporters in the proximal tubule are part of the solute carrier (SLC) superfamily, and include the organic cation transporters (OCTs). ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21186627

    authors: Zazuli Z,Duin NJCB,Jansen K,Vijverberg SJH,Maitland-van der Zee AH,Masereeuw R

    更新日期:2020-09-10 00:00:00

  • Can NF-κB Be Considered a Valid Drug Target in Neoplastic Diseases? Our Point of View.

    abstract::Multidrug resistance (MDR), of the innate and acquired types, is one of major problems in treating tumor diseases with a good chance of success. In this review, we examine the key role of nuclear factor-kappa B (NF-κB) to induce MDR in three tumor models characterized precisely by innate or acquired MDR, in particular...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21093070

    authors: Labbozzetta M,Notarbartolo M,Poma P

    更新日期:2020-04-27 00:00:00

  • Heterogeneous Network Model to Identify Potential Associations Between Plasmodium vivax and Human Proteins.

    abstract::Integration of multiple sources and data levels provides a great insight into the complex associations between human and malaria systems. In this study, a meta-analysis framework was developed based on a heterogeneous network model for integrating human-malaria protein similarities, a human protein interaction network...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21041310

    authors: Suratanee A,Plaimas K

    更新日期:2020-02-15 00:00:00

  • JNK Signaling as a Key Modulator of Soft Connective Tissue Physiology, Pathology, and Healing.

    abstract::In healthy individuals, the healing of soft tissues such as skin after pathological insult or post injury follows a relatively predictable and defined series of cell and molecular processes to restore tissue architecture and function(s). Healing progresses through the phases of hemostasis, inflammation, proliferation,...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21031015

    authors: Nikoloudaki G,Brooks S,Peidl AP,Tinney D,Hamilton DW

    更新日期:2020-02-04 00:00:00

  • The Olfactory System of Zebrafish as a Model for the Study of Neurotoxicity and Injury: Implications for Neuroplasticity and Disease.

    abstract::The olfactory system, composed of the olfactory organs and the olfactory bulb, allows organisms to interact with their environment and through the detection of odor signals. Olfaction mediates behaviors pivotal for survival, such as feeding, mating, social behavior, and danger assessment. The olfactory organs are dire...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20071639

    authors: Calvo-Ochoa E,Byrd-Jacobs CA

    更新日期:2019-04-02 00:00:00

  • It Is Imperative to Establish a Pellucid Definition of Chimeric RNA and to Clear Up a Lot of Confusion in the Relevant Research.

    abstract::There have been tens of thousands of RNAs deposited in different databases that contain sequences of two genes and are coined chimeric RNAs, or chimeras. However, "chimeric RNA" has never been lucidly defined, partly because "gene" itself is still ill-defined and because the means of production for many RNAs is unclea...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18040714

    authors: Yuan C,Han Y,Zellmer L,Yang W,Guan Z,Yu W,Huang H,Liao DJ

    更新日期:2017-03-28 00:00:00

  • Sunflower-based feedstocks in nonfood applications: Perspectives from olefin metathesis.

    abstract::Sunflower (Helianthus annuus L.) oil remains under-utilised albeit one of the major seed oils produced world-wide. Moreover, the high oleic sunflower varieties make the oil attractive for applications requiring high temperature processes and those targeting the C=C double bond functionality. Herein an overview of the ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms9081393

    authors: Marvey BB

    更新日期:2008-08-01 00:00:00

  • Establishing Computational Approaches Towards Identifying Malarial Allosteric Modulators: A Case Study of Plasmodium falciparum Hsp70s.

    abstract::Combating malaria is almost a never-ending battle, as Plasmodium parasites develop resistance to the drugs used against them, as observed recently in artemisinin-based combination therapies. The main concern now is if the resistant parasite strains spread from Southeast Asia to Africa, the continent hosting most malar...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20225574

    authors: Amusengeri A,Astl L,Lobb K,Verkhivker GM,Tastan Bishop Ö

    更新日期:2019-11-08 00:00:00

  • The Application of Heptamethine Cyanine Dye DZ-1 and Indocyanine Green for Imaging and Targeting in Xenograft Models of Hepatocellular Carcinoma.

    abstract::Near infrared fluorescence (NIRF) imaging has strong potential for widespread use in noninvasive tumor imaging. Indocyanine green (ICG) is the only Food and Drug Administration (FDA) -approved NIRF dye for clinical diagnosis; however, it is unstable and poorly targets tumors. DZ-1 is a novel heptamethine cyanine NIRF ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18061332

    authors: Zhang C,Zhao Y,Zhang H,Chen X,Zhao N,Tan D,Zhang H,Shi C

    更新日期:2017-06-21 00:00:00

  • Small Molecules and Peptides Targeting Glial Cell Line-Derived Neurotrophic Factor Receptors for the Treatment of Neurodegeneration.

    abstract::Glial cell line-derived neurotrophic factor (GDNF) family ligands (GFLs) are able to promote the survival of multiple neuronal populations in the body and, therefore, hold considerable promise for disease-modifying treatments of diseases and conditions caused by neurodegeneration. Available data reveal the potential o...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21186575

    authors: Sidorova YA,Saarma M

    更新日期:2020-09-08 00:00:00

  • Gelam honey has a protective effect against lipopolysaccharide (LPS)-induced organ failure.

    abstract::Gelam honey exerts anti-inflammatory and antioxidant activities and is thought to have potent effects in reducing infections and healing wounds. The aim of this study was to investigate the effects of intravenously-injected Gelam honey in protecting organs from lethal doses of lipopolysaccharide (LPS). Six groups of r...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms13056370

    authors: Kassim M,Mansor M,Al-Abd N,Yusoff KM

    更新日期:2012-01-01 00:00:00

  • Time Response of Oxidative/Nitrosative Stress and Inflammation in LPS-Induced Endotoxaemia-A Comparative Study of Mice and Rats.

    abstract::Sepsis is a severe and multifactorial disease with a high mortality rate. It represents a strong inflammatory response to an infection and is associated with vascular inflammation and oxidative/nitrosative stress. Here, we studied the underlying time responses in the widely used lipopolysaccharide (LPS)-induced endoto...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18102176

    authors: Steven S,Dib M,Roohani S,Kashani F,Münzel T,Daiber A

    更新日期:2017-10-18 00:00:00

  • Early Exercise Protects against Cerebral Ischemic Injury through Inhibiting Neuron Apoptosis in Cortex in Rats.

    abstract::Early exercise is an effective strategy for stroke treatment, but the underlying mechanism remains poorly understood. Apoptosis plays a critical role after stroke. However, it is unclear whether early exercise inhibits apoptosis after stroke. The present study investigated the effect of early exercise on apoptosis ind...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms14036074

    authors: Zhang P,Zhang Y,Zhang J,Wu Y,Jia J,Wu J,Hu Y

    更新日期:2013-03-15 00:00:00

  • The Role of the IL-23/IL-17 Pathway in the Pathogenesis of Spondyloarthritis.

    abstract::Spondyloarthritis (SpA) is a subset of seronegative rheumatic-related autoimmune diseases that consist of ankylosing spondylitis (AS), psoriatic spondylitis (PsA), reactive spondylitis (re-SpA), inflammatory bowel disease (IBD)-associated spondylitis, and unclassifiable spondylitis. These subsets share clinical phenot...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21176401

    authors: Tsukazaki H,Kaito T

    更新日期:2020-09-03 00:00:00

  • Metagenomic Analysis of Upwelling-Affected Brazilian Coastal Seawater Reveals Sequence Domains of Type I PKS and Modular NRPS.

    abstract::Marine environments harbor a wide range of microorganisms from the three domains of life. These microorganisms have great potential to enable discovery of new enzymes and bioactive compounds for industrial use. However, only ~1% of microorganisms from the environment can currently be identified through cultured isolat...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms161226101

    authors: Cuadrat RR,Cury JC,Dávila AM

    更新日期:2015-11-27 00:00:00

  • Coxiella burnetii Lipopolysaccharide: What Do We Know?

    abstract::A small gram-negative bacterium, Coxiella burnetii (C. burnetii), is responsible for a zoonosis called Q fever. C. burnetii is an intracellular bacterium that can survive inside microbicidal cells like monocytes and macrophages by hijacking several functions of the immune system. Among several virulence factors, the l...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18122509

    authors: Abnave P,Muracciole X,Ghigo E

    更新日期:2017-11-23 00:00:00

  • Design, synthesis and antiviral activity studies of schizonepetin derivatives.

    abstract::A series of schizonepetin derivatives have been designed and synthesized in order to obtain potent antivirus agents. The antiviral activity against HSV-1 and influenza virus H3N2 as well as the cytotoxicity of these derivatives was evaluated by using cytopathic effect (CPE) inhibition assay in vitro. Compounds M2, M4,...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms140817193

    authors: Bao B,Meng Z,Li N,Meng Z,Zhang L,Cao Y,Yao W,Shan M,Ding A

    更新日期:2013-08-20 00:00:00

  • Eicosapentaenoic acid protects against palmitic acid-induced endothelial dysfunction via activation of the AMPK/eNOS pathway.

    abstract::Recent studies have shown that free fatty acids are associated with chronic inflammation, which may be involved in vascular injury. The intake of eicosapentaenoic acid (EPA) can decrease cardiovascular disease risks, but the protective mechanisms of EPA on endothelial cells remain unclear. In this study, primary human...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms150610334

    authors: Lee CH,Lee SD,Ou HC,Lai SC,Cheng YJ

    更新日期:2014-06-10 00:00:00

  • Associations of MTHFR C677T and MTRR A66G gene polymorphisms with metabolic syndrome: a case-control study in Northern China.

    abstract::Prior evidence indicates that homocysteine plays a role in the development of metabolic syndrome (MetS). Methylenetetrahydrofolate reductase (MTHFR) C677T and methionine synthase reductase (MTRR) A66G polymorphisms are common genetic determinants of homocysteine levels. To investigate the associations of the MTHFR C67...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms151221687

    authors: Yang B,Fan S,Zhi X,Wang D,Li Y,Wang Y,Wang Y,Wei J,Zheng Q,Sun G

    更新日期:2014-11-25 00:00:00

  • Special Issue of International Journal of Molecular Sciences (IJMS) "Purinergic P2 Receptors: Structure and Function".

    abstract::This Special Issue of International Journal of Molecular Sciences (IJMS) contains 7 reviews and 12 original research papers written by a panel of experts who highlight recent advances in molecular structure and cellular function of purinergic P2 receptors[...]. ...

    journal_title:International journal of molecular sciences

    pub_type: 社论

    doi:10.3390/ijms22010383

    authors: Zemkova H

    更新日期:2020-12-31 00:00:00

  • Trypanosoma brucei Interaction with Host: Mechanism of VSG Release as Target for Drug Discovery for African Trypanosomiasis.

    abstract::The protozoan Trypanosoma brucei, responsible for animal and human trypanosomiasis, has a family of major surface proteases (MSPs) and phospholipase-C (PLC), both involved in some mechanisms of virulence during mammalian infections. During parasitism in the mammalian host, this protozoan is exclusively extracellular a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20061484

    authors: Moreno CJG,Temporão A,Torres T,Sousa Silva M

    更新日期:2019-03-25 00:00:00

  • The Decrease in Human Endogenous Retrovirus-H Activity Runs in Parallel with Improvement in ADHD Symptoms in Patients Undergoing Methylphenidate Therapy.

    abstract::Increasing scientific evidence demonstrated the deregulation of human endogenous retroviruses (HERVs) expression in complex diseases, such as cancer, autoimmune, psychiatric, and neurological disorders. The dynamic regulation of HERV activity and their responsiveness to a variety of environmental stimuli designate HER...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19113286

    authors: Cipriani C,Pitzianti MB,Matteucci C,D'Agati E,Miele MT,Rapaccini V,Grelli S,Curatolo P,Sinibaldi-Vallebona P,Pasini A,Balestrieri E

    更新日期:2018-10-23 00:00:00

  • Preparation of Radiation Cross-Linked Poly(Acrylic Acid) Hydrogel Containing Metronidazole with Enhanced Antibacterial Activity.

    abstract::Metronidazole (MD) is known as a periodontitis medicine and has been widely used in antibiotics for resistance to anaerobic bacteria, periodontal disease, and other threats. To treat diseases, drug delivery carriers are needed with a high bioadhesive property and enhanced drug penetration. Poly (acrylic acid) (PAA) hy...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21010187

    authors: Jeong JO,Park JS,Kim EJ,Jeong SI,Lee JY,Lim YM

    更新日期:2019-12-26 00:00:00