Abstract：
BACKGROUND:The aim of this study was identification of the epidemiology of Prototheca zopfii species from the milk samples of dairy cattle in Isfahan, central Iran. METHODS:Milk samples were obtained from 230 dairy cattle, 130 with and 100 without mastitis, in Isfahan. The samples were cultured in Prototheca Isolation Medium (PIM) and Sabouraud's dextrose agar. All P. zopfii isolates were identified by morphological and biochemical methods. Then, as a confirmatory test they were examined by genotype-specific PCR. RESULTS:Four P. zopfii strains (3.07%) were isolated from the 130 samples of dairy cattle with clinical mastitis and there was no isolation from totally 100 samples of healthy bovines without mastitis. Specific PCR product (about 946 bp) was detected in four isolates. CONCLUSION:It seems that P. zopfii genotype II plays a key role in affecting bovine mastitis that confirmed other previous studies. Our study was the first, which identified the Prototheca species by traditional and molecular methods in Iran and Middle East as well.
journal_name
Iran J Public Healthjournal_title
Iranian journal of public healthauthors
Zaini F,Kanani A,Falahati M,Fateh R,Salimi-Asl M,Saemi N,Farahyar Sh,Kheirabad AK,Nazeri Msubject
Has Abstractpub_date
2012-01-01 00:00:00pages
84-8issue
8eissn
2251-6085issn
2251-6093journal_volume
41pub_type
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