Determination of N-acetylaspartic acid in human cerebrospinal fluid by gas chromatography-mass spectrometry.

abstract：:Specific antisera were raised against the A and B chains of glycoprotein III. Immunoblotting revealed that in adrenal medulla both chains migrate very closely together in two-dimensional electrophoresis. Both chains with slightly differing molecular sizes are found in several endocrine tissues and in brain, kidney, li...

journal_title：Journal of neurochemistry

authors： Laslop A,Steiner HJ,Egger C,Wolkersdorfer M,Kapelari S,Hogue-Angeletti R,Erickson JD,Fischer-Colbrie R,Winkler H

更新日期：1993-10-01 00:00:00

abstract：:Malonaldehyde formation by cortical brain slices from rat brain was determined as a function of incubation time and of oxygen pressure. This substance, a byproduce of lipid peroxidation, was detected by the thiobarbituric acid test. Significant amounts of malonaldehyde were formed by brain slices during incubation in ...

journal_title：Journal of neurochemistry

authors： Kovachich GB,Mishra OP

更新日期：1980-12-01 00:00:00

abstract：:The cDNA for Tyr-Loc, a G protein-coupled receptor that clearly shows homology to a number of mammalian and fruit fly receptors for biogenic amines, was cloned from the nervous system of Locusta migratoria. Functional expression of the cloned cDNA was obtained in cultured insect cells, i.e., in Spodoptera SF9 cells us...

journal_title：Journal of neurochemistry

authors： Vanden Broeck J,Vulsteke V,Huybrechts R,De Loof A

更新日期：1995-06-01 00:00:00

abstract：:We recently demonstrated that the presenilin-dependent gamma-secretase complex regulates the expression and activity of neprilysin, one of the main enzymes that degrade the amyloid beta-peptide (Abeta) which accumulates in Alzheimer's disease. Here, we examined the influence of endogenous nicastrin (NCT), a member of ...

journal_title：Journal of neurochemistry

authors： Pardossi-Piquard R,Dunys J,Yu G,St George-Hyslop P,Alves da Costa C,Checler F

更新日期：2006-05-01 00:00:00

abstract：:Acute exposure to 100 mM isotonic ethanol (EtOH) increased intracellular Ca2+ concentration ([Ca2+]i), induced cell swelling, and transformed actin cytoskeleton in astroglial primary cultures from rat cerebral cortex. Fluorometric recordings of fluo-3AM- or fura-2AM-incubated astroglial cells revealed that EtOH induce...

journal_title：Journal of neurochemistry

authors： Allansson L,Khatibi S,Olsson T,Hansson E

更新日期：2001-01-01 00:00:00

abstract：:Certain non-steroidal anti-inflammatory drugs (NSAIDs) preferentially inhibit production of the amyloidogenic Abeta42 peptide, presumably by direct modulation of gamma-secretase activity. A recent report indicated that NSAIDs could reduce Abeta42 by inhibition of the small GTPase Rho, and a single inhibitor of Rho kin...

journal_title：Journal of neurochemistry

authors： Leuchtenberger S,Kummer MP,Kukar T,Czirr E,Teusch N,Sagi SA,Berdeaux R,Pietrzik CU,Ladd TB,Golde TE,Koo EH,Weggen S

更新日期：2006-01-01 00:00:00

abstract：:The uptake of radioactive biotin has been studied in glial cell cultures from dissociated cerebral hemispheres of newborn rats. We describe saturable kinetics for the biotin uptake at biotin concentrations of less than 60 nM and linear at greater than 60 nM. The uptake appeared temperature sensitive, Na+ independent, ...

journal_title：Journal of neurochemistry

authors： Rodriguez-Pombo P,Ugarte M

更新日期：1992-04-01 00:00:00

abstract：:The interaction of endogenous and exogenous stimulators of innate immunity was examined in primary cultures of mouse microglial cells and macrophages after application of defined Toll-like receptor (TLR) agonists [lipopolysaccharide (LPS) (TLR4), the synthetic lipopeptide Pam3Cys-Ser-Lys4 (Pam3Cys) (TLR2) and single-s...

journal_title：Journal of neurochemistry

authors： Lotz M,Ebert S,Esselmann H,Iliev AI,Prinz M,Wiazewicz N,Wiltfang J,Gerber J,Nau R

更新日期：2005-07-01 00:00:00

abstract：:Unilateral ligation of the left common carotid artery in anesthetized Mongolian gerbils resulted in a steep rise in extracellular dopamine in the ipsilateral striatum in 9 out of 19 animals. Extracellular dopamine was measured by cerebral dialysis in vivo and reached a peak of 0.19 mM at 40 min. At the same time, the ...

journal_title：Journal of neurochemistry

authors： Slivka A,Brannan TS,Weinberger J,Knott PJ,Cohen G

更新日期：1988-06-01 00:00:00

abstract：:Long-chain polyunsaturated (n-3) fatty acids have been reported to influence the efficiency of membrane receptors, transporters and enzymes. Because the brain is particularly rich in docosahexaenoic acid (DHA, 22:6 n-3), the present study addresses the question of whether the 22:6 n-3 fatty acid deficiency induces dis...

journal_title：Journal of neurochemistry

authors： Ximenes da Silva A,Lavialle F,Gendrot G,Guesnet P,Alessandri JM,Lavialle M

更新日期：2002-06-01 00:00:00

abstract：:This Editorial highlights an article in the current issue by Tapia-Rojas and Inestrosa suggesting that attenuation of Wnt signalling may be a triggering factor for the pathogenesis of Alzheimer disease (AD) in the J20 mouse model of AD. Their study utilises Wnt signalling inhibitors that operate at different points in...

journal_title：Journal of neurochemistry

authors： Garner B,Ooi L

更新日期：2018-02-01 00:00:00

abstract：:GD3 synthase (Sial-T2) is a key enzyme of ganglioside synthesis that, in concert with GM2 synthase (GalNAc-T), regulates the ratio of a- and b-pathway gangliosides. In this work, we study the sub-Golgi location of an epitope-tagged version of chicken Sial-T2 transfected to CHO-K1 cells. The expressed protein was enzym...

journal_title：Journal of neurochemistry

authors： Daniotti JL,Martina JA,Giraudo CG,Zurita AR,Maccioni HJ

更新日期：2000-04-01 00:00:00

abstract：:Ketamine is an anesthetic that exhibits analgesic, psychotomimetic, and rapid antidepressant effects that are of particular neuropharmacological interest. Recent studies revealed astrocytic Ca(2+) signaling and regulated exocytosis as ketamine-targeted processes. Thus high-resolution cell-attached membrane capacitance...

journal_title：Journal of neurochemistry

authors： Lasič E,Rituper B,Jorgačevski J,Kreft M,Stenovec M,Zorec R

更新日期：2016-09-01 00:00:00

abstract：:To investigate if the prevention of disulfide bond formation affects the intracellular transport, sorting, and processing of a distinct set of neuroendocrine proteins in the regulated secretory pathway, we have treated Xenopus intermediate pituitaries with the thiol-reducing agent dithiothreitol. Pulse-chase incubatio...

journal_title：Journal of neurochemistry

authors： Van Horssen AM,Van Kuppeveld FJ,Martens GJ

更新日期：1998-07-01 00:00:00

abstract：:Proteolytic processing and phosphorylation of amyloid precursor protein (APP), and hyperphosphorylation of tau protein, have been shown to be increased in Alzheimer's disease (AD) brains, leading to increased production of beta-amyloid (Abeta) peptides and neurofibrillary tangles, respectively. These observations sugg...

journal_title：Journal of neurochemistry

authors： Butterfield DA,Abdul HM,Opii W,Newman SF,Joshi G,Ansari MA,Sultana R

更新日期：2006-09-01 00:00:00

abstract：:PCR was used to isolate identical partial cDNA clones encoding a serotonin 5-HT3 receptor subunit from rat nodose and superior cervical ganglia. The amino acid sequence predicted from these clones, extending from the putative transmembrane domain I to the stop codon, demonstrated a 93% homology with the 5-HT3 receptor...

journal_title：Journal of neurochemistry

authors： Miquel MC,Emerit MB,Gingrich JA,Nosjean A,Hamon M,el Mestikawy S

更新日期：1995-08-01 00:00:00

abstract：:Fused in sarcoma (FUS) is a ubiquitously expressed RNA/DNA-binding protein that plays different roles in the cell. FUS pathology has been reported in neurodegenerative diseases amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Mutations in FUS have also been linked to a subset of familial ALS. FUS...

journal_title：Journal of neurochemistry

authors： Arenas A,Kuang L,Zhang J,Kingren MS,Zhu H

更新日期：2020-12-22 00:00:00

abstract：:We report the distribution of choline acetyltransferase (ChAT) activity in the laminae of the rat olfactory tubercle. Within its posterior medical portion, the tubercle contains three parallel histological laminae that can be separated by cutting tangential sections from frozen tissue. ChAT was measured in homogenates...

journal_title：Journal of neurochemistry

authors： Gordon CR,Krieger NR

更新日期：1983-01-01 00:00:00

abstract：:2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a ubiquitous environmental pollutant that could induce significant toxic effects in the human nervous system. However, the underlying molecular mechanism has not been entirely elucidated. Reactive astrogliosis has implicated in various neurological diseases via the product...

journal_title：Journal of neurochemistry

authors： Zhang Y,Nie X,Tao T,Qian W,Jiang S,Jiang J,Li A,Guo A,Xu G,Wu Q

更新日期：2014-06-01 00:00:00

abstract：:Dendritic trafficking and translation of brain-derived neurotrophic factor (BDNF) transcripts play a key role in mediating synaptic plasticity. Recently, we demonstrated that siRNA-mediated knockdown of translin, an RNA-binding protein, impairs KCl-induced dendritic trafficking of BDNF mRNA in cultured hippocampal neu...

journal_title：Journal of neurochemistry

authors： Wu YC,Williamson R,Li Z,Vicario A,Xu J,Kasai M,Chern Y,Tongiorgi E,Baraban JM

更新日期：2011-03-01 00:00:00

abstract：:Juvenile neuronal ceroid lipofuscinosis (Batten disease) is a neurodegenerative disorder caused by mutation in CLN3. Defective autophagy and concomitant accumulation of autofluorescence enriched with mitochondrial ATP synthase subunit c were previously discovered in Cln3 mutant knock-in mice. In this study, we show th...

journal_title：Journal of neurochemistry

authors： Chang JW,Choi H,Cotman SL,Jung YK

更新日期：2011-02-01 00:00:00

abstract：:Aberrant production of amyloid-beta peptides by processing of the beta-amyloid precursor protein leads to the formation of characteristic extracellular protein deposits which are thought to be the cause of Alzheimer's disease. Therefore, inhibiting the key enzymes responsible for amyloid-beta peptide generation, beta-...

journal_title：Journal of neurochemistry

authors： Beher D,Wrigley JD,Owens AP,Shearman MS

更新日期：2002-08-01 00:00:00

abstract：:Female mice are more susceptible to apolipoprotein E (apoE4)-induced cognitive deficits than male mice. These deficits can be antagonized by stimulating androgen receptors (ARs). To determine the role of AR in the cognitive effects of apoE4, we backcrossed mutant mice with a naturally occurring defect in the AR [testi...

journal_title：Journal of neurochemistry

authors： Rizk-Jackson A,Robertson J,Raber J

更新日期：2008-04-01 00:00:00

abstract：:The content of 1,2-diacylglycerol (DAG) was determined in sciatic nerves from normal and streptozotocin-induced diabetic rats. In nerves frozen in situ, DAG content was reduced 22% in the proximal region and 77% in the distal region of diabetic nerve, principally because of the loss of associated fat. DAG levels in fr...

journal_title：Journal of neurochemistry

authors： Zhu X,Eichberg J

更新日期：1990-09-01 00:00:00

abstract：:It is well-known that the extracellular concentration of calcium affects neuronal excitability and synaptic transmission. Less is known about the physiological concentration of extracellular calcium in the brain. In electrophysiological brain slice experiments, the artificial cerebrospinal fluid traditionally contains...

journal_title：Journal of neurochemistry

authors： Forsberg M,Seth H,Björefeldt A,Lyckenvik T,Andersson M,Wasling P,Zetterberg H,Hanse E

更新日期：2019-05-01 00:00:00

abstract：:Animals treated with high doses of amphetamines have been used as a model of schizophrenia due to the similarities between the psychosis associated with this mental disorder and that induced by chronic amphetamine abuse. When administered to naive rats in high doses, the amphetamine-like CNS stimulant methamphetamine ...

journal_title：Journal of neurochemistry

authors： Schmidt CJ,Sonsalla PK,Hanson GR,Peat MA,Gibb JW

更新日期：1985-03-01 00:00:00

abstract：:Metallothionein (MT) protein and mRNA levels were monitored following exposure of rat neonatal primary astrocyte cultures to methylmercury (MeHg). MT-I and MT-II mRNAs were probed on northern blots with an [alpha-32P]dCTP-labeled synthetic cDNA probe specific for rat MT mRNA. MT-I and MT-II mRNAs were detected in untr...

journal_title：Journal of neurochemistry

authors： Rising L,Vitarella D,Kimelberg HK,Aschner M

更新日期：1995-10-01 00:00:00

abstract：:The two proteins reggie-1/flotillin-2 and reggie-2/flotillin-1 form microdomains at the plasma membrane and at intracellular compartments where src tyrosine kinases associate with them. Specific GPI-anchored proteins, in particular prion protein and Thy-1, co-cluster with reggie microdomains at the plasma membrane and...

journal_title：Journal of neurochemistry

authors： Stuermer CA

更新日期：2011-03-01 00:00:00

abstract：:The blood-brain barrier (BBB) formed by brain microvascular endothelial cells (BMVEC) regulates the passage of molecules and leukocytes in and out of the brain. Oxidative stress is a major underlying cause of neurodegenerative and neuroinflammatory disorders and BBB injury associated with them. Using human BMVEC grown...

journal_title：Journal of neurochemistry

authors： Haorah J,Ramirez SH,Schall K,Smith D,Pandya R,Persidsky Y

更新日期：2007-04-01 00:00:00

abstract：:We recently described a new protein associated exclusively with neuronal clathrin-coated vesicles (CCVs), and characterized two monoclonal antibodies that react with it (S-8G8 and S-6G7). In this report, the association of neuronal protein of 185 kilodaltons (NP185) with CCV kinases and its interaction with tubulin ar...

journal_title：Journal of neurochemistry

authors： Kohtz DS,Puszkin S

更新日期：1989-01-01 00:00:00