Abstract:
OBJECTIVE:In this study, we investigated the developmental potential of single blastomeres that were obtained from 4-cell mice embryos that were split during the blastocyst stage. MATERIALS AND METHODS:Imprinting Control Region (ICR) mice (age: 6-8 weeks), were superovulated and mated with a single fertile male of the same strain. We obtained 2-cell embryos that were then cultured in 4 groups (×4) with Human tubal fluid (HTF) supplemented with 12% fetal bovine serum. When these embryos reached the 4-cell stage, their zonae pellucidae were removed and every single blastomere was isolated by repeated pipetting with Ca/Mg(2+)-free medium. The isolated blastomeres (study group) and the intact embryos (control group) were then cultured to determine the blastocyst formation rate and quality. RESULTS:We collected a total of 936 embryos from 524 morphologically intact, top-grade embryos in the 4-cell stage from 80 stimulated mice. We used 356 of these embryos to isolate the blastomeres. The remaining 168 embryos were cultured as controls. A total of 1312 single blastomeres were obtained and cultured in vitro. Among these, 620 blastocysts were harvested from the original embryos compared with 136 blastocysts that were harvested from the control group. The overall blastocyst formation rate was 174.2% (620 blastocysts from 356 embryos) for the study group compared with 81.5% (136 blastocysts from 168 embryos) for the control group. The study group was 43.3% (268 of 620) top-grade blastocysts compared with 91% (152 of 168) of the control group. Taken together, the percentage of top-grade blastocysts obtained per original embryo in the split group was 75.4% (174.2%×43.3%) compared with 74.2% (81.5%×91%) for the control group. CONCLUSIONS:Embryo splitting can increase the number of blastocysts. However, the percentage of available top-grade blastocysts is the same compared with nonsplit embryos. Embryo splitting may not be a cost-effective technique for the generation of high-quality mouse blastocysts.
journal_name
Taiwan J Obstet Gynecoljournal_title
Taiwanese journal of obstetrics & gynecologyauthors
Tang HH,Tsai YC,Kuo CTdoi
10.1016/j.tjog.2012.04.012subject
Has Abstractpub_date
2012-06-01 00:00:00pages
236-9issue
2eissn
1028-4559issn
1875-6263pii
S1028-4559(12)00068-Xjournal_volume
51pub_type
杂志文章abstract:OBJECTIVE:We aimed to assess the effects of obesity-related follicular fluid (FF) fatty acids (FAs) on the number and quality of oocytes, good embryo quality rate, and pregnancy rate. MATERIALS AND METHODS:This prospective cohort study was conducted on 105 infertile women under the age of 38, who underwent intracytopl...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2016.11.005
更新日期:2017-04-01 00:00:00
abstract:OBJECTIVE:This study aims to evaluate the role of kisspeptin-10 in preeclampsia and check for possible relationship between its severity and fetal growth well-being. Kisspeptin-10 may participate in implantation of the embryo, placenta formation, and maintenance of pregnancy. MATERIALS AND METHODS:One hundred women wh...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2015.10.028
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVE:Loeys-Dietz syndrome (LDS) is associated with a higher risk of aortic dissections (ADs) during pregnancy and postpartum. However, there is limited evidence about the perinatal management of LDS patients who have undergone prophylactic aortic root replacements (ARRs) before conception. CASE REPORT:We present ...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type:
doi:10.1016/j.tjog.2020.10.005
更新日期:2021-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the prevalence of daytime urinary frequency among Taiwanese women aged 60 years and older. MATERIALS AND METHODS:There were an estimated 1.25 million female residents aged 60 years and over in Taiwan in 2000. A sample of 2,410 women was selected using a multistage random sampling method. Face-to-...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/S1028-4559(09)60328-4
更新日期:2009-12-01 00:00:00
abstract:OBJECTIVE:To present prenatal diagnosis of mosaic trisomy 2. MATERIALS AND METHODS:A 29-year-old woman underwent amniocentesis at 17 weeks of gestation because of abnormal maternal serum screening, and the cytogenetic result was 47,XY,+2[8]/46,XY[22]. She underwent repeated amniocentesis at 19 weeks of gestation. Inte...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2013.06.004
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVE:This study aimed to compare the outcomes of magnetic resonance-guided (MR-g) high-intensity focused ultrasound (HIFU) with uterine artery embolization (UAE) for the treatment of uterine myoma. MATERIALS AND METHODS:A systematic literature review and meta-analysis was conducted using database searches of Medl...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2020.07.012
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVE:This study was conducted to compare the efficacy of cryotop vitrification of human cleavage-stage embryos to that of conventional slow freezing of these embryos with respect to survival. A second objective was to compare the two cryopreservation techniques with respect to outcomes for a cohort of women. MATE...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/S1028-4559(10)60060-5
更新日期:2010-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the efficacy and toxicity of VMP regimen applied to the patients with low-risk gestational trophoblastic neoplasia (LR-GTN) treated in Anhui provincial hospital. MATERIALS AND METHODS:Between 2005 and 2017, 87 patients with low-risk gestational trophoblastic neoplasia received VMP regimen, consis...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2019.03.008
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVE:It is known that most cases of ovarian hyperstimulation syndrome (OHSS) are associated with the therapies for ovulation induction. However, OHSS may rarely be associated with a spontaneous ovulatory cycle, usually in the case of multiple gestations, hypothyroidism or polycystic ovary syndrome. CASE REPORT:A ...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/S1028-4559(09)60241-2
更新日期:2006-09-01 00:00:00
abstract:OBJECTIVE:This prospective study presents a preliminary result to compare the clinical efficacy of patients with stress urinary incontinence and mixed urinary incontinence using minimal invasive Er:YAG vaginal laser. MATERIALS AND METHODS:A total of 20 patients were included, in which were 10 patients with SUI and 10 ...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2019.07.006
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVE:To analyze the benefits of external cephalic version (ECV) with epidural analgesia at term and labor induction just after the procedure. MATERIALS AND METHODS:This is a retrospective observational study with patients who did not want trying a breech vaginal delivery and decided trying an ECV with epidural an...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2017.04.018
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVE:To discuss the follow-up and management of fetal ovarian cysts (FOCs) and review the current literature. CASE REPORT:A 26-year-old, gravida 2, para 0, abortus 1, Rh-negative patient was diagnosed with bilateral FOCs during ultrasound examination at 32 weeks gestation. Fetal nuchal translucency measured at 12...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/s1028-4559(08)60084-4
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:The purpose of the study was to compare the Quinn's Advantage fertilization medium (Q1) and the tissue culture medium 199 (TCM199) for in vitro maturation (IVM) of oocytes and ammonium production during IVM. MATERIALS AND METHODS:The immature murine oocytes were randomly added into Q1 and TCM199. Ammonium co...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2013.12.001
更新日期:2014-03-01 00:00:00
abstract:OBJECTIVE:To assess the clinical significance of low compliance bladder (LCB) in women with lower urinary tract symptoms. MATERIALS AND METHODS:Medical records of 1490 women undergoing videourodynamic studies (VUSs) were reviewed. Comprehensive medical histories, physical examinations, bladder diaries, and results of ...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2015.10.003
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVE:To estimate the prevalence of nocturia in Taiwanese women aged 20-59 years. MATERIALS AND METHODS:A random sample of 4,549 women within the age range was selected by a multistage sampling design. Face-to-face visits with 3,537 women were completed by well-trained professional interviewers. Nocturia, accordin...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/S1028-4559(08)60107-2
更新日期:2007-03-01 00:00:00
abstract:OBJECTIVE:We describe a case of gestational choriocarcinoma metastasized to the kidney and lung, which presented initially as refractory hematuria after a term pregnancy 5 years earlier. CASE REPORT:A 35-year-old woman, G2P1, with a previous history of full-term pregnancy in 2009, presented to the emergency department...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2015.08.028
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:To investigate the risks of increasing maternal age on the perinatal and obstetric outcomes. MATERIALS AND METHODS:Information about 29,760 singleton pregnancies delivered between 2005 and 2008 was extracted from our database. Patients were categorized into four groups according to age: 20-29 years, 30-34 y...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2012.01.012
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to describe our experience with the diagnosis and management of acquired uterine vascular abnormalities associated with persistent human chorionic gonadotropin (hCG). Through this case series, we sought to establish our protocol for the treatment and follow-up of uterine vascular les...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2014.06.008
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVE:We present prenatal diagnosis of low-level mosaicism for tetrasomy 18p at amniocentesis in a pregnancy with a favorable outcome. CASE REPORT:A 40-year-old woman underwent amniocentesis at 17 weeks of gestation because of advanced maternal age. Amniocentesis revealed a de novo supernumerary isochromosome 18p ...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2017.10.024
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVES:To study the operative outcomes of single-port laparoscopic hysterectomy in uteri greater than 500 g, using either laparoscopic assisted vaginal hysterectomy (LAVH) or laparoscopic supracervical hysterectomy (LSH). MATERIALS AND METHODS:78 patients were included in a single institute by a single surgeon fro...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2020.05.006
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVE:Existing drug-induced abortion techniques involve oral administration of 200 mg of mifepristone, followed by oral administration of 600 microg of misoprostol 48 hours later, but the effects are variable. As revealed by recent research, sublingual and oral administrations of misoprostol are equally efficacious...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 临床试验,杂志文章
doi:10.1016/S1028-4559(09)60251-5
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVE:Obstetrician-gynecologists are the main providers of women's healthcare. However, workforce shortages and excessive workloads among these providers have been encountered in many countries. While most past studies on this subject have investigated the spatial distribution of obstetrics-gynecology clinics, few ...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2017.08.011
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVE:To understand the mechanisms of postpartum uterine involution, we investigated the uterine myometrial changes during pregnancy and the postpartum period. MATERIALS AND METHODS:Nine groups of uterine myometrial samples from mice (n = 4) were collected on gestational Day 0 (nonpregnant), Day 1, Day 2, Day 7, D...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2013.01.030
更新日期:2014-09-01 00:00:00
abstract::Laparoscopic hysterectomies increase recently due to several advantages of minimally invasive surgery. Controversy exists for laparoscopic hysterectomies for large uteri weighing >500g because some reports show increased complications and morbidities and high laparoconversion rate in the past. With familiarity of lapa...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章,评审
doi:10.1016/j.tjog.2011.10.003
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVE:To present molecular cytogenetic characterization of a de novo duplication of Xq22.1→q24 in a mentally retarded woman with short stature and premature ovarian failure. MATERIALS AND METHODS:A 19-year-old woman presented with psychomotor retardation, developmental delay, mental retardation, short stature, low...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2011.01.018
更新日期:2011-09-01 00:00:00
abstract::Breast-feeding initiation within the first half hour after birth is one of the World Health Organization recommendations. However, in most hospitals, mother-infant contact and breast-feeding initiation are delayed due to routine mother and infant care. This study aimed to determine the effect of mother-infant skin to ...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章,meta分析
doi:10.1016/j.tjog.2018.11.002
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:We present the application of non-invasive prenatal testing (NIPT) in late gestation in a pregnancy associated with intrauterine growth restriction (IUGR) and trisomy 22 confined placental mosaicism (CPM). CASE REPORT:A 35-year-old pregnant woman underwent chorionic villus sampling (CVS) at 12 weeks of gesta...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2017.09.001
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVE:We present detection of paternal origin of fetal trisomy 21 in a pregnancy with isolated ventriculomegaly but without advanced parental age. CASE REPORT:A 29-year-old pregnant woman was admitted to the hospital at 18 weeks of gestation for tocolytic treatment because of irregular uterine contractions. Her hu...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type:
doi:10.1016/j.tjog.2020.05.025
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVE:Difficulties often encountered during intubation in extremely low birth-weight (ELBW) neonates requiring resuscitation at birth because of the smaller airway and the pressure from the limited number of attempts before hemodynamic instability occurs. CASE REPORT:We evaluated two pregnant women at 26 weeks of ...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/j.tjog.2017.07.001
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVE:To present prenatal diagnosis and molecular genetic analyses of mosaic trisomy 7. MATERIALS, METHODS AND RESULTS:A 38-year-old primigravid woman underwent amniocentesis at 19 weeks of gestation because of her advanced maternal age. Amniocentesis revealed a karyotype of 47,XY,+7[26]/46, XY[16]. Repeated amnio...
journal_title:Taiwanese journal of obstetrics & gynecology
pub_type: 杂志文章
doi:10.1016/S1028-4559(10)60070-8
更新日期:2010-09-01 00:00:00