Abstract:
:Pupylation is a posttranslational protein modification occurring in mycobacteria and other actinobacteria that is functionally analogous to ubiquitination. Here we report the crystal structures of the modification enzymes involved in this pathway, the prokaryotic ubiquitin-like protein (Pup) ligase PafA and the depupylase/deamidase Dop. Both feature a larger amino-terminal domain consisting of a central β-sheet packed against a cluster of helices, a fold characteristic for carboxylate-amine ligases, and a smaller C-terminal domain unique to PafA/Dop members. The active site is located on the concave surface of the β-sheet with the nucleotide bound in a deep pocket. A conserved groove leading into the active site could have a role in Pup-binding. Nuclear magnetic resonance and biochemical experiments determine the region of Pup that interacts with PafA and Dop. Structural data and mutational studies identify crucial residues for the catalysis of both enzymes.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Özcelik D,Barandun J,Schmitz N,Sutter M,Guth E,Damberger FF,Allain FH,Ban N,Weber-Ban Edoi
10.1038/ncomms2009subject
Has Abstractpub_date
2012-01-01 00:00:00pages
1014issn
2041-1723pii
ncomms2009journal_volume
3pub_type
杂志文章abstract::Transcriptional regulation by chromatin is a highly dynamic process directed through the recruitment and coordinated action of epigenetic modifiers and readers of these modifications. Using an unbiased proteomic approach to find interactors of H3K36me3, a modification enriched on active chromatin, here we identify PWW...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06235-9
更新日期:2018-09-18 00:00:00
abstract::Large single-crystal graphene is highly desired and important for the applications of graphene in electronics, as grain boundaries between graphene grains markedly degrade its quality and properties. Here we report the growth of millimetre-sized hexagonal single-crystal graphene and graphene films joined from such gra...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1702
更新日期:2012-02-28 00:00:00
abstract::Microbial fermentation of lignocellulosic biomass to produce industrial chemicals is exacerbated by the recalcitrant network of lignin, cellulose and hemicelluloses comprising the plant secondary cell wall. In this study, we show that transgenic poplar (Populus trichocarpa) lines can be solubilized without any pretrea...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11376-6
更新日期:2019-08-07 00:00:00
abstract::Novel atherosclerosis models are needed to guide clinical therapy. Here, we report an in vitro model of early atherosclerosis by fabricating and perfusing multi-layer arteriole-scale human tissue-engineered blood vessels (TEBVs) by plastic compression. TEBVs maintain mechanical strength, vasoactivity, and nitric oxide...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-19197-8
更新日期:2020-10-27 00:00:00
abstract::During apoptosis, Bak and Bax are activated by BH3-only proteins binding to the α2-α5 hydrophobic groove; Bax is also activated via a rear pocket. Here we report that antibodies can directly activate Bak and mitochondrial Bax by binding to the α1-α2 loop. A monoclonal antibody (clone 7D10) binds close to α1 in non-act...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11734
更新日期:2016-05-24 00:00:00
abstract::Decision-making is often accompanied by a degree of confidence on whether a choice is correct. Decision uncertainty, or lack in confidence, may lead to change-of-mind. Studies have identified the behavioural characteristics associated with decision confidence or change-of-mind, and their neural correlates. Although se...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10316-8
更新日期:2019-05-23 00:00:00
abstract::In the development of addiction, drug seeking becomes habitual and controlled by drug-associated cues, and the neural locus of control over behaviour shifts from the ventral to the dorsolateral striatum. The neural mechanisms underlying this functional transition from recreational drug use to drug-seeking habits are u...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10088
更新日期:2015-12-14 00:00:00
abstract::In genome editing with CRISPR-Cas9, transgene integration often remains challenging. Here, we present an approach for increasing the efficiency of transgene integration by homology-dependent repair (HDR). CtIP, a key protein in early steps of homologous recombination, is fused to Cas9 and stimulates transgene integrat...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03475-7
更新日期:2018-03-19 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature communications
pub_type: 已发布勘误
doi:10.1038/s41467-019-11801-w
更新日期:2019-08-19 00:00:00
abstract::Antibiotic biosynthetic gene clusters (BGCs) produce bioactive metabolites that impart a fitness advantage to their producer, providing a mechanism for natural selection. This selection drives antibiotic evolution and adapts BGCs for expression in different organisms, potentially providing clues to improve heterologou...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18018-2
更新日期:2020-08-21 00:00:00
abstract::Antibodies against programmed cell death-1 (PD-1) have considerably changed the treatment for melanoma. However, many patients do not display therapeutic response or eventually relapse. Moreover, patients treated with anti-PD-1 develop immune-related adverse events that can be cured with anti-tumor necrosis factor α (...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02358-7
更新日期:2017-12-22 00:00:00
abstract::The interband cascade laser differs from any other class of semiconductor laser, conventional or cascaded, in that most of the carriers producing population inversion are generated internally, at semimetallic interfaces within each stage of the active region. Here we present simulations demonstrating that all previous...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1595
更新日期:2011-12-13 00:00:00
abstract::The mammary gland is a highly vascularized tissue capable of expansion and regression during development and disease. To enable mechanistic insight into the coordinated morphogenic crosstalk between the epithelium and vasculature, we introduce a 3D microfluidic platform that juxtaposes a human mammary duct in proximit...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17102-x
更新日期:2020-07-06 00:00:00
abstract::The social intelligence hypothesis (SIH) posits that within-group interactions drive cognitive evolution, but it has received equivocal support. We argue the SIH overlooks a major component of social life: interactions with conspecific outsiders. Competition for vital resources means conspecific outsiders present myri...
journal_title:Nature communications
pub_type: 杂志文章,评审
doi:10.1038/s41467-020-18780-3
更新日期:2020-10-06 00:00:00
abstract::Droplet interactions with compliant materials are familiar, but surprisingly complex processes of importance to the manufacturing, chemical, and garment industries. Despite progress-previous research indicates that mesoscopic substrate deformations can enhance droplet drying or slow down spreading dynamics-our underst...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12093-w
更新日期:2019-10-21 00:00:00
abstract::Efficient charge separation is of crucial importance for the improvement of photocatalytic activity for solar hydrogen evolution. Here we report efficient photo-generated charge separation by twin-induced one-dimensional homojunctions with type-II staggered band alignment, using a ternary chalcogenate, i.e. Cd0.5Zn0.5...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3278
更新日期:2013-01-01 00:00:00
abstract::The fields of soft polymers and macromolecular sciences have enjoyed a unique combination of metals and organic frameworks in the name of metallopolymers or organometallic polymers. When metallopolymers carry charged groups, they form a class of metal-containing polyelectrolytes or metallo-polyelectrolytes. This revie...
journal_title:Nature communications
pub_type: 杂志文章,评审
doi:10.1038/s41467-018-06475-9
更新日期:2018-10-18 00:00:00
abstract::Long nanopore reads are advantageous in de novo genome assembly. However, nanopore reads usually have broad error distribution and high-error-rate subsequences. Existing error correction tools cannot correct nanopore reads efficiently and effectively. Most methods trim high-error-rate subsequences during error correct...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20236-7
更新日期:2021-01-04 00:00:00
abstract::The original version of this Article contained errors in Fig. 3. In panel a, bars from a chart depicting the percentage of antibody-positive individuals in non-infectious and infectious groups were inadvertently included in place of bars depicting the percentage of infectious individuals, as described in the Article a...
journal_title:Nature communications
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41467-018-03769-w
更新日期:2018-04-11 00:00:00
abstract::Highly sensitive and specific non-invasive molecular imaging methods are particularly desirable for the early detection of cancers. Here we report a near-infrared optical imaging probe highly specific to the hypoxic tumour microenvironment to detect tumour and cancer cells with the sensitivity to a few thousands cance...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6834
更新日期:2015-01-05 00:00:00
abstract::Grains and glasses, widely different materials, arrest their motions upon decreasing temperature and external load, respectively, in common ways, leading to a universal jamming phase diagram conjecture. However, unified theories are lacking, mainly because of the disparate nature of the particle interactions. Here we ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2177
更新日期:2012-01-01 00:00:00
abstract::Early detection and accurate monitoring of chronic kidney disease (CKD) could improve care and retard progression to end-stage renal disease. Here, using untargeted metabolomics in 2155 participants including patients with stage 1-5 CKD and healthy controls, we identify five metabolites, including 5-methoxytryptophan ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09329-0
更新日期:2019-04-01 00:00:00
abstract::Ciliary membrane composition is controlled by transition zone (TZ) proteins such as RPGRIP1, RPGRIPL and NPHP4, which are vital for balanced coordination of diverse signalling systems like the Sonic hedgehog (Shh) pathway. Activation of this pathway involves Shh-induced ciliary accumulation of Smoothened (SMO), which ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14177
更新日期:2017-01-30 00:00:00
abstract::Nanostructured metasurfaces offer unique capabilities for subwavelength control of optical waves. Based on this potential, a large number of metasurfaces have been proposed recently as alternatives to standard optical elements. In most cases, however, these elements suffer from large chromatic aberrations, thus limiti...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14992
更新日期:2017-04-05 00:00:00
abstract::The growth of atomically dispersed metal catalysts (ADMCs) remains a great challenge owing to the thermodynamically driven atom aggregation. Here we report a surface-limited electrodeposition technique that uses site-specific substrates for the rapid and room-temperature synthesis of ADMCs. We obtained ADMCs by the un...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18430-8
更新日期:2020-09-11 00:00:00
abstract::Although networks are extensively used to visualize information flow in biological, social and technological systems, translating topology into dynamic flow continues to challenge us, as similar networks exhibit fundamentally different flow patterns, driven by different interaction mechanisms. To uncover a network's a...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01916-3
更新日期:2017-12-19 00:00:00
abstract::Activation of innate immunity by membrane-localized receptors is conserved across eukaryotes. Plant genomes contain hundreds of such receptor-like genes and those encoding proteins with an extracellular leucine-rich repeat (LRR) domain represent the largest family. Here, we develop a high-throughput approach to study ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03010-8
更新日期:2018-02-09 00:00:00
abstract::Chromothripsis is a recently identified mutational phenomenon, by which a presumably single catastrophic event generates extensive genomic rearrangements of one or a few chromosome(s). Considered as an early event in tumour development, this form of genome instability plays a prominent role in tumour onset. Chromothri...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16134-7
更新日期:2020-05-08 00:00:00
abstract::Genomic alterations occurring during melanoma progression and the resulting genomic heterogeneity between metastatic deposits remain incompletely understood. Analyzing 86 metastatic melanoma deposits from 53 patients with whole-exome sequencing (WES), we show a low branch to trunk mutation ratio and little intermetast...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05063-1
更新日期:2018-07-10 00:00:00
abstract::Th9 cells protect hosts against helminthic infection but also mediate allergic disease. Here we show that nitric oxide (NO) promotes Th9 cell polarization of murine and human CD4(+) T cells. NO de-represses the tumour suppressor gene p53 via nitrosylation of Mdm2. NO also increases p53-mediated IL-2 production, STAT5 ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5575
更新日期:2014-08-07 00:00:00