The role of type III secretion system and lens material on adhesion of Pseudomonas aeruginosa to contact lenses.

Abstract:

PURPOSE:To determine the distribution of invasive and cytotoxic genotypes among ocular isolates of P. aeruginosa and investigate the influence of the type III secretion system (T3SS) on adhesion to conventional, cosmetic, and silicone hydrogel contact lenses (CL). METHODS:Clinical isolates from 2001 to 2010 were analyzed by multiplex PCR for exoS, exoU, and exoT genes. Bacterial adhesion to etafilcon, nelfilcon (gray colored), balafilcon, and galyfilcon CL with or without artificial tear fluid (ATF) incubation were compared. Surface characteristics were determined with scanning electron microscopy (SEM). RESULTS:Among 87 total isolates, 64 strains were from microbial keratitis cases. CL-related microbial keratitis (CLMK) isolates were mostly of the cytotoxic genotype (expressing exoU) (P = 0.002). No significant differences were found in bacterial adhesion to all types of CL between the genotypes under T3SS-inducing conditions. A trend for least bacterial adhesion of galyfilcon compared to the other CL was noted for both genotypes. Needle complex pscC mutants adhered less to all materials than the wild type (P < 0.05), indicating a role of the T3SS in contact lens adhesion. ATF-incubated CL had significantly more bacterial adhesion (P < 0.05). SEM showed most of the bacteria adhering on CL surfaces. CONCLUSIONS:CLMK isolates were mostly of cytotoxic genotype. Different genotypes did not significantly differ in its adhesion to various CL. T3SS and other adhesins are involved in bacteria-contact lens adhesion through complex interactions. Contact lens materials may also play an important role in the adherence of both genotypes of P. aeruginosa.

authors

Shen EP,Tsay RY,Chia JS,Wu S,Lee JW,Hu FR

doi

10.1167/iovs.11-8184

subject

Has Abstract

pub_date

2012-09-21 00:00:00

pages

6416-26

issue

10

eissn

0146-0404

issn

1552-5783

pii

iovs.11-8184

journal_volume

53

pub_type

杂志文章
  • Isolation and characterization of mesenchymal progenitor cells from human orbital adipose tissue.

    abstract:PURPOSE:Adipose-derived stem cells (ASCs) have gained importance due to their myriad potential clinical applications. We hypothesize that progenitor cells also exist besides those conventionally isolated from the stromal vascular fraction (SVF). METHOD:Central and medial orbital adipose tissues obtained from patients ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-14441

    authors: Chen SY,Mahabole M,Horesh E,Wester S,Goldberg JL,Tseng SC

    更新日期:2014-07-03 00:00:00

  • Gamma-interferon effects on human fibroblasts from Tenon's capsule.

    abstract::Gamma-interferon (G-IFN) exhibits antiproliferative effects, inhibits collagen synthesis by fibroblasts, and may help to regulate the turnover of fibrous tissue. Fibroblasts from Tenon's capsule are cellular components that contribute to unsuccessful glaucoma filtration surgery. Therefore, the authors studied the effe...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Latina MA,Belmonte SJ,Park C,Crean E

    更新日期:1991-09-01 00:00:00

  • Photoreceptor protection by cardiotrophin-1 in transgenic rats with the rhodopsin mutation s334ter.

    abstract:PURPOSE:This work examines the effects of cardiotrophin (CT)-1 on photoreceptor survival in transgenic rats that carry the rhodopsin mutation S334ter. METHODS:Recombinant CT-1 was injected intravitreally into eyes of heterozygous animals. Photoreceptor survival was analyzed by histology. Phosphorylation of signal tran...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.02-1130

    authors: Song Y,Zhao L,Tao W,Laties AM,Luo Z,Wen R

    更新日期:2003-09-01 00:00:00

  • Diclofenac protects cultured human corneal epithelial cells against hyperosmolarity and ameliorates corneal surface damage in a rat model of dry eye.

    abstract:PURPOSE:Dry eye syndrome (DES) is characterized by an increase in tear osmolarity and induction of the expression and nuclear localization of an osmoprotective transcription factor (nuclear factor of activated T-cells 5 [NFAT5]) that plays an important role in providing protection against hyperosmotic tears. In this st...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-13850

    authors: Sawazaki R,Ishihara T,Usui S,Hayashi E,Tahara K,Hoshino T,Higuchi A,Nakamura S,Tsubota K,Mizushima T

    更新日期:2014-04-21 00:00:00

  • Genotype-phenotype correlation of mouse pde6b mutations.

    abstract:PURPOSE:To identify the underlying molecular defects causing retinal degeneration in seven N-ethyl-N-nitrosourea (ENU) induced mutant alleles of the Pde6b gene and to analyze the timescale of retinal degeneration in these new models of retinitis pigmentosa. METHODS:Conformation sensitive capillary electrophoresis and ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.05-0254

    authors: Hart AW,McKie L,Morgan JE,Gautier P,West K,Jackson IJ,Cross SH

    更新日期:2005-09-01 00:00:00

  • Effects of Diabetes on Mitochondrial Morphology and Its Implications in Diabetic Retinopathy.

    abstract:Purpose:To determine whether high glucose (HG) or diabetes alters mitochondrial morphology and promotes mitochondrial fragmentation in retinal vascular cells and thereby triggers apoptosis associated with diabetic retinopathy. Methods:To assess whether diabetes promotes mitochondrial fragmentation and thereby triggers...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.61.10.10

    authors: Kim D,Roy S

    更新日期:2020-08-03 00:00:00

  • Autosomal recessive retinitis pigmentosa is associated with mutations in RP1 in three consanguineous Pakistani families.

    abstract:PURPOSE:To localize and identify the gene and mutations causing autosomal recessive retinitis pigmentosa in three consanguineous Pakistani families. METHODS:Blood samples were collected and DNA was extracted. A genome-wide scan was performed by using 382 polymorphic microsatellite markers on genomic DNA from affected ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.04-1280

    authors: Riazuddin SA,Zulfiqar F,Zhang Q,Sergeev YV,Qazi ZA,Husnain T,Caruso R,Riazuddin S,Sieving PA,Hejtmancik JF

    更新日期:2005-07-01 00:00:00

  • Effect of intravitreal injection of high-dose bevacizumab in monkey eyes.

    abstract:PURPOSE:To evaluate the ocular complications of intravitreal injection of high dose bevacizumab in monkey eyes. METHODS:Four healthy monkeys (Macaca fuscata) received an intravitreal injection of either 6.25 or 12.5 mg bevacizumab in the right eye; the left control eye received an intravitreal injection of the same vo...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.07-1542

    authors: Sakurai K,Akiyama H,Shimoda Y,Yoshida I,Kurabayashi M,Kishi S

    更新日期:2009-10-01 00:00:00

  • Dynamic patterns of histone lysine methylation in the developing retina.

    abstract:PURPOSE:Histone lysine methylation (HKM) is an important epigenetic mechanism that establishes cell-specific gene expression and functions in development. However, epigenetic control of retinal development is poorly understood. To study the roles of HKM in retinogenesis, the authors examined the dynamic changes of thre...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.09-4730

    authors: Rao RC,Tchedre KT,Malik MT,Coleman N,Fang Y,Marquez VE,Chen DF

    更新日期:2010-12-01 00:00:00

  • Dye coupling of rabbit ciliary epithelial cells in vitro.

    abstract:PURPOSE:To examine the in vitro properties of gap junctions on the isolated rabbit ciliary epithelium. METHODS:Intracellular potential was measured and lucifer yellow (5% in 1 M LiCl) was iontophoretically injected into a ciliary epithelial cell. Fixated tissue was examined with a laser confocal microscope. RESULTS:D...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Oh J,Krupin T,Tang LQ,Sveen J,Lahlum RA

    更新日期:1994-04-01 00:00:00

  • Sialoglycoproteins of the frog rod outer segment plasma membrane.

    abstract::Photoreceptors can segregate inner segment proteins from outer segment proteins. It is uncertain whether such a sorting process occurs during disc morphogenesis in rods, thereby resulting in an outer segment plasma membrane which differs compositionally from the discs. In this study, the plasma membranes of intact, pu...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Polans AS,Burton MD

    更新日期:1988-10-01 00:00:00

  • Activation of multiple pathways during photoreceptor apoptosis in the rd mouse.

    abstract:PURPOSE:The primary purpose of this study was to characterize photoreceptor apoptosis in the rd mouse. Given that apoptosis is the final common pathway in many cases of retinal degeneration, the ability to retard or even arrest this process may ameliorate retinal disorders such as retinitis pigmentosa (RP). The absence...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.05-0248

    authors: Doonan F,Donovan M,Cotter TG

    更新日期:2005-10-01 00:00:00

  • In situ localization of cytoskeletal elements in the human trabecular meshwork and cornea.

    abstract::The authors compared cytoskeletal elements of the in situ human trabecular-meshwork cell with in situ human corneal cells using indirect immunofluorescence staining for tubulin and intermediate filaments (vimentin, cytokeratin, and desmin) and NBD-phallacidin staining for f-actin using both fixed frozen and unfixed fr...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Weinreb RN,Ryder MI

    更新日期:1990-09-01 00:00:00

  • The ocular hypotensive effects of demeclocycline, tetracycline and other tetracycline derivatives.

    abstract::Demeclocycline, tetracycline and other tetracycline derivatives lowered intraocular pressure (IOP) in rabbits following intravitreal injection, but the onset of this effect was not evident until 1 or more days after drug administration. Of the drugs tested, demeclocycline was the most active ocular hypotensive agent. ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Wallace I,Krupin T,Stone RA,Moolchandani J

    更新日期:1989-07-01 00:00:00

  • The Effect of Age on the Temporal Summation of Achromatic Perimetric Stimuli.

    abstract:PURPOSE:To examine the temporal summation of a Goldmann III-sized stimulus under the conditions of standard automated perimetry in healthy participants of varying age. METHODS:Twenty-seven healthy individuals of varying age (24-80 years) were tested. Achromatic contrast thresholds were measured for seven 0.48° diamete...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.15-16920

    authors: Mulholland PJ,Redmond T,Garway-Heath DF,Zlatkova MB,Anderson RS

    更新日期:2015-10-01 00:00:00

  • Involvement of the Erk-MAP kinase pathway in TNFalpha regulation of trabecular matrix metalloproteinases and TIMPs.

    abstract:PURPOSE:TNFalpha is a strong modulator expression of trabecular meshwork (TM) matrix metalloproteinase (MMP) and tissue inhibitor (TIMP). Laser trabeculoplasty appears to rely on this process to restore normal aqueous humor outflow facility. Thus, studies were conducted to determine whether the extracellular signal-reg...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.01-1201

    authors: Alexander JP,Acott TS

    更新日期:2003-01-01 00:00:00

  • Prior binocular dissociation reduces monocular form deprivation amblyopia in monkeys.

    abstract::The profound visual deficits associated with early monocular form deprivation (MD) are caused largely by competitive binocular interactions in the visual cortex. We tested the hypothesis that disrupting normal cortical binocularity prior to the onset of MD would reduce the degree of form deprivation amblyopia compared...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Smith EL 3rd,Harwerth RS,Siderov J,Wingard M,Crawford ML,von Noorden GK

    更新日期:1992-04-01 00:00:00

  • Origin and biosynthesis of human tear fluid proteins.

    abstract::In tear fluid, a large number of proteins can be detected with electrophoretic or immunologic techniques. The composition of serum proteins in tears resembles that of whole serum. By comparison of the protein patterns resulting from different sampling methods, it was shown that serum albumin is not present in the secr...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Janssen PT,van Bijsterveld OP

    更新日期:1983-05-01 00:00:00

  • Endothelial nitric oxide synthase-related mechanotransduction changes in aged porcine angular aqueous plexus cells.

    abstract:PURPOSE:To investigate effects of aging on endothelial nitric oxide synthase (eNOS) expression and signaling in angular aqueous plexus (AAP) (functional equivalent to human Schlemm's canal) cells subjected to shear stress. METHODS:The AAP cells were isolated differentially from porcine outflow tissues using puromycin ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-14992

    authors: Lei Y,Stamer WD,Wu J,Sun X

    更新日期:2014-11-06 00:00:00

  • Quantitative Fundus Autofluorescence in Rhesus Macaques in Aging and Age-Related Drusen.

    abstract:Purpose:To employ quantitative fundus autofluorescence (qAF) imaging in rhesus macaques to noninvasively assess retinal pigment epithelial (RPE) lipofuscin in nonhuman primates (NHPs) as a model of aging and age-related macular degeneration (AMD). Methods:The qAF imaging was performed on eyes of 26 rhesus macaques (me...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.61.8.16

    authors: Tran TM,Kim S,Lin KH,Chung SH,Park S,Sazhnyev Y,Wang Y,Cunefare D,Farsiu S,Thomasy SM,Moshiri A,Yiu G

    更新日期:2020-07-01 00:00:00

  • Retinal nerve fiber layer normative classification by optical coherence tomography for prediction of future visual field loss.

    abstract:PURPOSE:To evaluate the utility of baseline Stratus optical coherence tomography (OCT; Carl Zeiss Meditec, Dublin, CA) retinal nerve fiber layer (RNFL) normative classification in the prediction of future visual field (VF) loss. METHODS:Eighty-eight eyes with suspected glaucoma with abnormal RNFL classification by Str...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-6246

    authors: Sung KR,Kim S,Lee Y,Yun SC,Na JH

    更新日期:2011-04-22 00:00:00

  • Collagen gel contraction by cells associated with proliferative vitreoretinopathy.

    abstract::The capacities of porcine choroidal fibroblasts, retinal glial cells, and retinal pigment epithelial cells to contract collagen gels in vitro were compared. Experiments with varied cell numbers indicated that glial cells are the most effective, followed by choroidal fibroblasts and retinal pigment epithelial cells. An...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Guidry C,McFarland RJ,Morris R,Witherspoon CD,Hook M

    更新日期:1992-07-01 00:00:00

  • Histopathology of delayed hypersensitivity reacitons in the guinea pig uveal tract.

    abstract::Two distinct patterns of delayed-onset, cell-mediated hypersensitivity have been induced in the guinea pig eye with the same soluble protein antigen and two different methods of immunization. The predominant histologic feature of these reactions was the large number of eosinophils which infiltrated the uveal tract and...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Friedlaender MH,Howes EL Jr,Hall JM,Krasnobrod H,Wormstead MA

    更新日期:1978-04-01 00:00:00

  • Repetitive nonlethal oxidant injury to retinal pigment epithelium decreased extracellular matrix turnover in vitro and induced sub-RPE deposits in vivo.

    abstract:PURPOSE:To determine the impact of repetitive nonlethal oxidant injury with hydroquinone (HQ) on regulation of cell membrane blebbing and molecules, which are essential in extracellular matrix turnover (ECM) maintenance, especially matrix metalloproteinase (MMP)-2, tissue inhibitor of MMP (TIMP)-2, and type IV collagen...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.05-1230

    authors: Marin-Castaño ME,Striker GE,Alcazar O,Catanuto P,Espinosa-Heidmann DG,Cousins SW

    更新日期:2006-09-01 00:00:00

  • An assessment of polymorphonuclear leukocyte rigidity in HIV-infected individuals after immune recovery.

    abstract:PURPOSE:To determine whether polymorphonuclear leukocytes (PMNs) remain rigid after immune reconstitution in human immunodeficiency virus (HIV)-infected individuals with a history of severe immunosuppression. METHODS:PMN rigidity was measured in vitro in three groups: (1) HIV-infected individuals with a history of CD4...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Goldenberg DT,Holland GN,Cumberland WG,Fisher TC,Folz IC,Wang RC,Terry BG,Moe AA,Kramer F,Lim JI,Rao NA,Meiselman HJ

    更新日期:2002-06-01 00:00:00

  • Optic disc progression in glaucoma: comparison of confocal scanning laser tomography to optic disc photographs in a prospective study.

    abstract:PURPOSE:To compare confocal scanning laser tomography (CSLT) to optic disc photography for detecting progressive glaucomatous disc changes. METHODS:Four expert observers first examined a training set of 40 pairs of monoscopic disc photographs to help set a criterion for change. They then classified 44 pairs of monosco...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.08-2457

    authors: Chauhan BC,Hutchison DM,Artes PH,Caprioli J,Jonas JB,LeBlanc RP,Nicolela MT

    更新日期:2009-04-01 00:00:00

  • Transgenic mice expressing mutated Tyr437His human myocilin develop progressive loss of retinal ganglion cell electrical responsiveness and axonopathy with normal iop.

    abstract:PURPOSE:To characterize age-related changes of retinal ganglion cell (RGC) function, IOP, and anatomical markers of axon/glia integrity in a transgenic mouse expressing Tyr437His mutant of human myocilin protein. METHODS:Retinal ganglion cell electrical responsiveness was tested with pattern electroretinogram (PERG) i...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-14793

    authors: Chou TH,Tomarev S,Porciatti V

    更新日期:2014-08-14 00:00:00

  • The roles of vitreal macrophages and circulating leukocytes in retinal neovascularization.

    abstract:PURPOSE:To analyze the roles of vitreal macrophages and circulating leukocytes in retinal vascular growth. METHODS:Bone marrow (BM) cells from green fluorescent protein (GFP) transgenic mice were transplanted into postnatal day (P)1 mice after irradiation. The mice were exposed to 76% to 78% oxygen (P7-P12), to initia...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-5798

    authors: Kataoka K,Nishiguchi KM,Kaneko H,van Rooijen N,Kachi S,Terasaki H

    更新日期:2011-03-14 00:00:00

  • Heparanase and vascular endothelial growth factor expression is increased in hypoxia-induced retinal neovascularization.

    abstract:PURPOSE:Heparanase and VEGF are related closely to angiogenesis in cancer. The purpose of our study was to evaluate the expression and correlation of heparanase and VEGF in hypoxia-induced retinal neovascularization. METHODS:C57BL/6 oxygen-induced retinopathy (OIR) mice and human retinal microvascular endothelial cell...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-9144

    authors: Hu J,Song X,He YQ,Freeman C,Parish CR,Yuan L,Yu H,Tang S

    更新日期:2012-10-03 00:00:00

  • Neuronal integration in an abutting-retinas culture system.

    abstract:PURPOSE:Limited integration is consistently observed between subretinal transplants and host retinas. In the current study, an in vitro model system for studying connections forming between two abutting retinas was developed. METHODS:Neuroretinas were dissected from normal wild-type (WT) mice and green fluorescent pro...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.02-0640

    authors: Zhang Y,Caffé AR,Azadi S,van Veen T,Ehinger B,Perez MT

    更新日期:2003-11-01 00:00:00