Abstract:
:Porphyromonas gingivalis is a major etiological agent in chronic and aggressive forms of periodontal disease. The organism is an asaccharolytic anaerobe and is a constituent of mixed species biofilms in a variety of microenvironments in the oral cavity. P. gingivalis expresses a range of virulence factors over which it exerts tight control. High-throughput sequencing technologies provide the opportunity to relate functional genomics to basic biology. In this study we report qualitative and quantitative RNA-Seq analysis of the transcriptome of P. gingivalis. We have also applied RNA-Seq to the transcriptome of a ΔluxS mutant of P. gingivalis deficient in AI-2-mediated bacterial communication. The transcriptome analysis confirmed the expression of all predicted ORFs for strain ATCC 33277, including 854 hypothetical proteins, and allowed the identification of hitherto unknown transcriptional units. Twelve non-coding RNAs were identified, including 11 small RNAs and one cobalamin riboswitch. Fifty-seven genes were differentially regulated in the LuxS mutant. Addition of exogenous synthetic 4,5-dihydroxy-2,3-pentanedione (DPD, AI-2 precursor) to the ΔluxS mutant culture complemented expression of a subset of genes, indicating that LuxS is involved in both AI-2 signaling and non-signaling dependent systems in P. gingivalis. This work provides an important dataset for future study of P. gingivalis pathophysiology and further defines the LuxS regulon in this oral pathogen.
journal_name
Front Cell Infect Microbioljournal_title
Frontiers in cellular and infection microbiologyauthors
Hirano T,Beck DA,Demuth DR,Hackett M,Lamont RJdoi
10.3389/fcimb.2012.00079subject
Has Abstractpub_date
2012-06-12 00:00:00pages
79issn
2235-2988journal_volume
2pub_type
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pub_type: 杂志文章
doi:10.3389/fcimb.2018.00363
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abstract::Mycoplasma hyopneumoniae (M. hyopneumoniae) is the causative agent of pandemic pneumonia among pigs, namely, swine enzootic pneumonia. Although M. hyopneumoniae was first identified in 1965, little is known regarding its metabolic pathways, which might play a pivotal role during disease pathogenesis. Lipoate is an ess...
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abstract::Solid-state fermentation of feedstuffs by Bacillus subtilis MA139 can reduce insoluble dietary fiber content in vitro and improve growth performance in pigs. This study was conducted to investigate the effects of dietary corn bran (CB) fermented by B. subtilis on growth performance and gut microbiota composition in fi...
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2012.00162
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abstract::Due to increased travel, climatic, and environmental changes, the incidence of tick-borne disease in both humans and animals is increasing throughout Europe. Therefore, extended surveillance tools are desirable. To accurately screen tick-borne pathogens (TBPs), a large scale epidemiological study was conducted on 7050...
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pub_type: 杂志文章
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abstract::Several bacterial pathogens have TIR domain-containing proteins that contribute to their pathogenesis. We identified a second TIR-containing protein in Brucella spp. that we have designated BtpB. We show it is a potent inhibitor of TLR signaling, probably via MyD88. BtpB is a novel Brucella effector that is translocat...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2013.00028
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abstract::Lipids are essential players in parasites pathogenesis. In particular, the highly phagocytic trophozoites of Entamoeba histolytica, the causative agent of amoebiasis, exhibit a dynamic membrane fusion and fission, in which lipids strongly participate; particularly during the overstated motility of the parasite to reac...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
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abstract:Background:Disseminated histoplasmosis remains a major killer of immunocompromised patients in Latin America. Cytological and histological methods are usually present in most hospitals and may represent a precious diagnostic method. We report 15 years of experience of the department of pathology of the Centre Hospitali...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.591974
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abstract::Vibrio parahaemolyticus elicits gastroenteritis by deploying Type III Secretion Systems (TTSS) to deliver effector proteins into epithelial cells of the human intestinal tract. The bacteria must adhere to the human cells to allow colonization and operation of the TTSS translocation apparatus bridging the bacterium and...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2013.00114
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abstract::An attenuated vaccinia virus-MVTTEAB-was constructed by deletion of non-essential gene segments related to the immunomodulatory and virulence functions of the vaccinia virus Tiantan strain (VVTT). The shuttle plasmids pTC-EGFP, pTE-EGFP, pTA35-EGFP, pTB-EGFP, and pTA66-EGFP were constructed and combined with the early...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00462
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abstract::To define the bottlenecks that restrict antigen expression after oral administration of viral-vectored vaccines, we tracked vectors derived from the human adenovirus type 5 at whole body, tissue, and cellular scales throughout the digestive tract in a murine model of oral delivery. After intragastric administration of...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00006
更新日期:2018-01-25 00:00:00
abstract::Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 is an enteric pathogen that causes life-threatening disease in humans, with cattle being major natural reservoirs. A group of STEC O157:H7 with a dramatic combination of high virulence potentials and super-shedder bovine origin have been isolated. Here, an ST...
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doi:10.3389/fcimb.2020.00271
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abstract::The mechanism by which microRNAs (miRNAs) modulate innate immunity and autophagy has not been fully elucidated in Mycobacterium bovis (M. bovis) infections. In this study, we identified that miR-199a inhibited key innate immune responses and autophagy in murine macrophages infected with M. bovis. Using ex vivo and in ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00238
更新日期:2018-07-10 00:00:00
abstract::An important component in host resistance to malaria infection are inherited mutations that give rise to abnormalities and deficiencies in erythrocyte proteins and enzymes. Understanding how such mutations confer protection against the disease may be useful for developing new treatment strategies. A mouse ENU-induced ...
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pub_type: 杂志文章
doi:10.3389/fcimb.2020.00464
更新日期:2020-09-03 00:00:00
abstract::Francisella tularensis is the causative agent of tularemia and a Tier I bioterrorism agent. In the 1900s, several vaccines were developed against tularemia including the killed "Foshay" vaccine, subunit vaccines comprising F. tularensis protein(s) or lipoproteins(s) in an adjuvant formulation, and the F. tularensis Li...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2018.00154
更新日期:2018-05-15 00:00:00
abstract::The NLRP3 inflammasome and IL-1β release have recently been suggested to be important for the progression of urinary tract infection (UTI). However, much is still unknown regarding the interaction of UPEC and the NLRP3 inflammasome. The purpose of this study was to elucidate what virulence factors uropathogenic Escher...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00081
更新日期:2018-03-14 00:00:00
abstract::Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne's disease, a chronic granulomatous enteritis in ruminants. Furthermore, infections of humans with MAP have been reported and a possible association with Crohn's disease and diabetes type I is currently discussed. MAP owns large sequence polymorphisms (...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2014.00110
更新日期:2014-08-14 00:00:00
abstract::The natural history of genital Chlamydia trachomatis infections can vary widely; infections can spontaneously resolve but can also last from months to years, potentially progressing to cause significant pathology. The host and bacterial factors underlying this wide variation are not completely understood, but emphasiz...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2014.00072
更新日期:2014-06-03 00:00:00
abstract::Background: Intraocular tuberculosis (IOTB), an extrapulmonary manifestation of tuberculosis of the eye, has unique and varied clinical presentations with poorly understood pathogenesis. As it is a significant cause of inflammation and visual morbidity, particularly in TB endemic countries, it is essential to study th...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
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abstract::Pneumocystis pneumonia (PCP) remains the most frequent AIDS-defining illness in developed countries. This infection also occurs in non-AIDS immunosuppressed patients, e.g., those who have undergone an organ transplantation. Moreover, mild Pneumocystis jirovecii infections related to low pulmonary fungal burden, freque...
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pub_type: 杂志文章,评审
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abstract::Genital tract infections with Chlamydia trachomatis (C. trachomatis) are the most frequent sexually transmitted disease worldwide. Severe clinical sequelae such as pelvic inflammatory disease (PID), tubal occlusion, and tubal infertility are linked to inflammatory processes of chronically infected tissues. The oxygen ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2014.00043
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
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abstract::Systemic and endemic mycoses are considered life-threatening respiratory diseases which are caused by a group of dimorphic fungal pathogens belonging to the genera Histoplasma, Coccidioides, Blastomyces, Paracoccidioides, Talaromyces, and the newly described pathogen Emergomyces. T-cell mediated immunity, mainly T hel...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
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abstract::Magnaporthe oryzae (Mo) is a model pathogen causing rice blast resulting in yield and economic losses world-wide. CK2 is a constitutively active, serine/threonine kinase in eukaryotes, having a wide array of known substrates, and involved in many cellular processes. We investigated the localization and role of MoCK2 d...
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doi:10.3389/fcimb.2019.00113
更新日期:2019-04-17 00:00:00
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pub_type: 杂志文章
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abstract::Cold shock-domain family proteins (Csps) are highly conserved nucleic acid binding proteins regulating the expression of various genes including those involved in stress resistance and virulence in bacteria. We show here that Csps are involved in virulence, cell aggregation and flagella-based extracellular motility of...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00453
更新日期:2017-10-26 00:00:00
abstract::Additional research on soft ticks in the family Argasidae is needed to bridge the knowledge gap relative to hard ticks of the family Ixodidae; especially, the molecular mechanisms of Ornithodoros biology. Ornithodoros species are vectors of human and animal pathogens that include tick-borne relapsing fever spirochetes...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00152
更新日期:2020-05-05 00:00:00
abstract::Emerging antibiotic resistance in pathogenic bacteria like Mycobacterium sp., poses a threat to human health and therefore calls for the development of novel antibacterial strategies. We have recently discovered that bacterial membrane peptides, such as KdpF, possess anti-virulence properties when overproduced in path...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00115
更新日期:2017-04-06 00:00:00
abstract::Proton pump inhibitors (PPIs) are the standard therapy for gastroesophageal reflux disease. In adults, PPI treatment is associated with Clostridium difficile infections (CDI). In contrast to adults the microbiome of infants develops from sterility at birth toward an adult-like profile in the first years of life. The e...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 临床试验,杂志文章
doi:10.3389/fcimb.2017.00444
更新日期:2017-10-11 00:00:00
abstract::Nocardia is one of the causing agents of bovine mastitis and increasing prevalence of nocardial mastitis in shape of serious outbreaks has been reported from many countries. However, the mechanisms by which this pathogen damages the bovine mammary epithelial cells (bMECs) is not yet studied. Therefore, this study was ...
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pub_type: 杂志文章
doi:10.3389/fcimb.2017.00194
更新日期:2017-05-18 00:00:00