Abstract:
:Microspheres of bovine serum albumin (BSA) and silk fibroin are produced by applying ultrasound in a biphasic system consisting of an aqueous protein solution and an organic solvent. The protein microspheres are dispersed in an aqueous media where the protein remains at the interface covering the organic solvent. This only occurs when high shear forces are applied that induce changes to force the protein to the interface. Fourier transform infrared results indicate a large increase in the content of the β-sheet during the formation of silk fibroin microspheres. Molecular dynamics simulations show a clear adaption on the 3D structure of BSA when stabilized at the interface, without major changes in secondary structure. Further studies demonstrate that high water content, oil solvents, and larger peptides with separated and clear hydrophobic and hydrophilic areas lead to more stable and smaller spheres. This is the first time that these results are presented. We also present herein the rationale to produce tailored protein microspheres with a controlled size, controlled charge, and increased stability.
journal_name
Mol Pharmjournal_title
Molecular pharmaceuticsauthors
Silva R,Ferreira H,Azoia NG,Shimanovich U,Freddi G,Gedanken A,Cavaco-Paulo Adoi
10.1021/mp3001827subject
Has Abstractpub_date
2012-11-05 00:00:00pages
3079-88issue
11eissn
1543-8384issn
1543-8392journal_volume
9pub_type
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