Abstract:
:The small abalone, Haliotis diversicolor supertexta, of the family Haliotidae, is one of the most important species of marine shellfish in eastern Asia. Over the past few decades, this species has drastically declined in Korea. Thus, hatchery-bred seeds have been released into natural coastal areas to compensate for the reduced fishery resources. However, information on the genetic background of the small abalone is scarce. In this study, 20 polymorphic microsatellite DNA markers were identified using next-generation sequencing techniques and used to compare allelic variation between wild and released abalone populations in Korea. Using high-throughput genomic sequencing, a total of 1516 (2.26%; average length of 385 bp) reads containing simple sequence repeats were obtained from 86,011 raw reads. Among the 99 loci screened, 28 amplified successfully, and 20 were polymorphic. When comparing allelic variation between wild and released abalone populations, a total of 243 different alleles were observed, with 18.7 alleles per locus. High genetic diversity (mean heterozygosity = 0.81; mean allelic number = 15.5) was observed in both populations. A statistical analysis of the fixation index (F(ST)) and analysis of molecular variance (AMOVA) indicated limited genetic differences between the two populations (F(ST) = 0.002, p > 0.05). Although no significant reductions in the genetic diversity were found in the released population compared with the wild population (p > 0.05), the genetic diversity parameters revealed that the seeds released for stock abundance had a different genetic composition. These differences are likely a result of hatchery selection and inbreeding. Additionally, all the primer pair sets were effectively amplified in another congeneric species, H. diversicolor diversicolor, indicating that these primers are useful for both abalone species. These microsatellite loci may be valuable for future aquaculture and population genetic studies aimed at developing conservation and management plans for these two abalone species.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
An HS,Lee JW,Hong SWdoi
10.3390/ijms130910750subject
Has Abstractpub_date
2012-01-01 00:00:00pages
10750-64issue
9issn
1422-0067pii
ijms-13-10750journal_volume
13pub_type
杂志文章abstract::Self-assembling peptides have been employed as biotemplates for biomineralization, as the morphologies and sizes of the inorganic materials can be easily controlled. We synthesized two types of highly ordered self-assembling peptides with different secondary structures and investigated the effects of secondary structu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20184650
更新日期:2019-09-19 00:00:00
abstract::The maturation of iPSC-derived cardiomyocytes is still a critical point for their application in cardiovascular research as well as for their clinical use. Although multiple differentiation protocols have been established, researchers failed to generate fully mature cardiomyocytes in vitro possessing identical phenoty...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21082819
更新日期:2020-04-17 00:00:00
abstract::At a resting sarcomere length of approximately 2.2 µm bony fish muscles put into rigor in the presence of BDM (2,3-butanedione monoxime) to reduce rigor tension generation show the normal arrangement of myosin head interactions with actin filaments as monitored by low-angle X-ray diffraction. However, if the muscles a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19072091
更新日期:2018-07-18 00:00:00
abstract::The recognition of a distinct fat depot, the dermal white adipose tissue (dWAT), points out the complexity of the interaction among skin resident cells: keratinocytes, dermal fibroblasts (DFs) and adipocytes in response to physiological (diet, age) and pathological (injury) stimulations. dWAT has been recognized as a ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21238955
更新日期:2020-11-25 00:00:00
abstract::The proper functioning of the endoplasmic reticulum (ER) is crucial for multiple cellular activities and survival. Disturbances in the normal ER functions lead to the accumulation and aggregation of unfolded proteins, which initiates an adaptive response, the unfolded protein response (UPR), in order to regain normal ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17091558
更新日期:2016-09-15 00:00:00
abstract::A detailed understanding of the mechanism of unfolding, aggregation, and associated rheological changes is developed in this study for β-Lactoglobulin at different pH values through concomitant measurements utilizing dynamic light scattering (DLS), optical microrheology, Raman spectroscopy, and differential scanning c...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160817719
更新日期:2015-08-03 00:00:00
abstract::One of the most striking features occurring in the root-knot nematode Meloidogyne incognita induced galls is the reorganization of the vascular tissues. During the interaction of the model tree species Populus and M. incognita, a pronounced xylem proliferation was previously described in mature galls. To better charac...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21020406
更新日期:2020-01-09 00:00:00
abstract::Urinary tract infections (UTIs) and catheter-associated UTIs (CAUTIs) are the principal hospital-acquired infections. Proteus mirabilis is characterized by several virulence factors able to promote adhesion and biofilm formation and ameliorate the colonization of urinary tract and the formation of crystalline biofilms...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18020414
更新日期:2017-02-15 00:00:00
abstract::Chlorogenic (CA) and rosmarinic (RA) acids are two natural bioactive hydroxycinnamic acids whose antioxidant properties can be modulated by the chelation of metal ions. In this work, the interactions of these two carboxylic phenols with calcium ions and the impact of such interactions on their antioxidant activity wer...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21144948
更新日期:2020-07-13 00:00:00
abstract::Normal tissue toxicity is a dose-limiting factor in radiation therapy. Therefore, a detailed understanding of the normal tissue response to radiation is necessary to predict the risk of normal tissue toxicity and to development strategies for tissue protection. One component of normal tissue that is continuously expos...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21072336
更新日期:2020-03-27 00:00:00
abstract::Melatonin exerts its actions through membrane MT1/MT2 melatonin receptors, which belong to the super family of G-protein-coupled receptors consisting of the typical seven transmembrane domains. MT1 and MT2 receptors are expressed in various tissues of the body either as single ones or together. A growing literature su...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms140612458
更新日期:2013-06-13 00:00:00
abstract::Increasing photosynthetic ability as a whole is essential for acquiring higher crop yields. Nonleaf green organs (NLGOs) make important contributions to photosynthate formation, especially under stress conditions. However, there is little information on the pod wall in legume forage related to seed development and yie...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21124457
更新日期:2020-06-23 00:00:00
abstract::Ectophosphatases are surface membrane-bound proteins whose active sites face the extracellular medium. These enzymes have been reported in several microorganisms including a large number of medically relevant fungal species. An effective technique for identifying ectophosphatases is performing phosphatase activity ass...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms15022289
更新日期:2014-02-06 00:00:00
abstract::Chitosan, a naturally occurring polysaccharide with abundant resources, has been extensively exploited for various biomedical applications, typically as wound dressings owing to its unique biocompatibility, good biodegradability and excellent antibacterial properties. In this work, composite nanofibrous membranes of c...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms11093529
更新日期:2010-09-21 00:00:00
abstract::Genomic selection (GS) is a strategy to predict the genetic merits of individuals using genome-wide markers. However, GS prediction accuracy is affected by many factors, including missing rate and minor allele frequency (MAF) of genotypic data, GS models, trait features, etc. In this study, we used one wheat populatio...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041342
更新日期:2020-02-17 00:00:00
abstract::DNA G-quadruplexes (G4s) form in relevant genomic regions and intervene in several biological processes, including the modulation of oncogenes expression, and are potential anticancer drug targets. The human KRAS proto-oncogene promoter region contains guanine-rich sequences able to fold into G4 structures. Here, by u...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010448
更新日期:2021-01-05 00:00:00
abstract::Riboflavin is the biological precursor of two important flavin cofactors-flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN)-that are critical prosthetic groups in several redox enzymes. While dietary supplementation with riboflavin is a recognized support therapy in several inborn errors of metabolism, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21197063
更新日期:2020-09-25 00:00:00
abstract::Lung squamous cell carcinoma (LUSC) has a poor prognosis, in part due to poor therapeutic response and limited therapeutic alternatives. Lichens are symbiotic organisms, producing a variety of substances with multiple biological activities. (+)-Usnic acid, an important biologically active metabolite of lichens, has be...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21030876
更新日期:2020-01-29 00:00:00
abstract::RNA binding protein (RBPs) and microRNAs (miRNAs or miRs) are post-transcriptional regulators of gene expression that are implicated in development of cancers. Although their individual roles have been studied, the crosstalk between RBPs and miRNAs is under intense investigation. Here, we show that in breast cancer ce...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16047112
更新日期:2015-03-30 00:00:00
abstract::High dietary salt intake has been listed among the top ten risk factors for disability-adjusted life years. We discuss the role of endogenous cardiotonic steroids in mediating the dietary salt-induced hypertension and organ damage. ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20030590
更新日期:2019-01-30 00:00:00
abstract::The glutamatergic system is a key point in pathogenesis of schizophrenia. Sarcosine (N-methylglycine) is an exogenous amino acid that acts as a glycine transporter inhibitor. It modulates glutamatergic transmission by increasing glycine concentration around NMDA (N-methyl-d-aspartate) receptors. In patients with schiz...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,随机对照试验
doi:10.3390/ijms161024475
更新日期:2015-10-15 00:00:00
abstract::Stearoyl-CoA desaturase 1 (SCD1), an enzyme that is involved in the biosynthesis of monounsaturated fatty acids, induces the reprogramming of cardiomyocyte metabolism. Thyroid hormones (THs) activate both lipolysis and lipogenesis. Many genes that are involved in lipid metabolism, including Scd1, are regulated by THs....
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010109
更新日期:2020-12-24 00:00:00
abstract::Streptococcus mutans (S. mutans) is widely regarded as the main cause of human dental caries via three main virulence factors: adhesion, acidogenicity, and aciduricity. Citric acid is one of the antibiotic agents that can inhibit the virulence capabilities of S. mutans. A full understanding of the acidic resistance me...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249481
更新日期:2020-12-13 00:00:00
abstract::Fused deposit modeling (FDM) 3D printing technology cannot generate scaffolds with high porosity while maintaining good integrity, anatomical-surface detail, or high surface area-to-volume ratio (S/V). Solvent casting and particulate leaching (SCPL) technique generates scaffolds with high porosity and high S/V. Howeve...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21010315
更新日期:2020-01-02 00:00:00
abstract::It is now established that adipose tissue, skeletal muscle, and heart are endocrine organs and secrete in normal and in pathological conditions several molecules, called, respectively, adipokines, myokines, and cardiokines. These secretory proteins constitute a closed network that plays a crucial role in obesity and a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21218372
更新日期:2020-11-08 00:00:00
abstract::Studies have shown that the type II pyridoxal phosphate-dependent decarboxylase (PLP_deC) genes produce secondary metabolites and flavor volatiles in plants, and TDC (tryptophan decarboxylase), a member of the PLP_deC family, plays an important role in the biosynthesis of terpenoid indole alkaloids (TIAs). In this stu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21010054
更新日期:2019-12-20 00:00:00
abstract::Preimplantation embryo manipulations during standard assisted reproductive technologies (ART) have significant repercussions on offspring. However, few studies to date have investigated the potential long-term outcomes associated with the vitrification procedure. Here, we performed an experiment to unravel the particu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21228642
更新日期:2020-11-16 00:00:00
abstract::Quantification of gene expression is crucial to connect genome sequences with phenotypic and physiological data. RNA-Sequencing (RNA-Seq) has taken a prominent role in the study of transcriptomic reactions of plants to various environmental and genetic perturbations. However, comparative tests of different tools for R...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051720
更新日期:2020-03-03 00:00:00
abstract::Class I and II histone deacetylases (HDAC) are considered important regulators of immunity and inflammation. Modulation of HDAC expression and activity is associated with altered inflammatory responses but reports are controversial and the specific impact of single HDACs is not clear. We examined class I and II HDACs ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21238943
更新日期:2020-11-25 00:00:00
abstract::The order Nymphaeales, consisting of three families with a record of eight genera, has gained significant interest from botanists, probably due to its position as a basal angiosperm. The phylogenetic relationships within the order have been well studied; however, a few controversial nodes still remain in the Nymphaeac...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19123780
更新日期:2018-11-28 00:00:00