Abstract:
:The objective of this study was to phylogenetically analyze microorganisms from the domains Bacteria and Archaea in hypersaline sediment from Death Valley National Park. Using domain-specific primers, a region of the 16S rRNA gene was amplified using polymerase chain reaction (PCR), and the product was subsequently used to create a clone library. A total of 243 bacterial clones, 99 archaeal clones, and 209 bacterial isolates were examined. The 243 clones from Bacteria were affiliated with the following groups: the Bacilli (59 clones) and Clostridia (1) of the Firmicutes, Bacteroidetes (90), Proteobacteria (27), Cyanobacteria (18), Gemmatimonadetes (41), candidate division OP1 (5), Actinobacteria (1), and the Deinococcus-Thermus division (1). Within the class Bacilli, 46 of 59 clones were tentatively identified as 10 unclassified species. The majority of bacterial isolates (130 of 209) were more closely related to the Bacillus subtilis-B. licheniformis clade than to any other recognized taxon, and an Ecotype Simulation analysis of B. subtilis relatives identified four previously unknown ecotypes. Several new genera were discovered within the Bacteroidetes (4) and the Gemmatimonadetes (2). Of the 99 archaeal clones, 94 were tentatively identified as belonging to 3 new genera within the Halobacteriaceae; other clones represented novel species within each of 4 established genera.
journal_name
Microbiologyopenjournal_title
MicrobiologyOpenauthors
Kim JS,Makama M,Petito J,Park NH,Cohan FM,Dungan RSdoi
10.1002/mbo3.20subject
Has Abstractpub_date
2012-06-01 00:00:00pages
135-48issue
2issn
2045-8827journal_volume
1pub_type
杂志文章相关文献
MicrobiologyOpen文献大全abstract::In Escherichia coli, coordinated activation and deactivation of DnaA allows for proper timing of the initiation of chromosomal synthesis at the origin of replication (oriC) and assures initiation occurs once per cell cycle. In vitro, acidic phospholipids reactivate DnaA, and in vivo depletion of acidic phospholipids, ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.46
更新日期:2012-12-01 00:00:00
abstract::By taking advantage of the ballistoconidium-forming capabilities of members of the genus Sporobolomyces, we recovered ten isolates from deciduous tree leaves collected from Vermont and Washington, USA. Analysis of the small subunit ribosomal RNA gene and the D1/D2 domain of the large subunit ribosomal RNA gene indicat...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.374
更新日期:2016-10-01 00:00:00
abstract::Urinary tract infections (UTIs) are among the most common bacterial infections in humans. Although a number of bacteria can cause UTIs, most cases are due to infection by uropathogenic Escherichia coli (UPEC). UPEC are a genetically heterogeneous group that exhibit several virulence factors associated with colonizatio...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.756
更新日期:2019-06-01 00:00:00
abstract::Marine picocyanobacteria belonging to genera Synechococcus and Prochlorococcus are genetically diverged and distributed into distinct biogeographical patterns, and both are infected by genetically closely related cyanopodoviruses. Previous studies have not fully explored whether the two virus-host systems share simila...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1150
更新日期:2020-12-30 00:00:00
abstract::Sustained monoculture often leads to the inhibition of plant growth, the decrease of the soil microbial diversity, and changes in soil microbial community composition, particularly to the accumulation of soil-borne pathogens. In this study, we conducted field experiments to investigate the practical effects of tilling...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1045
更新日期:2020-07-01 00:00:00
abstract::Trying to widen the discussion on the risks associated with dental waste, this study proposed to investigate and genetically compare yeast isolates recovered from dental solid waste and waste workers. Three samples were collected from workers' hands, nasal mucosa, and professional clothing (days 0, 30, and 180), and t...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.554
更新日期:2018-04-01 00:00:00
abstract::This study compared the sensitivity of spores from virulent and attenuated Bacillus anthracis strains in suspension to inactivation by various chemical disinfectants. Spore suspensions from two virulent strains (A0256 and A0372) and two attenuated strains (Sterne and A0141) of B. anthracis were tested against two alde...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.45
更新日期:2012-12-01 00:00:00
abstract::The intraspecies genomic diversity of the single-strand RNA (+) virus species hepatitis A virus (Hepatovirus), hepatitis C virus (Hepacivirus), and hepatitis E virus (Orthohepevirus) was compared. These viral species all can cause liver inflammation (hepatitis), but share no gene similarity. The codon usage of human h...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.973
更新日期:2020-02-01 00:00:00
abstract::Basic cosmetics was used by volunteers belonging to high (HHG) and low (LHG) hydration groups for 4 weeks, and bacterial communities and biophysical parameters in facial skin were analyzed. Hydration level increases and transepidermal water loss and roughness decreases were observed in both groups after cosmetic use. ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.557
更新日期:2018-04-01 00:00:00
abstract::Avian pathogenic Escherichia coli (APEC) causes airsacculitis, polyserositis, septicemia, and other mainly extraintestinal diseases in chickens, ducks, geese, pigeons, and other avian species, and is responsible for great economic losses in the avian industry. The autoinducer 2 (AI-2) quorum sensing system is widely p...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.582
更新日期:2018-08-01 00:00:00
abstract::Resistance to colistin, mediated by chromosomal mutations and more recently, by plasmid-borne mcr genes, is increasingly being reported in bacterial isolates taken from humans, animals, farms, foods, and the environment. To easily identify and contain this quickly spreading menace, efficient diagnostics that are cheap...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.682
更新日期:2019-04-01 00:00:00
abstract::[NiFe]-hydrogenases catalyze the reversible conversion of molecular hydrogen into protons end electrons. This reaction takes place at a NiFe(CN)2 (CO) cofactor located in the large subunit of the bipartite hydrogenase module. The corresponding apo-protein carries usually a C-terminal extension that is cleaved off by a...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1029
更新日期:2020-06-01 00:00:00
abstract::Gene expression in Haloarcula hispanica cells infected with the gammapleolipovirus His2 was studied using a custom DNA microarray. Total RNA from cells sampled at 0, 1, 2, 3, and 4.5 hr postinfection was reverse-transcribed into labeled cDNA and hybridized to microarrays, revealing temporal and differential expression...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1016
更新日期:2020-05-01 00:00:00
abstract::Aeromonas caviae is motile via a polar flagellum in liquid culture, with a lateral flagella system used for swarming on solid surfaces. The polar flagellum also has a role in cellular adherence and biofilm formation. The two subunits of the polar flagellum, FlaA and FlaB, are posttranslationally modified by O-linked g...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.233
更新日期:2015-04-01 00:00:00
abstract::Directed enzyme prodrug therapy (DEPT) is a cancer chemotherapy strategy in which bacterial enzymes are delivered to a cancer site before prodrug administration, resulting in prodrug activation at the cancer site and more localized treatment. A major limitation to DEPT is the poor effectiveness of the most studied enz...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1110
更新日期:2020-10-01 00:00:00
abstract::Phellinus noxius is a pathogenic fungus that causes brown root rot disease, resulting in a widespread tree and crop mortality in the tropics and subtropics. Early stages of this disease are largely asymptomatic, hindering early diagnosis and effective treatment. We hypothesized that P. noxius infection would alter the...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1115
更新日期:2020-10-01 00:00:00
abstract::In our previous work, we demonstrated that viable but nonculturable (VBNC) Vibrio cholerae O1 and O139 were converted to culturable by coculture with eukaryotic cells. Furthermore, we isolated a factor converting VBNC V. cholerae to culturable (FCVC) from a eukaryotic cell line, HT-29. In this study, we purified FCVC ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.264
更新日期:2015-08-01 00:00:00
abstract::Pseudomonas aeruginosa and Staphylococcus aureus mixed-species biofilm infections are more resilient to biocide attacks compared to their single-species counterparts. Therefore, this study used an in vitro model recapitulating bacterial burdens seen in in vivo infections to investigate the interactions of P. aeruginos...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.427
更新日期:2017-06-01 00:00:00
abstract::Bacterial flagellar motility is a complex cellular behavior required for the colonization of the light-emitting organ of the Hawaiian bobtail squid, Euprymna scolopes, by the beneficial bioluminescent symbiont Vibrio fischeri. We characterized the basis of this behavior by performing (i) a forward genetic screen to id...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.96
更新日期:2013-08-01 00:00:00
abstract::The interactions of pathogens and phagocytes are complex. Our study demonstrated that Aeromonas hydrophila B11 can survive in the macrophagocytes of Tilapia mossambica. To explore the regulatory processes of A. hydrophila survival in the macrophagocytes, we used the mini-Tn10 transposon mutagenesis system to build a m...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.468
更新日期:2017-08-01 00:00:00
abstract::In rod-shaped bacteria, the proper placement of the division septum at the midcell relies, at least partially, on the proteins of the Min system as an inhibitor of cell division. The main principle of Min system function involves the formation of an inhibitor gradient along the cell axis; however, the establishment of...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.337
更新日期:2016-06-01 00:00:00
abstract::Microbial biofilms are communities of surface-adhered cells enclosed in a matrix of extracellular polymeric substances. Bacterial cells in biofilm are 10~1,000-fold more resistant to antimicrobials than the planktonic cells. Burgeoning antibiotic resistance in Pseudomonas aeruginosa biofilm has necessitated the develo...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.545
更新日期:2018-02-01 00:00:00
abstract::In microbes that fix nitrogen, nitrogenase catalyzes the conversion of N2 to ammonia in an ATP-demanding reaction. To help conserve energy some bacteria inhibit nitrogenase activity upon exposure to ammonium. The purple nonsulfur phototrophic bacterium Rhodopseudomonas palustris strain CGA009 can synthesize three func...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.265
更新日期:2015-08-01 00:00:00
abstract::Copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803 comprises two operons, copMRS and copBAC, which are expressed in response to copper in the media. copBAC codes for a heavy-metal efflux-resistance nodulation and division (HME-RND) system, while copMRS codes for a protein of unknown function, Co...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.231
更新日期:2015-02-01 00:00:00
abstract::In cyanobacteria, genes conferring mercury resistance are not organized as mer-operon, unlike in other bacterial phyla. Synechocystis contains only a putative MerR regulator, Slr0701, and a mercury reductase, MerA, located aside from each other in the genome. The slr0701-mutant showed reduction in photosynthetic activ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.838
更新日期:2019-09-01 00:00:00
abstract::To investigate the possible effects of sulfamonomethoxine (SMM) on Nile tilapia (Oreochromis niloticus), we quantitatively evaluated the microbial shifts in the intestines of Nile tilapia in response to different doses of SMM (200 and 300 mg/kg) using 16S rRNA gene sequencing. At the phylum level, the control group (0...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1116
更新日期:2020-11-01 00:00:00
abstract::Nuclease colicins bind their target receptor BtuB in the outer membrane of sensitive Escherichia coli cells in the form of a high-affinity complex with their cognate immunity proteins. The release of the immunity protein from the colicin complex is a prerequisite for cell entry of the colicin and occurs via a process ...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.122
更新日期:2013-10-01 00:00:00
abstract::The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals. Little information has been reported on these aspects of the goat GIT. This s...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.820
更新日期:2019-09-01 00:00:00
abstract::The black pigmentation-related genes in Porphyromonas gingivalis are primarily involved in regulating gingipain functions. In this study, we identified a pigmentation-related gene, designated as pgn_0361. To characterize the role of pgn_0361 in regulating P. gingivalis-mediated epithelial cell detachment and inhibitio...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1128
更新日期:2020-12-01 00:00:00
abstract::Methicillin-resistant S. aureus has been reported as the main pathogen involved in chronic infections, osteomyelitis, and prosthetic joint infections. The host/pathogen interaction is dynamic and requires several changes to promote bacterial survival. Here, we focused on the internalization and persistence behavior of...
journal_title:MicrobiologyOpen
pub_type: 杂志文章
doi:10.1002/mbo3.1017
更新日期:2020-05-01 00:00:00