当前位置： SCI文献检索 > JOURNAL OF INSECT PHYSIOLOGY期刊下所有文献 > Midgut proteins released by microapocrine secretion in Spodoptera frugiperda.

Midgut proteins released by microapocrine secretion in Spodoptera frugiperda.

Abstract：

:Microapocrine vesicles bud from the lepidopteran midgut microvilli as double membrane vesicles. To identify the proteins secreted by this process, antibodies raised against isolated microapocrine vesicles from Spodoptera frugiperda were used for screening a midgut cDNA expression library. Positive clones were sequenced, assembled and N blasted against S. frugiperda sequences obtained by pyrosequencing midgut mRNA. This procedure led to the extension of microapocrine sequences that were annotated. A similar procedure was used to identify midgut microvillar proteins that necessarily are part of the microapocrine vesicle. Forty-eight proteins were associated with microvillar membranes. They pertain to 8 functional groups: digestive enzymes, peritrophic membrane, protection, transporters, receptors, secretory machinery, cytoskeleton and signaling, and unknown. Twenty-eight proteins are putatively secreted by microapocrine secretion. Most of them are digestive enzymes, but the list also includes proteins involved in protection and in peritrophic membrane formation. Among the identified digestive enzymes, aminopeptidases are typically microvillar and group into the classes 1, 2, 3, 5, and 6. There are two amylases secreted by microapocrine secretion: one is a digestive enzyme and the other is a transporter-like amylase with no clear function. One lipase has a predicted transmembrane loop, whereas the others are supposed to be secreted by microapocrine secretion and be digestive. Trypsin is membrane bound and is delivered by microapocrine secretion, but has no predicted features to bind membranes. It may remain bound through the signal peptide till be delivered into the midgut lumen. Proteins supposed to be involved in the microapocrine secretory machinery were: calmodulin, annexin, myosin 7a, and gelsolin 1. Their putative roles are discussed, but more research is necessary to settle this subject.

journal_name

J Insect Physiol

journal_title

Journal of insect physiology

authors

Silva W,Cardoso C,Ribeiro AF,Terra WR,Ferreira C

doi

10.1016/j.jinsphys.2012.10.015

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

70-80

issue

eissn

0022-1910

issn

1879-1611

pii

S0022-1910(12)00267-3

journal_volume

pub_type

杂志文章

在线工具

Midgut proteins released by microapocrine secretion in Spodoptera frugiperda.