Abstract:
:Small molecules are widely used in chemical biology without complete knowledge of their target profile, at risk of deriving conclusions that ignore potential confounding effects from unknown off-target interactions. The prediction and further experimental confirmation of novel affinities for PJ34 on Pim1 (IC(50) = 3.7 μM) and Pim2 (IC(50) = 16 μM) serine/threonine kinases, together with their involvement in many of the processes relevant to PARP biology, questions the appropriateness of using PJ34 as a chemical tool to probe the biological role of PARP1 and PARP2 at the high micromolar concentrations applied in most studies.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Antolín AA,Jalencas X,Yélamos J,Mestres Jdoi
10.1021/cb300317ysubject
Has Abstractpub_date
2012-12-21 00:00:00pages
1962-7issue
12eissn
1554-8929issn
1554-8937journal_volume
7pub_type
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