Abstract:
BACKGROUND:Studying a process in a new species often relies on focusing our attention to a candidate gene, encoding a protein similar to one with a known function. Not all the choices seem to be prudent. SCOPE:This Viewpoint includes an overview of issues that are encountered during research of candidate genes. Defining a match for a gene of interest, deciding whether variation in ESTs or RNAseq data for a certain transcript, represent more than one gene. The problem of incorrect annotation of genes due to incorrect in-silico splicing, is also mentioned. The author's humble opinion on how to deal with these issues is provided. CONCLUSIONS:The vast amount of new sequence data provides us with great possibilities for giant leaps in our understanding. Still, we cannot afford to skip over the tedious steps required to confirm that we are indeed studying the correct gene, and try to be sure that the complex expression pattern we observe is not a composite of several genes.
journal_name
Ann Botjournal_title
Annals of botanyauthors
Samach Adoi
10.1093/aob/mcs161subject
Has Abstractpub_date
2013-03-01 00:00:00pages
329-33issue
3eissn
0305-7364issn
1095-8290pii
mcs161journal_volume
111pub_type
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