Abstract：
:The present study was designed to evaluate the effects of chronic fluorosis on the dynamics (including fusion and fission proteins), fragmentation, and distribution of mitochondria in the cortical neurons of the rat brain in an attempt to elucidate molecular mechanisms underlying the brain damage associated with excess accumulation of fluoride. Sixty Sprague-Dawley rats were divided randomly into three groups of 20 each, that is, the untreated control group (drinking water naturally containing <0.5 mg fluoride/l, NaF), the low-fluoride group (whose drinking water was supplemented with 10 mg fluoride/l) and the high-fluoride group (50 mg fluoride/l). After 6 months of exposure, the expression of mitofusin-1 (Mfn1), fission-1 (Fis1), and dynamin-related protein-1 (Drp1) at both the protein and mRNA levels were detected by Western blotting, immunohistochemistry, and real-time PCR, respectively. Moreover, mitochondrial morphology and distribution in neurons were observed by transmission electron or fluorescence microscopy. In the cortices of the brains of rats with chronic fluorosis, the level of Mfn1 protein was clearly reduced, whereas the levels of Fis1 and Drp1 were elevated. The alternations of expression of the mRNAs encoding all three of these proteins were almost the same as the corresponding changes at the protein levels. The mitochondria were fragmented and the redistributed away from the axons of the cortical neurons. These findings indicate that chronic fluorosis induces abnormal mitochondrial dynamics, which might in turn result in a high level of oxidative stress.
journal_name
Arch Toxicoljournal_title
Archives of toxicologyauthors
Lou DD,Guan ZZ,Liu YJ,Liu YF,Zhang KL,Pan JG,Pei JJdoi
10.1007/s00204-012-0942-zsubject
Has Abstractpub_date
2013-03-01 00:00:00pages
449-57issue
3eissn
0340-5761issn
1432-0738journal_volume
87pub_type
杂志文章abstract：:The in ovo carcinogenicity assessing (IOCA) assay was used to examine the morphological changes in fetal turkey livers caused by the DNA-reactive carcinogen diethylnitrosamine (DEN). Fertilized turkey eggs were allocated into 3 groups: nondosed control (NDC), vehicle (water) control (VC) and DEN-dosed. At day 0, the f...
journal_title：Archives of toxicology
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journal_title：Archives of toxicology
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journal_title：Archives of toxicology
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1007/BF01270911
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更新日期：2018-07-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-013-1107-4
更新日期：2013-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00204-005-0660-x
更新日期：2005-09-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-003-0460-0
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-016-1829-1
更新日期：2017-04-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-003-0523-2
更新日期：2004-03-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00353357
更新日期：1976-12-23 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
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更新日期：2001-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s002040050368
更新日期：1997-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1987-01-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章,评审
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更新日期：2016-10-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF01973715
更新日期：1991-01-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章,评审
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更新日期：2018-08-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
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更新日期：2004-06-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
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更新日期：2006-03-01 00:00:00
abstract：:Since tattoos became overwhelmingly fashionable worldwide, the demand for removal has proportionally increased, Nd:YAG Q-switch laser being the most commonly used tool for the purpose. In this framework we investigated the composition and products of laser treatment of green tattoo ink, the Green Concentrate from Eter...
journal_title：Archives of toxicology
pub_type： 杂志文章
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更新日期：2020-07-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
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更新日期：2003-02-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s002040050402
更新日期：1997-01-01 00:00:00
abstract：:Pregnant mice administered per os during the days 1--3 (plug day = day 0) post conception (p.c.) with 375 mg 2,2'-dichlorobiphenyl/kilograms/day revealed on day 18 p.c. increased number of resorptions and doses up from 500 milligrams/kilograms/day led to a substantial retardation of the prenatal development. Based on ...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00310329
更新日期：1978-08-09 00:00:00
