Abstract:
:The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, and the hydrolytic activity of the recombinant protein (OPE*) turned out to be improved from a kinetic point of view. In this study, we analyze the effects of sorbitol during the expression of OPE*, at first added as an additional carbon source, and methanol as inducer. The O. piceae enzyme was successfully used for PVAc hydrolysis, suggesting its potential applicability in recycled paper production to decrease stickies problems.
journal_name
Bioengineeredjournal_title
Bioengineeredauthors
Barba Cedillo V,Prieto A,Martínez MJdoi
10.4161/bioe.22818subject
Has Abstractpub_date
2013-07-01 00:00:00pages
249-53issue
4eissn
2165-5979issn
2165-5987pii
22818journal_volume
4pub_type
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