Abstract:
OBJECTIVE:To investigate the efficiency of β-galactosidase gene transfer into rat kidney with ultrasound-mediated microbubble destruction via different injection routes. METHODS:A total of 25 Wistar rats were randomly divided into 5 groups. Four groups received a mixture of optison microbubbles (0.2 mL) and lacz plasmids (25 μg) injection via renal artery, tail vein, anterior tibial muscle and renal parenchyma, respectively. The control group received a mixture of PBS (xx mL) and lacz plasmids (25 μg) via renal artery. Three days after the gene transfer, ultrasound with fixed frequency and power (1 MHz, xxW) was delivered to the kidneys for 3 min. The efficiency of the gene transfer and expression was evaluated on the basis of β-galactosidase expression. The side effects of this method were evaluated by immunohistological method. RESULTS:β-galactosidase expression could be observed only in tubules but not in glomeruli and interstitial area. The efficiency of renal artery group was higher than that of tail vein, anterior tibial muscle and renal parenchyma group (P<0.05). Immunohistochemical analysis revealed co-expression of β-galactosidase with a roximal tubule marker, megalin, which suggested that ultrasound enhanced gene transfer into the proximal tubular epithelial cells. No β-galactosidase expression was observed in the extrarenal organs. There were no evident pathological and biochemical changes after gene transfer. CONCLUSIONS:Ultrasound-mediated microbubble destruction can transfer gene into kidney via renal artery, tail vein, anterior tibial muscle and renal parenchyma. Compared with renal artery, administrating microbubbles via tail vein and anterior tibial muscle are more convenient and less vulnerarious.
journal_name
Asian Pac J Trop Medjournal_title
Asian Pacific journal of tropical medicineauthors
Chen JX,Ma Q,Wu H,Zhou A,Chen X,Peng YM,Liu FY,Cheng MCdoi
10.1016/S1995-7645(12)60099-6subject
Has Abstractpub_date
2012-07-01 00:00:00pages
561-5issue
7eissn
1995-7645issn
2352-4146pii
S1995-7645(12)60099-6journal_volume
5pub_type
杂志文章abstract::Tuberculosis (TB) is a symbolic menace to mankind, infecting almost one third of the world's populace and causing over a million mortalities annually. Mycobacterium tuberculosis (Mtb) is the key pathogen of TB that invades and replicates inside the host's macrophage. With the emerging dilemma of multi-drug resistant t...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章,评审
doi:10.1016/j.apjtm.2016.09.005
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVE:To elucidates the immunoprophylactic potential of glutathion-s-transferase (GST) from cattle filarial parasite Setaria digitata (S. digitata) against lymphatic filariasis. METHODS:GST was purified through affinity chromatography (SdGST) and chacterized by SDS-PAGE and Nano-LC MS/MS analysis. Antibody isotype...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60066-7
更新日期:2011-03-01 00:00:00
abstract::A natural prodrug is a chemical compound or substance obtained from plants, microorganism, animal and marine sources. Natural products are small molecule source for Food and Drug Administration approved drugs and major sources for drug discovery. Most of the drugs for different ailment diseases undergo first pass meta...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60203-0
更新日期:2014-09-01 00:00:00
abstract::Tropical pyomyositis is an underdiagnosed condition. We reported a 35 year old male farmer, who presented with septicemia and acute respiratory distress syndrome due to pyomyositis involving the paraspinal muscles. Culture of the pus grew methicillin sensitive Staphylococcus aureus, and the patient recovered after sur...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60096-5
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVE:To detect neurovascular compression-induced structural abnormalities of trigeminal nerves (TGN) by diffusion tensor imaging (DTI). METHODS:The affected ipsilateral TGN (iTGN) and unaffected contralateral TGN (cTGN) of 20 trifacial neuralgia (TN) patients as well as the bilateral TGN of 10 normal controls (nT...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60119-9
更新日期:2012-09-01 00:00:00
abstract:OBJECTIVE:To evaluate efficiency of HAART in the prevention of mother to child HIV transmission. METHODS:A longitudinal study was conducted on 1 300 women attending the antenatal service at Saint Camille Medical Centre from September 2010 to July 2011. The HIV status of mothers was determined by rapid tests and ELISA....
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60188-6
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVE:To invest the differences among mesenchymal stem cells (MSCs) derived from different tissues and their impacts on clinical applications. METHODS:In this study, MSCs were isolated from adipose tissue (AD), umbilical cord tissue (UC), and menstrual blood (Men) and compared their biological characteristics in t...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2015.07.022
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of pomegranate peel aqueous extract (PGE) on the development of secondary experimental echinococcosis and on the viability of Echinococcus granulosus protoscoleces, and the immunomodulatory properties of PGE. METHODS:Swiss mice were inoculated intraperitoneally with viable protoscol...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2016.01.038
更新日期:2016-03-01 00:00:00
abstract:OBJECTIVE:To investigate the preventive and therapeutic effect and mechanism of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage. METHODS:Sixty SD rat aged 9-12 weeks were chosen and divided into the control group, model group and simvastatin-treated group randomly with 20 rats in each gro...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2017.01.003
更新日期:2017-02-01 00:00:00
abstract::Salmonella are most commonly associated with gastroenteritis and enteric fever in humans. Occasionally, dissemination of bacilli throughout the body results in establishment of localized foci of persistent infection especially in patients with debilitating diseases and immunosuppressive states. Infection at various ab...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60117-X
更新日期:2011-05-01 00:00:00
abstract:OBJECTIVE:To determine the relationship between asymptomatic malaria parasitemia and some oxidative stress parameters in pregnant Nigerian women. METHODS:This is a cross-sectional study involving 130 normal pregnant women at various trimesters, who were attending antenatal clinic at the University of Nigeria Teaching ...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60076-X
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:To determine Blastocystis frequency and subtypes (ST) in ulcerative colitis (UC) patients and analyse some laboratory findings between Blastocystis positive and negative cases. METHODS:Faecal samples from 150 UC patients in Adnan Menderes University, Training and Research Hospital were examined by direct mic...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2016.07.018
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVE:To investigate the effects of ethanolic extract of Lagenaria siceraria fruit (ELSF) on fat amassment and serum TNF- α in high-fat diet-induced obese rats. METHODS:The high fat diet induced obese rats were orally treated with orlistat (50 mg/kg) and ELSF (100, 200, 300 mg/kg/day) to the respective treatment g...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60109-6
更新日期:2012-09-01 00:00:00
abstract:OBJECTIVE:To explore the regulation effect of Rheum palmatum (R. palmatum) L. on NF-κB signaling pathway of ALF mice. METHODS:The intraperitoneal injection of d-GalN/LPS was employed for the model building. Mice in the treatment group and positive control group were given the R. palmatum L. and bifendate before the mo...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2015.09.011
更新日期:2015-10-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of Cannabis sativa resin and/or tramadol, two commonly drugs of abuse on acetylcholinesterase and butyrylcholinesterase activities as a possible cholinergic biomarkers of neurotoxicity induced by these agents. METHODS:Rats were treated with cannabis resin (5, 10 or 20 mg/kg) (equiva...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2016.09.009
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVE:To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus (E. granulosus) and measuring polyclonal antibody and humoral immune response using ELISA and gel diffusion. METHODS:Two mentioned antigens were cloned and expressed in expression vector and purified by affi...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(12)60058-3
更新日期:2012-05-01 00:00:00
abstract:OBJECTIVE:To investigate the propensity of plumbagin to inhibit the three isoforms of human cytochrome P450 (CYP), i.e., CYP1A2, CYP2C19, and CYP3A4 using human liver microsomes in vitro. METHODS:Inhibitory effects of plumbagin on the three human CYP isoforms were investigated using pooled human liver microsomes. Phen...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2015.10.016
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:To investigate the role of human host heme-oxygenase-1 (HO-1) in pathogenesis of cerebral malaria in the in vitro model. METHODS:The effect of human host HO-1 [human brain microvascular endothelial cell (HBMEC)] on hemoglobin degradation in the co-culture model of HBMEC and ITG Plasmodium falciparum-infected...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2016.11.011
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:To describe the epidemiology of snake bite in the region and attempt to compare proven Russell's viper with hump-nosed viper bites. METHODS:All snake bite admissions to the Toxicology Unit of Teaching Hospital Peradeniya over three year from 2006 were included. RESULTS:Of the 776 snakebites, 665(86%) were u...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60147-8
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVE:To report presence of Leishmania major in Khyber Pakhtunkhwa of Pakistan, where cutaneous leishmaniasis (CL) is endemic and was thought to be caused by Leishmania tropica only. METHODS:Biopsy samples from 432 CL suspected patients were collected from 3 southern districts of Khyber Pakhtunkhwa during years 20...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2017.07.015
更新日期:2017-07-01 00:00:00
abstract:OBJECTIVE:To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva lanata (A. lanata) stem. METHODS:During the preliminary phytochemical analysis, the aqueous extract of A. lanata was screened for the presence of carbohydrates, proteins, phenolic compounds, oil and fats,...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(13)60020-6
更新日期:2013-03-01 00:00:00
abstract:OBJECTIVE:To confirm dengue infection among Russian tourists returned from Southeast and Mexico in 2010-2013 with clinical signs of infection. METHODS:Blood and serum samples from patients were collected. NS1 antigen and human IgM/IgG antibodies to dengue virus were identified using commercial tests manufactured by "S...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60194-2
更新日期:2015-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the curative effect of the 132 KD protein isolated from the seeds of Peganum harmala (P. harmala) L. against carbon tetrachloride (CCl(4)) induced oxidative stress in rats. METHODS:Animals were post treated intraperitoneally with 132 KD isolated protein at doses of 4 and 8 mg/kg body weight and b...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(13)60058-9
更新日期:2013-04-13 00:00:00
abstract:OBJECTIVE:To investigate the effect of the anthocyanin-rich extract of Hibiscus sabdariffa (H. sabdariffa) calyx on the viability of cadmium-treated U937 cells and cadmium-mediated activation of U937-derived macrophages. METHODS:The macrophage cell line U937 was treated with cadmium (0.1 μ mol/L) and later incubated w...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(11)60241-1
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:To optimize the process parameters involved in the green synthesis of silver nanoparticles (G-SNPs) by aqueous extract of Rosa damascena petals and to evaluate the biocompatibility and anti cancer activity of the synthesized silver nanoparticles against human lung adenocarcinoma (A549). METHODS:The process v...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60249-2
更新日期:2014-09-01 00:00:00
abstract::We present an atypical case of disseminated cutaneous leishmaniasis in the Sihuas district, located in the Andean valleys of Ancash-Peru. A 62-year-old man with no particular medical history presented multiple lesions located on the inferior abdomen, lumbar region and the right anterior thigh. Histological analysis fo...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2017.10.001
更新日期:2017-11-01 00:00:00
abstract:OBJECTIVE:To establish the prevalence and associated risk factors of Schistosoma mansoni (S. mansoni) infection among schoolchildren at a village in Wolaita Zone, Southern Ethiopia. METHODS:A cross-sectional study was carried out among primary schoolchildren. A total of 384 randomly selected study subjects provided st...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2015.05.009
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVE:To induce pluripotent stem (IPS) cells from fibrocytes that are separated from liver cancer patients. METHODS:The fibrocytes were reprogrammed to IPS cells by lentiviral vector, stained and identified by immunohistochemistry. RESULTS:The IPS cells were successfully established from fibrocytes after infectio...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/S1995-7645(14)60032-8
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVE:To investigate the mechanism of antibacterial activity of luteolin (LUT) against methicillin-resistant Staphylococcus aureus (MRSA). METHODS:The mechanism of anti-MRSA activity of LUT was analyzed by the viability assay in membrane permeabilizing agent, ATPase inhibitors, and peptidoglycan (PGN) derived from...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2015.12.004
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVES:To evaluate possible lipid catabolism and body fat regulation effects of 3-caffeoylquinic acid in Green coffee bean extract (GCBE) in high-fat diet (HFD)-induced obese mice. METHODS:Obesity was induced in mice using a HFD for four weeks. Then, mice were fed only HFD or HFD with GCBE at 50, 100, and 200 mg/k...
journal_title:Asian Pacific journal of tropical medicine
pub_type: 杂志文章
doi:10.1016/j.apjtm.2016.05.017
更新日期:2016-07-01 00:00:00