Involvement of ROS in the inhibitory effect of thermotherapy combined with chemotherapy on A549 human lung adenocarcinoma cell growth through the Akt pathway.

Abstract:

:Mechanisms of synergistic effect of thermotherapy and chemotherapy on human lung adenocarcinoma cell growth are unclear. The purpose of this study was to investigate these effects and explore the function of ROS, Akt and caspase-3 in relation to these. A549 cells were subjected to different thermochemotherapy treatments: 43˚C heat+50 µg/l paclitaxel (thermochemotherapy group), 43˚C heat+50 µg/l paclitaxel+1 µmol/l wortmannin (wortmannin group), 43˚C heat+50 µg/l paclitaxel+30 µmol/l NAC (NAC group) and 50 µg/l paclitaxel (chemotherapy group). The cells without any treatment were regarded as controls. Cell proliferation rates were measured with MTT assay and intracellular ROS levels were detected with fluorescence labeling technologies. Phosphorylation of Akt and caspase-3 expression were determined by western blotting and the cell apoptosis rates were examined by flow cytometry. Tests in vivo were carried out at the same time. It was found that the cell proliferation rates of the thermochemotherapy group were significantly lower compared to those of the controls or the chemotherapy group (P<0.05). The intracellular ROS levels of the thermochemotherapy group were elevated significantly compared with those of other groups, and these changes could be reversed using the ROS inhibitor NAC but not a PI3K inhibitor (wortmannin). Phosphorylation of Akt was significantly decreased in the thermochemotherapy group (P<0.05), which could be blocked by wortmannin, but increased by NAC (P<0.05). The caspase-3 expression levels and cell apoptosis rates of the thermochemotherapy group were higher compared to those of the other groups (P<0.05). All results have been confirmed by in vivo tests. Thus, the combination of thermotherapy with chemotherapy showed a stronger inhibitory effect on A549 cell growth compared to chemotherapy alone, which may be able to cause additional cell apoptosis through inhibition of Akt phosphorylation and activation of caspases by increased intracellular ROS production.

journal_name

Oncol Rep

journal_title

Oncology reports

authors

Wang L,Liu X,Wu Y,Wu W,Wu Y

doi

10.3892/or.2012.1954

subject

Has Abstract

pub_date

2012-10-01 00:00:00

pages

1369-75

issue

4

eissn

1021-335X

issn

1791-2431

journal_volume

28

pub_type

杂志文章
  • Epigenetic changes of tumor suppressor genes, P15, P16, VHL and P53 in oral cancer.

    abstract::We performed methylation specific PCR to explore the mechanism of inactivation of tumor suppressor genes P15, P16, P53 and VHL in 48 oral SCC. The frequencies of aberrant methylation on the promoter of the P15, the P16, the P53 and the VHL genes were 0.27 (13/48), 0.42 (20/48), 0.04 (2/48) and none, respectively. Alto...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Yeh KT,Chang JG,Lin TH,Wang YF,Tien N,Chang JY,Chen JC,Shih MC

    更新日期:2003-05-01 00:00:00

  • Involvement of TMEM22 overexpression in the growth of renal cell carcinoma cells.

    abstract::In order to clarify the molecular mechanism involved in renal carcinogenesis, and to identify molecular targets for development of novel treatments of renal cell carcinoma (RCC), we previously analyzed genome-wide gene expression profiles of clear-cell types of RCC by cDNA microarray. Among the transcativated genes, w...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Dobashi S,Katagiri T,Hirota E,Ashida S,Daigo Y,Shuin T,Fujioka T,Miki T,Nakamura Y

    更新日期:2009-02-01 00:00:00

  • Characterization of the prostaglandin biosynthetic pathway in non-small cell lung cancer: a comparison with small cell lung cancer and correlation with angiogenesis, angiogenic factors and metastases.

    abstract::To investigate the components of the prostaglandin biosynthetic pathway, the characteristics of cyclooxygenase (COX)-1, COX-2, hematopoietic prostaglandin D synthase (hPGDS), microsomal prostaglandin E synthase (mPGES), and thromboxane synthase (TXS) were examined for autopsied cases with small cell lung cancer (SCLC)...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Yoshimoto A,Kasahara K,Kawashima A,Fujimura M,Nakao S

    更新日期:2005-06-01 00:00:00

  • Tanshinone IIA can inhibit MiaPaCa‑2 human pancreatic cancer cells by dual blockade of the Ras/Raf/MEK/ERK and PI3K/AKT/mTOR pathways.

    abstract::Tanshinone IIA (Tan‑IIA; C19H18O3) is derived from Danshen (the roots of Salvia miltiorrhiza), and has been reported to possess anti‑inflammatory and antioxidant activities. Tan‑IIA can inhibit BxPC‑3 human pancreatic cancer cells in vitro through inducing endoplasmic reticulum stress and apoptosis via mitochondrial p...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6670

    authors: Su CC

    更新日期:2018-11-01 00:00:00

  • Survivin downregulation by siRNA sensitizes human hepatoma cells to TRAIL-induced apoptosis.

    abstract::Survivin, an anti-apoptotic protein, is abundantly expressed in a variety of cancer cells, including hepatoma cells, resulting in the resistance of these cells to various apoptotic stimuli. Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is known to induce cancer cell-specific apoptosis, but hepa...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Nakao K,Hamasaki K,Ichikawa T,Arima K,Eguchi K,Ishii N

    更新日期:2006-08-01 00:00:00

  • Resveratrol enhances the radiosensitivity of nasopharyngeal carcinoma cells by downregulating E2F1.

    abstract::Identification of safe, effective radiosensitizing agents is urgently needed to improve the outcome of radiotherapy in nasopharyngeal cancer (NPC). In this study, we assessed the ability of the polyphenol resveratrol to act as a radiosensitizer in vitro and in vivo. CNE-1 cells were treated with 50 µM resveratrol for ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5413

    authors: Tan Y,Wei X,Zhang W,Wang X,Wang K,Du B,Xiao J

    更新日期:2017-03-01 00:00:00

  • Expression analysis of pancreatic cancer cell lines reveals association of enhanced gene transcription and genomic amplifications at the 8q22.1 and 8q24.22 loci.

    abstract::Despite tremendous effort and progress in the diagnostics of pancreatic cancer with respect to imaging techniques and molecular genetics, only very few patients can be cured by surgery leading to a 5-year survival rate of only 3%. Especially the lack of chemotherapeutical options in this entity requires a better under...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Domagk D,Schaefer KL,Eisenacher M,Braun Y,Wai DH,Schleicher C,Diallo-Danebrock R,Bojar H,Roeder G,Gabbert HE,Domschke W,Poremba C

    更新日期:2007-02-01 00:00:00

  • NEK2 promotes hepatocellular carcinoma migration and invasion through modulation of the epithelial-mesenchymal transition.

    abstract::Never in mitosis gene-A (NIMA)-related expressed kinase 2 (NEK2) has been recently reported to play a role in tumor progression, drug resistance and tumorigenesis. However, little is known about the effects of NEK2 in hepatocellular carcinoma (HCC) metastasis and the underlying mechanism. NEK2 expression levels were e...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6224

    authors: Zhang Y,Wang W,Wang Y,Huang X,Zhang Z,Chen B,Xie W,Li S,Shen S,Peng B

    更新日期:2018-03-01 00:00:00

  • Rejection of adenovirus infection is independent of coxsackie and adenovirus receptor expression in cisplatin-resistant human lung cancer cells.

    abstract::The adenovirus vector-based cancer gene therapy is controversial. Low transduction efficacy is believed to be one of the main barriers for the decreased expression of coxsackie and adenovirus receptor (CAR) on tumor cells. However, the expression of CAR on primary tumor tissue and tumor tissue survived from treatment ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4870

    authors: Zhang NH,Peng RQ,Ding Y,Zhang XS

    更新日期:2016-08-01 00:00:00

  • Hepatitis B virus X protein mutant upregulates CENP-A expression in hepatoma cells.

    abstract::The carcinogenic role of hepatitis B virus x protein (HBx) in hepatocellular carcinoma (HCC) remains largely unknown. Centromere protein A (CENP-A) has been found to be frequently overexpressed in HCC. In the present study, we aimed to investigate the role of HBx in regulating CENP-...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1478

    authors: Liu L,Li Y,Zhang S,Yu D,Zhu M

    更新日期:2012-01-01 00:00:00

  • Phase I study of S-1 and biweekly docetaxel combination chemotherapy for advanced and recurrent gastric cancer.

    abstract::A phase I study of S-1 and biweekly docetaxel (DOC) combination therapy was conducted to determine the maximum tolerated dose (MTD) and pharmacokinetic parameters. Fourteen patients with advanced or recurrent gastric cancer were analyzed. The treatment consisted of S-1 [body surface area (BSA) <1.25 m2:80 mg/day, 1.25...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Takahashi I,Emi Y,Kakeji Y,Tokunaga E,Ushiro S,Oki E,Watanabe M,Baba H,Maehara Y

    更新日期:2006-04-01 00:00:00

  • A somatic TSHR mutation in a patient with lung adenocarcinoma with bronchioloalveolar carcinoma, coronary artery disease and severe chronic obstructive pulmonary disease.

    abstract::In a screen for thoracic malignancy-associated markers, thyroid stimulating hormone receptor (TSHR) was identified as a candidate as it binds to the previously-characterized lung cancer marker NKX2-1. We screened for mutations in all coding regions of the TSHR gene in 96 lung adenoc...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.1938

    authors: Kim JW,Lee S,Lui N,Choi H,Mulvihill M,Fang LT,Kang HC,Kwon YW,Jablons D,Kim IJ

    更新日期:2012-10-01 00:00:00

  • Honeybee venom possesses anticancer and antiviral effects by differential inhibition of HPV E6 and E7 expression on cervical cancer cell line.

    abstract::Bee venom (BV) therapy is a type of alternative medical treatment used to treat various diseases in oriental medicine. The mechanisms underlying the effects of BV remain poorly understood. In the present study, we evaluated the antiviral effect of BV on cervical carcinoma cell lines (CaSki, HeLa, C33A and TC-1). BV tr...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.3760

    authors: Kim YW,Chaturvedi PK,Chun SN,Lee YG,Ahn WS

    更新日期:2015-04-01 00:00:00

  • Evaluation of the STAT3 inhibitor GLG‑302 for the prevention of estrogen receptor‑positive and ‑negative mammary cancers.

    abstract::Signal transducer and activator of transcription 3 (STAT3) plays a key role in the transformation of normal cells to cancerous cells. Although inhibitors of STAT3 have been shown to suppress the growth of multiple cancer types in vitro and in vivo, such agents are of particular interest for the prevention of breast ca...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7219

    authors: Shoemaker RH,Fox JT,Juliana MM,Moeinpour FL,Grubbs CJ

    更新日期:2019-09-01 00:00:00

  • Genetically modified tumour vaccines producing IL-12 augment chemotherapy of HPV16-associated tumours with gemcitabine.

    abstract::Genetically modified tumour cells producing cytokines such as interleukin 12 (IL-12) are potent activators of the antitumour immune responses and represent a promising therapeutic modality when combined with chemotherapy. The objective of this study was to examine whether IL-12-producing cellular vaccines can augment ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1221

    authors: Mikyšková R,Indrová M,Símová J,Bieblová J,Bubeník J,Reiniš M

    更新日期:2011-06-01 00:00:00

  • Paclitaxel enhances tumoricidal potential of TRAIL via inhibition of MAPK in resistant gastric cancer cells.

    abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) holds promise for cancer therapy due to its unique capacity to selectively trigger apoptosis in cancer cells. However, TRAIL therapy is greatly hampered by its resistance. A preclinical successful strategy is to identify combination treatments that sensit...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4666

    authors: Li L,Wen XZ,Bu ZD,Cheng XJ,Xing XF,Wang XH,Zhang LH,Guo T,Du H,Hu Y,Fan B,Ji JF

    更新日期:2016-05-01 00:00:00

  • Bmi-1 induces radioresistance in MCF-7 mammary carcinoma cells.

    abstract::Bmi-1, a member of the polycomb family, it is involved in self renewal of stem cells and functions as an oncogene in many malignant human cancer types. Recent studies have demonstrated that Bmi-1 is a predictive factor for poor patient prognosis. However, the underlying mechanisms o...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1615

    authors: Liu ZG,Liu L,Xu LH,Yi W,Tao YL,Tu ZW,Li MZ,Zeng MS,Xia YF

    更新日期:2012-04-01 00:00:00

  • Determinants of the interval between diagnosis and treatment in patients with digestive tract cancer.

    abstract::The main objective was to analyze the influence of social factors on the interval from diagnosis to treatment in a cohort of 217 patients with digestive tract cancer. From the clinical charts data were obtained for: date of the beginning of illness, dates of medical attendance, type and date of diagnostic tests, chara...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Mariscal M,Llorca J,Prieto-Salceda D,Palma S,Delgado-Rodríguez M

    更新日期:2003-03-01 00:00:00

  • Lung adenocarcinoma resistance to therapy with EGFR‑tyrosine kinase inhibitors is related to increased expression of cancer stem cell markers SOX2, OCT4 and NANOG.

    abstract::The present study aimed to explore the relationship between the efficacy of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR‑TKIs) and the expression of the cancer stem cell (CSC)‑related markers. Specimens of 72 cases of lung adenocarcinoma with significantly different therapeutic effects of the firs...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7454

    authors: Hu F,Li C,Zheng X,Zhang H,Shen Y,Zhou L,Yang X,Han B,Zhang X

    更新日期:2020-02-01 00:00:00

  • Complementation of temperature tolerance by rat Rgl-1 recessive oncogene in the absence of Saccharomyces cerevisiae Sop genes.

    abstract::It has been demonstrated that homozygous mutations at the L(2)gl locus in Drosophila result in the development of tumor in the presumptive adult optic centers of the larval brain and of the imaginal discs. We previously cloned an L(2)gl homologue, Rgl-1, in the rat brain. In this study, we analyzed the capability of R...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Kim YK,Kim YS,Chung HM,Baek KH

    更新日期:2004-11-01 00:00:00

  • Paris saponin II inhibits human ovarian cancer cell-induced angiogenesis by modulating NF-κB signaling.

    abstract::The clinical applications of Rhizoma paridis in traditional Chinese medicine are well known. However, the therapeutic potential of Rhizoma paridis and its active component such as Paris saponin I (polyphyllin D) and Paris saponin II (PSII) (formosanin C) in cancer treatments have not yet been fully explored. Recent st...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.3836

    authors: Yang M,Zou J,Zhu H,Liu S,Wang H,Bai P,Xiao X

    更新日期:2015-05-01 00:00:00

  • Core‑shell type thermo‑nanoparticles loaded with temozolomide combined with photothermal therapy in melanoma cells.

    abstract::A novel core‑shell type thermo‑nanoparticle (CSTNP) co‑loaded with temozolomide (TMZ) and the fluorescein new indocyanine green dye IR820 (termed IT‑CSTNPs) was designed and combined with a near‑infrared (NIR) laser to realize its photothermal conversion. The IT‑CSTNPs were prepared using a two‑step synthesis method a...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7329

    authors: Hou X,Pang Y,Li X,Yang C,Liu W,Jiang G,Liu Y

    更新日期:2019-12-01 00:00:00

  • Targeting ribonucleotide reductase M2 subunit by small interfering RNA exerts anti-oncogenic effects in gastric adenocarcinoma.

    abstract::Ribonucleotide reductase M2 subunit (RRM2) is one of the two subunits of human ribonucleotide reductase which plays a critical role in tumor progression. The aim of the present study was to analyze its expression, clinical significance and biological functions in gastric adenocarcinoma. We observed the upregulation of...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3148

    authors: Kang W,Tong JH,Chan AW,Zhao J,Wang S,Dong Y,Sin FM,Yeung S,Cheng AS,Yu J,To K

    更新日期:2014-06-01 00:00:00

  • USP14 activation promotes tumor progression in hepatocellular carcinoma.

    abstract::To elucidate the molecular mechanisms underlying the pathogenesis and treatment of human primary hepatocellular carcinoma (HCC), it is important to explore novel HCC-associated genes. In the present study, we examined the expression of ubiquitin-specific peptidase 14 (USP14) in patients with HCC using quantitative PCR...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4296

    authors: Huang G,Li L,Zhou W

    更新日期:2015-12-01 00:00:00

  • Purvalanol induces endoplasmic reticulum stress-mediated apoptosis and autophagy in a time-dependent manner in HCT116 colon cancer cells.

    abstract::Purvalanol, a novel cyclin-dependent kinase inhibitor, is referred to as a strong apoptotic inducer which causes cell cycle arrest in various cancer cells such as prostate, breast and colon cancer cell lines. Various physiological and pathological conditions such as glucose starvation, inhibition of protein glycosylat...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.3918

    authors: Coker-Gürkan A,Arisan ED,Obakan P,Akalın K,Özbey U,Palavan-Unsal N

    更新日期:2015-06-01 00:00:00

  • Human erythropoietin increases the pro-angiogenic potential of A2780 ovarian adenocarcinoma cells under hypoxic conditions.

    abstract::Erythropoietin (Epo) is a key regulator of erythroid cell proliferation, differentiation and apoptosis. In the form of the recombinant protein, it is widely used to treat various types of anemias, including that associated with cancer and with the myelosuppressive effects of chemotherapy, particularly platinum-based r...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2566

    authors: Kriška J,Solár P,Varinská L,Solárová Z,Kimáková P,Mojžiš J,Fedoročko P,Sytkowski AJ

    更新日期:2013-09-01 00:00:00

  • DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60).

    abstract::Vaccine strategies for the treatment of human papillomavirus-induced cervical cancer are based mainly on the human papillomavirus 16 E7 (HPV16 E7) oncoprotein. The immunogenicity of the E7 gene has been enhanced by its fusion to many different genes. Here, we linked a short sequence coding for the E7 peptide (aa 44-60...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Pokorna D,Cerovska N,Smahel M,Moravec T,Ludvikova V,Mackova J,Synkova H,Duskova M,Hozak P,Veleminsky J

    更新日期:2005-10-01 00:00:00

  • Establishment and characterization of a human adenoid cystic carcinoma cell line forming colonies cultured in collagen gel and transplantable in nude mice.

    abstract::We established a new cell line (ACCNS) from human adenoid cystic carcinoma (AdCC) of the maxilla, using tissue culture techniques and it has been successfully subcultured for more than 100 passages during 2 years. The population doubling time was approximately 39.2 h on a plastic dish. In type I collagen gel culture, ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Tanaka N,Urabe K,Hashitani S,Sakurai K,Urade M

    更新日期:2007-02-01 00:00:00

  • Analysis of T-helper type 1 and 2 cells and T-cytotoxic type 1 and 2 cells of sentinel lymph nodes in breast cancer.

    abstract::The immune suppression of sentinel lymph node (SN) is directly influenced by primary tumors. It has been reported that the T-helper type 1 (Th1) to T-helper type 2 (Th2) ratio or T-cytotoxic type 1 (Tc1) to T-cytotoxic type 2 (Tc2) ratio of lymph node lymphocytes could be used to evaluate direct immunological circumst...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Ehi K,Ishigami S,Masamoto I,Uenosono Y,Natsugoe S,Arigami T,Arima H,Kijima Y,Yoshinaka H,Yanagita S,Kozono T,Funasako Y,Maruyama I,Aikou T

    更新日期:2008-03-01 00:00:00

  • Antitumor activity of monomethoxy poly(ethylene glycol)-poly (ε-caprolactone) micelle-encapsulated doxorubicin against mouse melanoma.

    abstract::Doxorubicin (Dox) is one of the most commonly used and highly effective antineoplastic agents, but the clinical application of this broad spectrum drug is largely hampered by its poor stability and serious toxicity to normal tissues. Hence, it is essential to improve the therapeutic effect and decrease the systematic ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1243

    authors: Zheng L,Gou M,Zhou S,Yi T,Zhong Q,Li Z,He X,Chen X,Zhou L,Wei Y,Qian Z,Zhao X

    更新日期:2011-06-01 00:00:00